Reactive oxygen species: response of algal cells

Mallick, Nirupama; Mohn, Friedrich Helmuth

doi:10.1016/s0176-1617(00)80189-3

Cited by 390 publications

(249 citation statements)

References 68 publications

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“…11,55 This decrease in reduced GSH has significant impacts on cells, as most cellular processes utilise the reduced form. 56 The use of XANES in this study also highlighted differences in the Cu-binding ligand in Cu-exposed cells for the two diatoms C. closterium and P. tricornutum supporting the hypothesis that the two diatoms have different intracellular mechanisms for sequestering excess Cu.…”

Section: Discussionsupporting

confidence: 70%

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Adams

Dillon

Vogt

et al. 2016

Environ. Sci. Technol.

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Metal toxicity to aquatic organisms depends on the speciation of the metal and its binding to the critical receptor site(s) (biotic ligand) of the organism. The intracellular nature of the biotic ligand for Cu in microalgal cells was investigated using the high elemental sensitivity of microprobe synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption near-edge spectroscopy (XANES). The marine microalgae, Ceratoneis closterium, Phaeodactylum tricornutum, and Tetraselmis sp. were selected based on their varying sensitivities to Cu (72-h 50% population growth inhibitions of 8–47 μg Cu/L). Intracellular Cu in control cells was similar for all three species (2.5–3.2 × 10–15 g Cu/cell) and increased 4-fold in C. closterium and Tetraselmis sp. when exposed to copper, but was unchanged in P. tricornutum (72-h exposure to 19, 40, and 40 μg Cu/L, respectively). Whole cell microprobe SR-XRF identified endogenous Cu in the central compartment (cytoplasm) of control (unexposed) cells. After Cu exposure, Cu was colocated with organelles/granules dense in P, S, Ca, and Si and this was clearly evident in thin sections of Tetraselmis sp. XANES indicated coexistence of Cu(I) and Cu(II) in control and Cu-exposed cells, with the Cu ligand (e.g., phytochelatin) in P. tricornutum different from that in C. closterium and Tetraselmis sp. This study supports the hypothesis that Cu(II) is reduced to Cu(I) and that polyphosphate bodies and phytochelatins play a significant role in the internalization and detoxification of Cu in marine microalgae.

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Section: Discussionsupporting

confidence: 70%

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Adams

Dillon

Vogt

et al. 2016

Environ. Sci. Technol.

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“…In order to mitigate the oxidative stress caused by ROS, organisms have developed an effective antioxidant system including antioxidant enzymes such as SOD, CAT and POD [30,28], which are important antioxidant defenses against photogenerated free radicals. Their activity and role are probably crucial for the survival of M. vaginatus and protection against bleaching since the UV-B intensity during daytime hours under natural conditions is much higher than that in our experiments.…”

Section: Discussionmentioning

confidence: 99%

Ultraviolet-B exposure induces photo-oxidative damage and subsequent repair strategies in a desert cyanobacterium Microcoleus vaginatus Gom.

Xie

Wang

Liu

et al. 2009

European Journal of Soil Biology

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“…Under stress conditions these redox signals could interfere with signalling networks complementary to the antioxidant system and regulate defence gene expression, thus coordinating the necessary readjustments in the redox-regulated plant defence to overcome the oxidative stress (Mittler et al 2004;Kuzniak and Sklodowska 2005). Additionally, CAT eliminates H 2 O 2 by breaking it down to H 2 O and O 2 and does not require any reducing equivalent (Mallick and Mohn 2000). GPX is considered to be an important ROS scavenger because of its broader substrate specifications and stronger affinity for H 2 O 2 than those of catalase (Brigelius-Flohe and Flohe 2003).…”

Section: Introductionmentioning

confidence: 99%

Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

Hossain

Hasanuzzaman

Fujita

2010

Physiol Mol Biol Plants

373

162

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Reactive oxygen species: response of algal cells

Cited by 390 publications

References 68 publications

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Ultraviolet-B exposure induces photo-oxidative damage and subsequent repair strategies in a desert cyanobacterium Microcoleus vaginatus Gom.

Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

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