Reactive oxygen species trigger the fast action of glufosinate

Takano, Hudson Kagueyama; Beffa, Roland; Preston, Christopher; Westra, Philip; Dayan, Franck E.

doi:10.1007/s00425-019-03124-3

Cited by 85 publications

(122 citation statements)

References 35 publications

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“…More recently, a third reason for the toxicity of PPT to plants has been proposed. Takano et al [16] demonstrated that reactive oxygen species are the main driver for rapid cell death after glufosinate treatment resulted in ammonium accumulation, and changes in amino acid levels are probably a secondary effect of the GS inhibition. A similar assumption was previously made by Merino et al [33], which linked increased resistance to PPT in hybrid poplar expressing small heat shock protein (sHSP) gene with general protection of proteins and membranes against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…However, the results of a number of subsequent studies called into question this conclusion since the addition of amino acids weakened the negative effect of PPT [14,15]. To date, the reason for extremely rapid glufosinate-induced death of leaves is still unclear [16]. It should be noted that despite (or due to) the widespread use of both glyphosate and glufosinate in the world, currently, more than 40 species of glyphosate-tolerant weeds are known in the world [17], while glufosinate-tolerance are limited only to two species [18].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Phosphinothricin on Transgenic Downy Birch (Betula pubescens Ehrh.) Containing bar or GS1 Genes

Lebedev

Krutovsky

Shestibratov

2019

Forests

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Weeds are a big problem in agriculture and forestry, and herbicides are the main tools to control them. Phosphinotricin (ammonium glufosinate, PPT) is one of the most effective non-selective herbicides, to which weeds hardly gain resistance, but the reasons for its effect and toxicity to plants are still unclear, and especially, it is little studied in trees, including transgenic ones. We studied the physiological responses of downy birch (Betula pubescens Ehrh.) containing the herbicide resistance bar gene or the cytosol glutamine synthetase GS1 gene (the target enzyme of the herbicide) to PPT-based Basta herbicide treatment in various doses under open-air conditions during two years. Birch saplings with the bar gene were resistant to a double field dose (10 L/ha), but the expression of the GS1 gene only slightly increased resistance compared to the control. Herbicide treatment increased the ammonium level in leaf tissue by 3–8 times, but this, apparently, was not the main cause of plant death. Among leaf pigments, chlorophyll B was the most resistant to PPT, and carotenoids were the most sensitive. Responses of birch trees with the GS1 gene (accumulation of ammonium, pigment content, and dehydration) during treatment with a low dose of herbicide were less pronounced than in control plants. One-year-old control and transgenic plants with the GS gene died after 2.5 L/ha treatment, and two-year-old plants lost foliage after such treatment but remained alive and developed buds four weeks after treatment. Herbicide treatment of plants with the bar gene did not cause significant deviations in height (first year) or the accumulation of aboveground biomass (second year). The obtained results improve our understanding of the effect of PPT on woody plants and can be used both to clarify mechanisms of herbicide action and in plantation forestry.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Phosphinothricin on Transgenic Downy Birch (Betula pubescens Ehrh.) Containing bar or GS1 Genes

Lebedev

Krutovsky

Shestibratov

2019

Forests

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“…We posit that ROS remains a candidate for this signal because treatment with a low concentration of chlorosulfuron herbicide led to ROS accumulation in the leaf (Fig. 4E), as does exposure to glyphosate and glufosinate (Faus et al, 2015;Takano et al, 2019). Moreover, the antioxidant ascorbate could delay the activation of GCN2 kinase by excess light (Supplemental Figure 4).…”

Section: Herbicides Rely On Photosynthetic H2o2 To Activate Gcn2mentioning

confidence: 92%

Light activates the translational regulatory GCN2 kinase via reactive oxygen species emanating from the chloroplast

Lokdarshi

Guan

Camacho

et al. 2019

Preprint

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Word count (main text excluding legends, references): 7,751, 12 figures. ABSTRACTCytosolic mRNA translation is subject to global and mRNA-specific controls. Phosphorylation of translation initiation factor eIF2a anchors a reversible switch that represses translation globally.The stress-responsive GCN2 kinase is the only known kinase for eIF2a in Arabidopsis. Here we show that conditions that generate reactive oxygen species (ROS) in the chloroplast, such as darklight transitions, high light, and the herbicide methyl viologen all rapidly activated the GCN2 kinase, whereas mitochondrial and ER stress did not. In addition, GCN2 activation was light dependent and mitigated by photosynthesis inhibitors and ROS quenchers. Accordingly, seedling growth of multiple gcn2 mutant alleles was retarded under conditions of excess light, implicating the GCN2-eIF2a pathway in responses to light and associated ROS. Once activated, the GCN2 kinase preferentially suppressed the ribosome loading of mRNAs for functions such as mitochondrial ATP synthesis, the chloroplast thylakoids, vesicle trafficking, and translation. The transcriptome of gcn2 mutants was sensitized to abiotic stress, including oxidative stress, as well as innate immune responses. Accordingly, gcn2 displayed defects in immune priming by the fungal elicitor, chitin. In conclusion, we provide evidence that reactive oxygen species produced by the photosynthetic apparatus help to activate the highly conserved GCN2 kinase, leading to eIF2a phosphorylation and thus affecting the status of the cytosolic protein synthesis apparatus.

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“…Estes sintomas são o resultado do estresse oxidativo iniciado por espécies reativas de oxigênio (EROs), como os radicais superóxido (O 2 -), radicais hidroxila (OH-), peróxido de hidrogênio (H 2 O 2 ) e oxigênio singleto ( 1 O 2 ) (GILL & TUTEJA 2010). As EROs reagem com lipídios formadores de membranas, causando a peroxidação lipídica (TAKANO et al 2019).…”

Section: Introductionunclassified

Interação do tratamento de sementes e da mistura em tanque de herbicidas na seletividade para o arroz irrigado

Catoni¹,

Neto

Riscarolli³

et al. 2020

Rev. Ciênc. Agrovet.

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Interação do tratamento de sementes e da mistura em tanque de herbicidas na seletividade para o arroz irrigadoInteraction of seed treatment and herbicide tank mixture in paddy rice selectivity RESUMO A elevada fitointoxicação após a aplicação de herbicidas tem se tornado cada vez mais frequentes em função da mistura em tanque de herbicidas praticada a campo, pressionadas pelo aumento do espectro de controle e pelo avanço da resistência de plantas daninhas a herbicidas. Objetivou-se, neste trabalho avaliar a interação do tratamento de sementes com uma formulação comercial de fungicida e inseticida com a aplicação de mistura em tanque de herbicidas no arroz irrigado, cultivado no sistema prégerminado, na região do Alto Vale do Itajaí, SC. O experimento foi conduzido em uma área comercial sistematizada para o sistema pré-germinado, com a cultivar SCS 121 CL. O delineamento experimental utilizado foi o de blocos casualizados com tratamentos arranjados em esquema fatorial 4 x 2, com quatro repetições. Avaliou-se dois níveis de tratamento de sementes (com ou sem) e quatro tratamentos herbicidas (sem herbicida, [imazapyr + imazapic] + bentazon, [imazapyr + imazapic] + saflufenacil e [imazapyr + imazapic] + bentazon + saflufenacil). A primeira aplicação foi realizada em V2 apenas com a mistura comercial de [imazapyr + imazapic] e a segunda aplicação foi realizada em V3 com tratamentos. As variáveis avaliadas foram fitointoxicação, número de plantas por metro quadrado, número de perfilhos por planta, número de panículas por metro quadrado, número de grãos por panícula, massa da panícula, esterilidade de espiguetas, massa de mil grãos e produtividade de grãos. Concluiu-se que o tratamento de sementes com a mistura formulada de fungicida e inseticida reduziu a fitointoxicação da mistura de [imazapyr + imazapic] + bentazon + saflufenacil. Além disso, houve superioridade numérica nos componentes de rendimento e na produtividade de grãos com o tratamento de semente. ABSTRACTIncreased phytointoxication level after herbicide tank mixtures has been frequent in field conditions, pressured by the improvement of the control spectrum and the advance of herbicide-resistant biotypes. The objective of this work was to assess the interaction of seed treatment with a commercial formulation of fungicide and insecticide with herbicide tank mixture after the application in paddy rice, in the pregerminated seed system, in the Alto Vale do Itajaí region. To carry out the experiment in the commercial field prepared for the pre-germinated seed system, sowing SCS 121 CL cultivar. The experimental design was a block completely randomized with treatments organized in factorial design 4 x 2, with four replications. We assessed two levels seed treatment (with or without) and four herbicide treatments (without herbicide, [imazapyr + imazapic] + bentazon, [imazapyr + imazapic] + saflufenacil, and [imazapyr + imazapic] + bentazon + saflufenacil). The first spray was carried out in V2 only with the commercial mixture of [imazapyr + imazapic], and the sec...

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Reactive oxygen species trigger the fast action of glufosinate

Cited by 85 publications

References 35 publications

Effect of Phosphinothricin on Transgenic Downy Birch (Betula pubescens Ehrh.) Containing bar or GS1 Genes

Effect of Phosphinothricin on Transgenic Downy Birch (Betula pubescens Ehrh.) Containing bar or GS1 Genes

Light activates the translational regulatory GCN2 kinase via reactive oxygen species emanating from the chloroplast

Interação do tratamento de sementes e da mistura em tanque de herbicidas na seletividade para o arroz irrigado

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