2005
DOI: 10.1016/j.freeradbiomed.2004.10.022
|View full text |Cite
|
Sign up to set email alerts
|

Reactivity of 2?,7?-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
137
0
5

Year Published

2007
2007
2022
2022

Publication Types

Select...
8
2

Relationship

0
10

Authors

Journals

citations
Cited by 180 publications
(142 citation statements)
references
References 43 publications
0
137
0
5
Order By: Relevance
“…After a 10-min incubation at the ambient temperature, the fluorescence intensity of the oxidation product (rhodamine 123) was measured. H 2 R 123 is known to be oxidized to the fluorescent compound rhodamine 123 by hydrogen peroxide and organic peroxides in reactions catalyzed by peroxidases or ferric ions, but also by a range of other oxidants [20,21]. Though the identification of specific oxidants of H 2 R 123 in a cell is not easy, the rate of oxidation is usually assumed to reflect the overall level of the reactive oxygen species.…”
Section: Resultsmentioning
confidence: 99%
“…After a 10-min incubation at the ambient temperature, the fluorescence intensity of the oxidation product (rhodamine 123) was measured. H 2 R 123 is known to be oxidized to the fluorescent compound rhodamine 123 by hydrogen peroxide and organic peroxides in reactions catalyzed by peroxidases or ferric ions, but also by a range of other oxidants [20,21]. Though the identification of specific oxidants of H 2 R 123 in a cell is not easy, the rate of oxidation is usually assumed to reflect the overall level of the reactive oxygen species.…”
Section: Resultsmentioning
confidence: 99%
“…Intracellular level of reactive oxygen species (ROS) was determined using the redox-sensitive probes DCFH-DA in conjunction with fluorescent microscopy (41,44,46). Briefly, neutrophils (2.5 ϫ 10 6 /well) were incubated with DCFH-DA (10 M) in a four-well chambered coverglass (Nalge, Naperville, IL) Fig.…”
Section: Methodsmentioning
confidence: 99%
“…Figures 1B and 1C). The DCFH-DA probe is not a specific indicator of H 2 O 2 because it is oxidized by reactive nitrogen species and hydroxyl radical (39,40). Because ATZ inhibits catalase but not other enzymes involved in H 2 O 2 removal, the observed increase in DCF fluorescence in ATZ-treated neutrophils is likely to reflect elevated intracellular H 2 O 2 concentrations.…”
Section: Catalase Inhibition Increases Intracellular Levels Of H 2 O mentioning
confidence: 99%