Reactivity of a Rabbit Antiserum against Highly Purified HLA‐DR Antigens

Klareskog, Lars; Trägårdh, L; Lindblom, J. B.; Peterson, Per A.

doi:10.1111/j.1365-3083.1978.tb00444.x

Cited by 71 publications

(31 citation statements)

References 26 publications

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“…If so, Ia antigens would be likely candidates to participate in the cell-to-cell interactions. With the use of a rabbit antiserum against Ia antigens [13,141 and with anti-Ia antigen alloantisera we demonstrate that epithelial cells in the lactating mammary glands of guinea pigs and mice contain Ia antigens. The Ia antigens are induced by pregnancy, and lactotropic hormones administered to virgin animals evoke the same effect.…”

Section: Introductionmentioning

confidence: 98%

Hormonal regulation of the expression of la antigens on mammary gland epithelium

1980

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Guinea pig and mouse mammary gland epithelial cells express Ia antigen-like molecules that react specifically with a rabbit anti-Ia antigen antiserum. The murine Ia antigen-like molecules were shown to share alloantigenic determinants with regular spleen cell Ia antigens. The expression of the mammary gland Ia antigens is under hormonal control, i.e. the Ia antigen expression is induced by pregnancy and lactation and can also be induced by the exogenous administration of lactotropic hormones.

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Section: Introductionmentioning

confidence: 98%

Hormonal regulation of the expression of la antigens on mammary gland epithelium

1980

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“…The rabbit antiserum against HLA-DR antigens and the IgG Fab fragments thereof have been described (12). Rabbit anti-alkaline phosphatase was provided by D. Y. Mason (13).…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

“…The culture conditions and means ofdetermining the responses were as described (4). For the antibody inhibition experiments, Fab fragments of normal rabbit serum and rabbit anti-HLA-DR antibodies were used in concentrations (0.05 mg/ml) that specifically inhibit allogeneic T-cell stimulation by peripheral blood lymphocytes (12).…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

Evidence in support of a self-perpetuating HLA-DR-dependent delayed-type cell reaction in rheumatoid arthritis.

Klareskog¹,

Forsum²,

Scheynius³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

247

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Originating from observations on similarities between the rheumatoid synovial tissue and sldn lesions in delayedtype hypersensitivity reactions-similarities as to massive infiltrates of "helper" T lymphocytes close to HLA-DR-expressing macrophage/dendritic cells-a notion is formed on the importance of local macrophage-dependent helper T-cell activation in the rheumatoidjoint similar to that in a delayed-type skdn reaction.

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“…The antisera directed against O2-microglobulin and the heavy chains of HLA-A and -B antigen were the same as described (1). The reactivity of the anti-HLA-D antisera has been detailed (9). Fab fragments were prepared from the IgG fractions of the antisera and from normal rabbit IgG (10).…”

mentioning

confidence: 99%

Binding of HLA antigen-containing liposomes to bacteria

Klareskog¹,

Banck²,

Forsgren³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Highly purified, detergent-solubilized HLA-A and -B antigens and HLA-D antigens were separately incorporated into liposomes. Deter The major histocompatibility complex (MHC) controls the expression of several types of cell surface glycoproteins that seem to have been highly conserved during evolution. In man, two types of MHC antigens have been distinguished. The HLA-A, -B, and -C antigens are composed of two types of polypeptide chains (1, 2). The larger subunit expresses the particular alloantigenic determinants and is intergrated into the hydrocarbon matrix of the membrane whereas the smaller subunit, 02-microglobulin, is invariant and associated with all known HLA-A, -B, and -C antigens (see refs. 1-4). The HLA-D antigens are also composed of two types of dissimilar polypeptide chains, which are membrane integrated (5).It is now well established that the two classes of transplantation antigen molecules participate in various types of cell-cell interactions, at least among the cells of the immune system (for a review, see ref. 6). Thus, the HLA-A and -B antigens, and their counterparts in other species, are essential in the recognition of foreign antigens (7). The HLA-D antigens and their structural equivalents in the mouse and the guinea pig, the Ta antigens, are needed on the surface of macrophages to allow a proper presentation of foreign antigens to helper T lymphocytes (8). Whether these functions for the HLA-A, -B, and -D antigens are the only ones is far from established.The structural similarity between the HLA-A and -B antigens and the immunoglobulins (3, 4) and the functional requirement for MHC antigens in the physiological activation of T lymphocytes against foreign antigens argues for the idea that the transplantation antigens in some manner can physically interact with foreign substances. To Antisera. The antisera directed against O2-microglobulin and the heavy chains of HLA-A and -B antigen were the same as described (1). The reactivity of the anti-HLA-D antisera has been detailed (9). Fab fragments were prepared from the IgG fractions of the antisera and from normal rabbit IgG (10). The reactivity of all the antisera used could completely be absorbed out with papain-solubilized transplantation antigens. Thus, these antisera did not display measurable reactivity against the intracellular or membrane-integrated portions of the transplantation antigens (unpublished observations).Bacteria. Five bacterial strains obtained as clinical isolates from the routine diagnostic laboratory were used. The strains used were Neisseria catarrhalis § 5 and 807 (NC 5

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Reactivity of a Rabbit Antiserum against Highly Purified HLA‐DR Antigens

Cited by 71 publications

References 26 publications

Hormonal regulation of the expression of la antigens on mammary gland epithelium

Hormonal regulation of the expression of la antigens on mammary gland epithelium

Evidence in support of a self-perpetuating HLA-DR-dependent delayed-type cell reaction in rheumatoid arthritis.

Binding of HLA antigen-containing liposomes to bacteria

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