We present experimental evidence suggesting that frequency and literacy predict online processing and comprehension of subject-verb agreement constructions by adult native speakers of English. We measured participants’ eye fixations, reaction times, and response accuracy in a forced-choice task using audio-visual eye-tracking paradigm. Participants completed a battery of tasks, inc. the Literacy Rating Scale (Tarone et al., Literacy and Second Language Oracy-Oxford Applied Linguistics, Oxford University Press, 2013), Agreement Judgement Task (e.g., Veenstra et al., Frontiers in Psychology 5:783, 2014). The AJT involved matching an auditorily presented subject phrase to one of two images of easily distinguishable colours presented on a computer screen (e.g., stars, circles). Participants heard 42 test sentences, counterbalanced across the three types: Type 1 (e.g., ‘The stars with the circles are blue’), Type 2 (e.g., ‘The star with the circles is blue’) and Type 3 (e.g., ‘The star with the circles are blue’*. Type 1 and Type 2 constructions are considerably more frequent in writing than in speech (Miller et al., Spontaneous spoken language: Syntax and discourse, Oxford University Press on Demand, 1998) with Type 2 producing more attraction errors (Bock et al., Cognitive Psychology 43:83–128, 2001; Becker, L., & Dąbrowska, E. (2020). Does experience with written language influence grammaticality intuitions? UK Cognitive Linguistics Conference: University of Birmingham [conference presentation].). Data were analysed with linear mixed effects models and generalised additive models. Results show lower literacy participants took longer to process sentential cues and made more attraction errors. These findings support usage-based research showing frequency and experience effects on online comprehension of canonical and non-canonical constructions (Farmer, T. A., Misyak, J. B., & Christiansen, M. H. (2012). Individual differences in sentence processing. In Cambridge handbook of psycholinguistics (pp. 353-364)., Street, Language Sciences 59:192–203, 2017), detection and production of agreement attraction errors (Becker, L., & Dąbrowska, E. (2020). Does experience with written language influence grammaticality intuitions? UK Cognitive Linguistics Conference: University of Birmingham [conference presentation].) and demonstrate how linguistic and attentional processes interact (Tomlin and Myachykov, Attention and salience, Handbook of Cognitive Linguistics, 2015). They also complement corpus-based studies by providing evidence that native speakers are sensitive to observed distributions (Miller et al., Spontaneous spoken language: Syntax and discourse, Oxford University Press on Demand, 1998).