Real surface area measurements in electrochemistry

Trasatti, Stefano P.; Петрий, О. А.

doi:10.1351/pac199163050711

Cited by 1,666 publications

(1,060 citation statements)

References 3 publications

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“…Charge required for dessorption and calculated from the reduction peak area with one electron process assumption [41] was used for the estimation of surface concentration of adsorbed disulfide after 24 hours of incubation, which was 1.7 nmols·cm -2 . Taking into account the roughness coefficient of the gold electrodes about 3.9 [41,42], the value of 0.4 nmols·cm -2 of disulfide surface concentration was calculated, which is close to theoretical value of 0.8 nmols·cm -2 for condensed monolayer [36].…”

Section: Functional Sam On Gold Surfacesupporting

confidence: 79%

Direct reagentless detection of the affinity binding of recombinant His-tagged firefly luciferase with a nickel-modified gold electrode

Vagin

Trashin

Белоглазкина

et al. 2015

Mendeleev Communications

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The direct reagentless electrochemical detection of recombinant firefly luciferase binding with gold electrode modified with nickel ions has been carried out. Self-assembly of molecules of functional disulfide containing chelating moieties on the gold electrode was monitored by contact angle measurements and confirmed by electrochemical data as three times decrease of electric double layer capacitance. Succeeding nickel ions chelating followed by selective binding of target protein were registered by reagentless electrochemical measurements as consistent decreases of double layer capacitance. The quantification of bound protein has been achieved by means of luminescent assay of residual enzymatic activity in solution for electrode recovery. The electrode surface coverage by protein globules has been confirmed by AFM.

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Section: Functional Sam On Gold Surfacesupporting

confidence: 79%

Direct reagentless detection of the affinity binding of recombinant His-tagged firefly luciferase with a nickel-modified gold electrode

Vagin

Trashin

Белоглазкина

et al. 2015

Mendeleev Communications

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“…The gold NPs of 15 nm average diameter were prepared by the procedure of Turkevich et al [41] and Frens [42]. The effective area of the electrode was estimated by integration of the Au-O reduction peak at 1.1 V in the cyclic voltammogram recorded in 0.1 M H2SO4 and taking a value of 390 μC.cm -2 for a gold oxide surface [43]. Different modifications of the gold surface were probed as follows: i) 1mM solution of mercaptopropionic acid in water, ii) cysteamine in water, iii) 6-mercaptohexanoic acid in ethanol, iv) a 1/1 mixture of 6-mercaptohexan-1-ol and 1-hexanethiol in ethanol.…”

Section: Voltammetric Experimentsmentioning

confidence: 99%

Electrochemical study of an electron shuttle diheme protein: The cytochrome c from T. thermophilus

Melin

Schoepp‐Cothenet²,

Abdulkarim³

et al. 2017

Inorganica Chimica Acta

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ABSTRACT:Cytochrome c550, a diheme protein from the thermophilic bacterium Thermus thermophilus, is involved in an alternative respiration pathway allowing the detoxification of sulfite ions. It transfers the two electrons released from the oxidation of sulfite in a sulfite:cytochrome c oxidoreductase (SOR) enzyme to heme/copper oxidases via the monoheme cytochrome c552. It consists of two conformationally independent and structurally different domains (the C-and Nterminal) connected by a flexible linker. Both domains harbor one heme moiety. We report here the redox properties of the full-length protein and the individual C-and N-terminal fragments.We show by UV/Vis and EPR potentiometric titrations that the two fragments exhibit very similar potentials, despite their different environments. In the full-length protein, however, the N-terminal heme is easier to reduce than the C-terminal one, due to cooperative interactions.This finding is consistent with the kinetic measurements which showed that the N-terminal domain only accepts electrons from the SOR. Cytochrome c552 is able to interact with its partners both through electrostatic and hydrophobic interactions as could be shown by measuring efficient electron transfer at gold electrodes modified with charged and hydrophobic groups, respectively. The coupling of electrochemistry with infrared spectroscopy allowed us to monitor the conformational changes induced by electron transfer to each heme separately and to both simultaneously.

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“…In this work, the surface area of the porous gold was determined first by integrating the reduction peak area of oxidized gold layer formed by cyclic voltammetry (CV) scans from −0.2 to +1.6 V in 0.5 M H 2 SO 4 [20,21]. The average value of the electroactive surface area of the electrode can be calculated according to the Randles-Sevcik equation [22].…”

Section: Estimation Of the Active Surface Area Of The Electrodementioning

confidence: 99%

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Ding

Zhang

Jia

et al. 2014

Sensors and Actuators B: Chemical

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a b s t r a c tAn electrochemical sensing platform based on assembly of carbon nanotubes on a nanoporous gold electrode is described for highly sensitive detection of organophosphate pesticides. The nanoporous gold film (NPG) electrode is fabricated by an alloying/dealloying process, which possess high electroactive surface area and is an excellent substrate for sensor design. The NPG functionalized with cysteamine allows the immobilization of carbon nanotubes on the electrode with the self-assembly technique. The carboxylated carbon nanotubes are further linkered with acetylcholinesterase (AChE) for amperometric sensing of pesticides. The immobilized AChE, as a model, shows excellent activity to its substrate and allows a quantitative measurement of organophosphate pesticides. Under the optimal experimental conditions, the inhibition of malathion is proportional to its concentration in the range of 0.001-0.5 g mL −1 with a detection limit of 0.5 ng mL −1 . The proposed method shows good reproducibility and high stability, which provides a new avenue for electrochemical biosensor design.

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Real surface area measurements in electrochemistry

Cited by 1,666 publications

References 3 publications

Direct reagentless detection of the affinity binding of recombinant His-tagged firefly luciferase with a nickel-modified gold electrode

Direct reagentless detection of the affinity binding of recombinant His-tagged firefly luciferase with a nickel-modified gold electrode

Electrochemical study of an electron shuttle diheme protein: The cytochrome c from T. thermophilus

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

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