Real-time assessment of cytotoxicity by impedance measurement on a 96-well plate

Ceriotti, Laura; Ponti, Jessica; Broggi, Francesca; Kob, A.; Drechsler, S.; Thedinga, Elke; Colpo, Pascal; Sabbioni, E.; Ehret, Ralf; Rossi, François

doi:10.1016/j.snb.2006.10.024

Cited by 69 publications

(58 citation statements)

References 31 publications

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“…Often, systems are described, where cells are grown on microplates or other small units with integrated electrodes, where the dielectric properties of single cells can be monitored. To assess cell adhesion and morphological changes of mouse cells, immortalized fibroblasts [Ceriotti et al, 2007a], cardiomyocytes [Qui et al, 2008], and mammalian cancer cells [Rahman et al, 2008] were monitored in a 96-well plate and in small volume detection chambers, where probes are integrated [Ceriotti et al, 2007a, Ehret et al, 1997, Ehret et al, 1998, Guo et al, 2006, Yu et al, 2006. Furthermore, the monitoring of apoptosis and necrosis , Qiu et al, 2009 as well as monitoring of cytotoxic effects of various substances by DS have been described [Ceriotti et al, 2007b, Liu et al, 2009, Malleo et al, 2009.…”

Section: Applications In Researchmentioning

confidence: 99%

Process control in cell culture technology using dielectric spectroscopy

Justice¹,

Brix

Freimark³

et al. 2011

Biotechnology Advances

107

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Section: Applications In Researchmentioning

confidence: 99%

Process control in cell culture technology using dielectric spectroscopy

Justice¹,

Brix

Freimark³

et al. 2011

Biotechnology Advances

107

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“…xCELLigence system -Real-Time Cell Analyser (RTCA) The RTCA system was described by many authors (Xing et al 2006;Ceriotti et al 2007;Slanina et al 2011;Garcia et al 2013). It utilizes a series of micro wells with bottoms covered by 80% with microelectrodes that measure cell viability by monitoring cell proliferation and morphology.…”

Section: Chemicalsmentioning

confidence: 99%

Real-time monitoring of cadmium toxicity in rabbit kidney cells

et al. 2015

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The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and proliferative activity during the whole time of experiment. Moreover, after 24 h exposure of cells to cadmium, colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyl tetrazolium bromide (MTT) test was used to measure the metabolic activity and cytotoxicity was determined by measurement of lactate dehydrogenase (LDH) leaked from damaged cells. We found that renal cells exposed to lower concentrations (5-10 mg·l -1 ) of cadmium tend to grow similarly to control cells, however, cell index was significantly different (P < 0.05) after 24 h. With increasing concentration of cadmium (15-50 mg·l -1 ) significantly lower proliferative (P < 0.05) and metabolic activity (P < 0.05) of cells was observed and cytotoxicity increased simultaneously (P < 0.001). In addition, we found that the real-time monitoring of the cell response was significantly correlated with commonly used biological methods for toxicity measurement, for MTT assay R 2 was 0.9448 (P < 0.01) and for LDH assay R 2 was 0.9466 (P < 0.01), respectively. The present study is the first report when combination of RTCA, MTT assay and LDH test was used for cadmium nephrotoxicity assessment. In all these methods, the toxic effect of cadmium on rabbit kidney cells increased in a concentration-dependent manner. xCELLigence system, nephrotoxicity, cytotoxicity, lactate dehydrogenaseIncreased industrial activities brought gradual redistribution of a number of toxic elements from the terrestrial crust into the environment and thus increased the potential exposure of humans and animals. With rising environmental pollution the interest arises in the consequences of the action of xenobiotics, including hazardous chemical elements, on live organisms (Nováková et al. 2007;Čelechovská et al. 2008). A series of trials were designed to evaluate the responses of tissues and organs of animal species of economic importance, such as the rabbit, to trace elements (cadmium, lead, mercury) at concentrations that could potentially exist in the feeds on a farm. The daily rations included carrots, potatoes, or beetroot. Cadmium belongs to heavy metals widely distributed in the environment, obtained as a by-product of refining. Its compounds are used in the electroplating of metals, alkaline batteries or in compounds with other metals. Relatively high quantities of cadmium are present in phosphate fertilisers (from some locations), which increase the concentration of cadmium in soil and plants. Cadmium is also highly toxic to organisms living in the aqueous environment (Koréneková et al. 2002). Because of its ability to reabsorb and accumulate divalent metals, kidney is the first target organ of heavy metal toxicity. The extent of renal da...

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“…Any unwanted DC voltages present in the measurement range are decoupled by means of capacitors connected to the output of the impedance analyzer [16]. Commercially available devices for impedimetric proliferation measurements operate only at fixed frequencies, typically in the 10 kHz-100 kHz range [20][21][22]. Since different cell types have different sizes and shapes the choice was made to sweep a broader spectrum so ideal measurement frequencies could be determined for each of the three cell types.…”

Section: +mentioning

confidence: 99%

Cell proliferation monitoring by multiplexed electrochemical impedance spectroscopy on microwell assays

Duchateau

Broeders

Croux

et al. 2013

Phys. Status Solidi C

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Cell proliferation can be monitored by a wide range of well‐established techniques. Most of the principles used rely on optical, single end‐point methods, often involving the use of absorbent, fluorescent or luminescent compounds. These additives can interfere with the cell growth, thus producing distorted results. Electrochemical impedance spectroscopy provides a solution for this problem and enables continuous monitoring without interference. However equipment for this measurement technique is often bulky, highly expensive and lacks multichannel features. This paper presents a low‐cost, compact hardware platform optimized for proliferation measurements, together with custom software to ease interpretation and physical modelling of data. Performance is demonstrated and measurement parameters are fine‐tuned for three commonly used cell types in proliferation measurements. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Real-time assessment of cytotoxicity by impedance measurement on a 96-well plate

Cited by 69 publications

References 31 publications

Process control in cell culture technology using dielectric spectroscopy

Process control in cell culture technology using dielectric spectroscopy

Real-time monitoring of cadmium toxicity in rabbit kidney cells

Cell proliferation monitoring by multiplexed electrochemical impedance spectroscopy on microwell assays

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