Real-time detection of allele-specific polymerase chain reaction products by automated ultra-thin-layer agarose gel electrophoresis

Ultrathin-layer sodium dodecyl sulfate gel electrophoresis of proteins: Effects of gel composition and temperature on the separation of sodium dodecyl sulfate-protein complexes

“…The quite adequate mechanical strength of agarose, even at low gel concentrations, biological inertness, and stability in the pH range of 4±9 made it a popular electrophoresis separation matrix. The relatively large temperature difference between the gelling and melting temperatures of the agarose matrix (35±40 o C vs. 70±90 o C) makes possible its use as an effective separation medium even at higher electric field strengths of >40 V/cm, especially under ultrathinlayer separation conditions, where extra Joule heat dissipation is efficient [13]. In order to increase mechanical stability and/or resolving power of agarose-based separation matrices, composite gels containing agarose mixed with other polymers, such as cross-linked polyacrylamide, have been also reported [14].…”

Prenatal Diagnosis of Steroid 21-Hydroxylase Deficiency by Allele-Specific Amplification

“…ASA in conjunction with traditional agarose gel electrophoresis [15], capillary electrophoresis [20] and ultrathin-layer gel electrophoresis [21] proved to be a useful tool for determining the mutational spectrum of the Hungarian 21-OHD population. ASA was also the method of choice for the first prenatal diagnosis of 21-OHD in Hungary based on CVS DNA analysis of an affected female fetus, whose virilization was successfully prevented by the antenatal dexamethasone treatment.…”

Human dopamine D4 receptor allele genotyping by ultrathin agarose gel electrophoresis with To-Pro-3 complexation

“…Samples originated from the previously described nested PCR system, and were diluted 7-fold. Aliquots (0.5 mL) of the reaction mixture were applied onto a membrane sample loader [23]. Note that although the separation gel contained just a fraction of the complexing agent To-Pro-3 suggested in the literature [24], the detection limit was 20±50 times better than that of attained by conventional agarose electrophoresis with ethidium bromide staining.…”