Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses

Besson-Bard, Angélique; Griveau, Sophie; Bedioui, Féthi; Wendehenne, David

doi:10.1093/jxb/ern189

Cited by 46 publications

(41 citation statements)

References 38 publications

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“…Therefore, the regulation of the expression of NAS4 might differ from those of IRT1 and FRO2. release of NO from DEANO occurs within seconds in aqueous solution 17 whereas the release form GSNO is slower. 18 Although speculative, we assume that these parameters might influence the action of NO.…”

Section: Resultsmentioning

confidence: 99%

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Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals inA. thalianaplants grown under iron deficiency

Koen

Szymańska

Klinguer

et al. 2012

Plant Signaling & Behavior

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Section: Resultsmentioning

confidence: 99%

“…GSNO and GSH were dissolved in water, DEANO was prepared as previously described. 17 As a control for DEANO effects, plants were treated with 50 μM diethylamine prepared in water.…”

Section: Methodsmentioning

confidence: 99%

Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals inA. thalianaplants grown under iron deficiency

Koen

Szymańska

Klinguer

et al. 2012

Plant Signaling & Behavior

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“…The effect of salt was studied by complementing the hydroponic solution with 100 mM NaCl over 45 min or 2 h. For mannitol treatment, plants were grown in a hydroponic solution containing 200 mM mannitol over 45 min to 6 h. Plants were treated with 500 M H 2 O 2 over 8 or 20 min. Nitric oxide (NO) treatment was applied by using diethylamine NONOate (DEA-NO), an NO donor, as previously described (24). Briefly, DEA-NO was dissolved in 0.01 M NaOH as a stock solution (500 mM).…”

Section: Methodsmentioning

confidence: 99%

“…S1B). Pre-treatment of the root system with an NO scavenger (100 M cPTIO) (24,25) fully counteracted the inhibitory effects of 100 M DEA-NO (supplemental Fig. S1C), indicating that NO specifically inhibits Arabidopsis Lp r .…”

Section: Modulation Of Lp R By Multiple Abioticmentioning

confidence: 97%

Coordinated Post-translational Responses of Aquaporins to Abiotic and Nutritional Stimuli in Arabidopsis Roots

Pietro

Vialaret

et al. 2013

Molecular & Cellular Proteomics

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In plants, aquaporins play a crucial role in regulating root water transport in response to environmental and physiological cues. Controls achieved at the post-translational level are thought to be of critical importance for regulating aquaporin function. To investigate the general molecular mechanisms involved, we performed, using the model species Arabidopsis, a comprehensive proteomic analysis of root aquaporins in a large set of physiological contexts. We identified nine physiological treatments that modulate root hydraulics in time frames of minutes (NO and H 2 O 2 treatments), hours (mannitol and NaCl treatments, exposure to darkness and reversal with sucrose, phosphate supply to phosphate-starved roots), or days (phosphate or nitrogen starvation). All treatments induced inhibition of root water transport except for sucrose supply to darkgrown plants and phosphate resupply to phosphatestarved plants, which had opposing effects. Using a robust label-free quantitative proteomic methodology, we identified 12 of 13 plasma membrane intrinsic protein (PIP) aquaporin isoforms, 4 of the 10 tonoplast intrinsic protein isoforms, and a diversity of post-translational modifications including phosphorylation, methylation, deamidation, and acetylation. A total of 55 aquaporin peptides displayed significant changes after treatments and enabled the identification of specific and as yet unknown patterns of response to stimuli. The data show that the regulation of PIP and tonoplast intrinsic protein abundance was involved in response to a few treatments (i.e. NaCl, NO, and nitrate starvation), whereas changes in the phosphorylation status of PIP aquaporins were positively correlated to changes in root hydraulic conductivity in the whole set of treatments The absorption of soil water by roots is crucial in order for plants to maintain their water status. Studies in various plant species have shown that the root water permeability (root hydraulic conductivity Lp r ) is constantly adjusted depending on the developmental stage of the plant, its nutritional or hormonal status, or multiple environmental stimuli (1). Despite their importance in plant growth and adaptation, these multiple responses have not been investigated in a single plant species yet. Aquaporins form a large class of channel proteins that facilitate the diffusion of water and small neutral solutes across cell membranes and, among many other functions, contribute to root water uptake (2, 3). Aquaporins are 25-to 30-kDa proteins with six membrane-spanning domains and five connecting loops (A-E), with N-and C-terminal tails exposed to the cytosol (4, 5). Plant aquaporins show a high multiplicity of isoforms. Thirty-five homologs belonging to four homology subclasses have been identified in Arabidopsis. The plasma membrane intrinsic proteins (PIPs) 1 (with 13 isoforms further subdivided into the PIP1 and PIP2 subgroups) and the tonoplast intrinsic proteins (TIPs) with 10 homologs are the most abundant aquaporins in the plasma membrane and the tonoplast, respectivel...

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“…Peptidoglycan, a MAMP associated with gram-positive bacteria, also induces plant immune signaling dependent on [Ca 2+ ] cyt elevation and NO generation (Gust et al, 2007). The fungal MAMPs xylanase and chitosan also cause NO generation in plants (Laxalt et al, 2007;Raho et al, 2011), as do the Phytophthora infestans oomycete elicitins (INF1; Asai et al, 2008) and cryptogein (Foissner et al, 2000;Lamotte et al, 2004;Besson-Bard et al, 2008b). One of the best-characterized MAMPs, in terms of plant immune responses, is the bacterial motility organ protein flagellin (flg).…”

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confidence: 99%

There's More to the Picture Than Meets the Eye: Nitric Oxide Cross Talk with Ca2+ Signaling

et al. 2013

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Calcium and nitric oxide (NO) are two important biological messengers. Increasing evidence indicates that Ca 2+ and NO work together in mediating responses to pathogenic microorganisms and microbe-associated molecular patterns. Ca 2+ fluxes were recognized to account for NO production, whereas evidence gathered from a number of studies highlights that NO is one of the key messengers mediating Ca 2+ signaling. Here, we present a concise description of the current understanding of the molecular mechanisms underlying the cross talk between Ca 2+ and NO in plant cells exposed to biotic stress. Particular attention will be given to the involvement of cyclic nucleotide-gated ion channels and Ca 2+ sensors. Notably, we provide new evidence that calmodulin might be regulated at the posttranslational level by NO through S-nitrosylation. Furthermore, we report original transcriptomic data showing that NO produced in response to oligogalacturonide regulates the expression of genes related to Ca 2+ signaling. Deeper insight into the molecules involved in the interplay between Ca 2+ and NO not only permits a better characterization of the Ca 2+ signaling system but also allows us to further understand how plants respond to pathogen attack.

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Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses

Cited by 46 publications

References 38 publications

Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals inA. thalianaplants grown under iron deficiency

Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals inA. thalianaplants grown under iron deficiency

Coordinated Post-translational Responses of Aquaporins to Abiotic and Nutritional Stimuli in Arabidopsis Roots

There's More to the Picture Than Meets the Eye: Nitric Oxide Cross Talk with Ca2+ Signaling

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