Real-time fluorescent determination and biological imaging in living models via a butyrylcholinesterase-activated fluorescent probe

Development of Cholinesterase-Activatable Fluorescent Probes for Pesticide Residue Detection

“…To address this issue, a fluorescent probe-based assay has emerged as a promising alternative for sensing and imaging cholinesterases, due to its high sensitivity and selectivity, simple operation, fast response speed, and good stability. − Some excellent works about the detection of the AChE − and BChE − and the discovery of cholinesterase inhibitors − have been reported. However, designing specific probes for distinguishing between AChE and BChE is very challenging.…”

Real-time visualization of butyrylcholinesterase activity using a highly selective and sensitive chemiluminescent probe

“…For instance, elevated expression of tumor associated BChE has been shown to be related to the development of neuroblastoma, demonstrating its potential as a tumor biomarker. [29][30][31][32][33][34][35][36][37][38] Considering broad contributions of BChE to physiological and pathological processes, it is of great importance to monitor BChE activity and its spatial/temporal distributions in living cells with a high selectivity and sensitivity.…”

Pseudo-irreversible butyrylcholinesterase inhibitors: Structure–activity relationships, computational and crystallographic study of the N-dialkyl O-arylcarbamate warhead

“…Moreover, AChE and BChE can be used as biomarkers for detection of Alzheimer's disease [1,31,32], Hirschsprung's disease [33,34] and multiple sclerosis [35,36]. To date, only a few AChE [37][38][39][40][41][42] and BChE [43][44][45][45][46][47] fluorescent probes and histochemical methods [48] have enabled tissue ChE location and activity determination. Accordingly, the development of new chemical probes for investigating the roles of ChEs in physiological and pathophysiological processes in vitro and ex vivo is required by biologists and neurobiologists [49][50][51].…”