Real-time visualization of butyrylcholinesterase activity using a highly selective and sensitive chemiluminescent probe

Abstract

Butyrylcholinesterase (BChE), one of the critical human cholinesterases, plays crucial roles in numerous physiological and pathological processes. Accordingly, it is a striking and at the same time challenging target for...

Rechargeable Afterglow Nanotorches for In Vivo Tracing of Cell‐Based Microrobots

“…Unfortunately, the further clinical application of iPLNPs was greatly restricted due to the potential risk of the leakage of heavy metal ion from these inorganic nanoparticles [13–14] . On the contrast, organic persistent luminescence nanoparticles (oPLNPs) containing organic chemiluminescence agents [15] (such as peroxalate esters, [16] Schaap's 1,2‐dioxetanes including new generation ones, [17–22] etc) and organic photosensitizers (TPE‐TCy‐VP, [23] TPE‐Ph‐DCM, [24] PFODBT, [25] etc) showed great potential to develop biocompatible afterglow imaging probes due to their facile processability, and flexible surface functionalization [26–28] . Unfortunately, most of the oPLNPs could only be pre‐excited in vitro by visible light, which could not be re‐excited after they got into the bodies [24] .…”

Rechargeable Afterglow Nanotorches for In Vivo Tracing of Cell‐Based Microrobots

“…Unfortunately, the further clinical application of iPLNPs was greatly restricted due to the potential risk of the leakage of heavy metal ion from these inorganic nanoparticles [13–14] . On the contrast, organic persistent luminescence nanoparticles (oPLNPs) containing organic chemiluminescence agents [15] (such as peroxalate esters, [16] Schaap's 1,2‐dioxetanes including new generation ones, [17–22] etc) and organic photosensitizers (TPE‐TCy‐VP, [23] TPE‐Ph‐DCM, [24] PFODBT, [25] etc) showed great potential to develop biocompatible afterglow imaging probes due to their facile processability, and flexible surface functionalization [26–28] . Unfortunately, most of the oPLNPs could only be pre‐excited in vitro by visible light, which could not be re‐excited after they got into the bodies [24] .…”

Optical imaging probes for selective detection of butyrylcholinesterase

“…25). 141 The BChE-induced hydrolysis of the cyclopropyl based masking unit followed by the chemically initiated electron exchange luminescence (CIEEL) mechanism upon formation of phenolate ions, which chemically excited benzoate products were liberated in a consecutive manner to generate chemiluminescence signal (Fig. 25).…”