Real-Time In Situ Screening of Omega-7 Phospholipids in Marine Biological Resources Using an iKnife-Rapid-Evaporative-Ionization-Mass-Spectrometry-Based Lipidomics Phenotype

Abstract

Omega-7 (n-7) phospholipids were bioactive substances in marine animals. In this study, a fast lipidomics phenotyping approach for real-time in situ screening of n-7 phospholipids in five kinds of economic seafood, salmon, prawn, bluefin tuna, hairtail, and butterfish, was established using iKnife rapid evaporative ionization mass spectrometry (REIMS). The n-7 phospholipids were structurally characterized and quantitatively analyzed, and the profiles were statistically analyzed by multivariate recognition analysis. It indicated that the difference of n-7 phospholipids in seafood samples was significant (p < 0.05), with R 2(cum) and Q 2(cum) values of >0.9. The proportion of n-7 phospholipids in salmon was the highest (20.43%), followed by bluefin tuna, prawn, hairtail, and butterfish. The ions of m/z 742.54 (PC 16:1–18:1), 768.55 (PC 16:1–20:2), 697.48 (PE 16:1–18:1), and 699.48 (PE 16:1–18:0) were the main n-7 phospholipids. The effectiveness of iKnife REIMS was further verified by hydrophilic interaction chromatography mass spectrometry and gas chromatography. The results demonstrated that proposed iKnife REIMS was an excellent technique for front-line screening of n-7 phospholipids in a large variety of marine biological resources.

In Situ Quality Assessment of Dried Sea Cucumber (Stichopus japonicus) Oxidation Characteristics during Storage by iKnife Rapid Evaporative Ionization Mass Spectrometry

“…Generally, the VIP > 1 is viewed as a criterion for predominant variables. 45 As shown in Figure 4E, a total of seven individual phospholipid ions (m/z 721.5, m/z 739.5, m/z 831.5, m/z A Circos map is an extremely visualizing and useful tool for analyzing the correlation between the samples and variables by a holistic and integrated view in a circular plot using an intuitive and aesthetic way, and the connection strength was expressed by the line thickness. 46 Figure 5A shows that the strong correlation between the six DSC samples and the potential markers mainly including m/z 847.6 (Y) and m/z 859.6 (Z) corresponded to the thickness of lines.…”

Effect of steam explosion pretreatment on the composition and bioactive characteristic of phenolic compounds in Chrysanthemum morifolium Ramat cv. Hangbaiju powder with various sieve fractions

“…In addition, variable importance on projection (VIP) analysis is conducted by the algorithms in the PLS‐toolbox, which can reveal the significance of the variable for separating different samples. The variables with the value of VIP >1 were viewed as the criterion for significant variables (Song, Wang, et al., 2021 ). As shown in Figure 6b , three flavone glycosides (apigenin‐7‐O‐glucoside, luteolin‐7‐O‐glucoside, and linarin) were the potential phenolic compounds with VIP value >1 among three samples.…”

Degradation kinetics and isomerization of 5-O-caffeoylquinic acid under ultrasound: Influence of epigallocatechin gallate and vitamin C

“…The Kinetic parameters of degradation of 5-CQA at different pH with and without EGCG were obtained as the original material for multivariate statistical analysis by building distinct statistical models. PCA is an unsupervised algorithm, which is generally applied for dimension reduction by finding out principal components (PCs) to maximize the variance and ignoring class labels in a dataset ( Song et al, 2021 ). The PCA model was firstly carried out for exploring major trends on score plot ( Fig.…”