Real-time imaging of alkaline phosphatase activity of diabetes in mice <i>via</i> a near-infrared fluorescent probe

Wang, Wenxin; Jiang, Wenli; Guo, Hong Xia; Li, Yongfei; Li, Chunyan

doi:10.1039/d0cc07292c

Cited by 46 publications

(18 citation statements)

References 43 publications

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“…Furthermore, in the range of 1.0–40.0 μM, there is a superior linear relationship between the fluorescence intensity and H 2 S concentration (Figure C). The limit of detection (3σ/k) for H 2 S is 0.25 μM through experiments and calculations . These results illustrate that DCP-H 2 S can be employed in detecting H 2 S with high sensitivity.…”

Section: Resultsmentioning

confidence: 67%

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Near-Infrared Fluorescent Probe with Large Stokes Shift for Imaging of Hydrogen Sulfide in Tumor-Bearing Mice

et al. 2022

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Hydrogen sulfide (H2S) is an important endogenous gas signal molecule in living system, which participates in a variety of physiological processes. Very recent evidence has accumulated to show that endogenous H2S is closely associated with various cancers and can be regarded as a biomarker of cancer. Herein, we have constructed a new near-infrared fluorescent probe (DCP-H2S) based on isophorone-xanthene dye for sensing hydrogen sulfide (H2S). The probe shows remarkable NIR turn-on signal at 770 nm with a large Stokes shift of 200 nm, together with high sensitivity (15-fold) and rapid detection ability for H2S (4 min). The probe also possesses excellent selectivity for H2S over various other analytes including biothiols containing sulfhydryl (−SH). Moreover, DCP-H2S has been successfully applied to visualize endogenous and exogenous H2S in living cells (293T, Caco-2 and CT-26 cells). In particular, the excellent ability of DCP-H2S to distinguish normal mice and tumor mice is shown, and it is expected to be a powerful tool for detection of H2S in cancer diagnosis.

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Section: Resultsmentioning

confidence: 67%

“…The limit of detection (3σ/k) for H 2 S is 0.25 μM through experiments and calculations. 43 These results illustrate that DCP-H 2 S can be employed in detecting H 2 S with high sensitivity.…”

Section: ■ Results and Discussionmentioning

confidence: 70%

Near-Infrared Fluorescent Probe with Large Stokes Shift for Imaging of Hydrogen Sulfide in Tumor-Bearing Mice

et al. 2022

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“…In 2020, Li et al fabricated a fluorescent probe (29) with NIR emission and excellent water solubility for testing the activity of alkaline phosphatase (ALP) [119]. The fluorescence of the free probe 29 was interdicted by the phosphate group due to the suppressed ICT effect (Figure 24A).…”

Section: Probes Targeting Enzymesmentioning

confidence: 99%

Recent Progress in Fluorescent Probes for Diabetes Visualization and Drug Therapy

Jia

Niu

2022

Chemosensors

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Diabetes has become one of the most prevalent endocrine and metabolic diseases that threaten human health, and it is accompanied by serious complications. Therefore, it is vital and pressing to develop novel strategies or tools for prewarning and therapy of diabetes and its complications. Fluorescent probes have been widely applied in the detection of diabetes due to the fact of their attractive advantages. In this report, we comprehensively summarize the recent progress and development of fluorescent probes in detecting the changes in the various biomolecules in diabetes and its complications. We also discuss the design of fluorescent probes for monitoring diabetes in detail. We expect this review will provide new ideas for the development of fluorescent probes suitable for the prewarning and therapy of diabetes in future clinical transformation and application.

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“…However, its activity is remarkably increased in several human diseases ( Orimo, 2010 ). Despite abundant research on ALP fluorescent probes ( Supplementary Table S1 , ESI) ( Li et al, 2012 ; Zhou et al, 2016 ; Li et al, 2017 ; Liu et al, 2017 ; Liu et al, 2018 ; Zhang P. et al, 2019 ; Zhang Q. et al, 2019 ; Gao et al, 2019 ; Wang et al, 2021b ), its role in AKI remains unclear due to the lack of a kidney-specific molecular probe. Furthermore, existing ALP fluorescent molecular probes are not suitable for testing and imaging of ALP in the AKI model.…”

Section: Introductionmentioning

confidence: 99%

In situ detection of alkaline phosphatase in a cisplatin-induced acute kidney injury model with a fluorescent/photoacoustic bimodal molecular probe

Chen

Yuwen

Zhao

et al. 2022

Front. Bioeng. Biotechnol.

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Kidneys play an important part in drug metabolism and excretion. High local concentration of drugs or drug allergies often cause acute kidney injury (AKI). Identification of effective biomarkers of initial stage AKI and constructing activable molecular probes with excellent detection properties for early evaluation of AKI are necessary, yet remain significant challenges. Alkaline phosphatase (ALP), a key hydrolyzing protease, exists in the epithelial cells of the kidney and is discharged into the urine following kidney injury. However, no studies have revealed its level in drug-induced AKI. Existing ALP fluorescent molecular probes are not suitable for testing and imaging of ALP in the AKI model. Drug-induced AKI is accompanied by oxidative stress, and many studies have indicated that a large increase in reactive oxygen species (ROS) occur in the AKI model. Thus, the probe used for imaging of AKI must be chemically stable in the presence of ROS. However, most existing near-infrared fluorescent (NIRF) ALP probes are not stable in the presence of ROS in the AKI model. Hence, we built a chemically stable molecular sensor (CS-ALP) to map ALP level in cisplatin-induced AKI. This novel probe is not destroyed by ROS generated in the AKI model, thus allowing high-fidelity imaging. In the presence of ALP, the CS-ALP probe generates a new absorbance peak at 685 nm and a fluorescent emission peak at 716 nm that could be used to “turn on” photoacoustic (PA) and NIRF imaging of ALP in AKI. Levels of CS-ALP build up rapidly in the kidney, and CS-ALP has been successfully applied in NIRF/PA bimodal in vivo imaging. Through the NIRF/PA bimodal imaging results, we demonstrate that upregulated expression of ALP occurs in the early stages of AKI and continues with injury progression.

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Real-time imaging of alkaline phosphatase activity of diabetes in mice via a near-infrared fluorescent probe

Abstract: A novel water-soluble near-infrared fluorescent probe named QX-P with simple synthesis is developed for detecting ALP. The probe can not only visualize ALP activity in four cell lines, but also real-time image ALP activity of diabetes in mice.

Cited by 46 publications

References 43 publications

Near-Infrared Fluorescent Probe with Large Stokes Shift for Imaging of Hydrogen Sulfide in Tumor-Bearing Mice

Near-Infrared Fluorescent Probe with Large Stokes Shift for Imaging of Hydrogen Sulfide in Tumor-Bearing Mice

Recent Progress in Fluorescent Probes for Diabetes Visualization and Drug Therapy

In situ detection of alkaline phosphatase in a cisplatin-induced acute kidney injury model with a fluorescent/photoacoustic bimodal molecular probe

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