Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography

Taruttis, Adrian; Herzog, Eva; Razansky, Daniel; Ntziachristos, Vasilis

doi:10.1364/oe.18.019592

Cited by 147 publications

(121 citation statements)

References 32 publications

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“…This imaging method is versatile, being able to provide multifaceted information: anatomical imaging based on endogenous tissue absorption, functional imaging of blood oxygenation, as well as molecular imaging based on absorption of exogenous contrast agents. Thus, it has been widely used in various applications, including imaging of the brain, [4][5][6] cancer, 7,8 cardiovasculature, 9,10 as well as the evaluation of gene delivery efficiency.…”

Section: Introductionmentioning

confidence: 99%

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Balasundaram¹,

Ho²,

Li³

et al. 2015

IJN

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Conjugated polymers (CPs) are upcoming optical contrast agents in view of their unique optical properties and versatile synthetic chemistry. Biofunctionalization of these polymer-based nanoparticles enables molecular imaging of biological processes. In this work, we propose the concept of using a biofunctionalized CP for noninvasive photoacoustic (PA) molecular imaging of breast cancer. In particular, after verifying the PA activity of a CP nanoparticle (CP dots) in phantoms and the targeting efficacy of a folate-functionalized version of the same (folate-CP dots) in vitro, we systemically administered the probe into a folate receptor-positive (FR+ve) MCF-7 breast cancer xenograft model to demonstrate the possible application of folate-CP dots for imaging FR+ve breast cancers in comparison to CP dots with no folate moieties. We observed a strong PA signal at the tumor site of folate-CP dots-administered mice as early as 1 hour after administration as a result of the active targeting of the folate-CP dots to the FR+ve tumor cells but a weak PA signal at the tumor site of CP-dots-administered mice as a result of the passive accumulation of the probe by enhanced permeability and retention effect. We also observed that folate-CP dots produced ~4-fold enhancement in the PA signal in the tumor, when compared to CP dots. These observations demonstrate the great potential of this active-targeting CP to be used as a contrast agent for molecular PA diagnostic imaging in various biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Balasundaram¹,

Ho²,

Li³

et al. 2015

IJN

Self Cite

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“…PA imaging, however, provides a high ultrasonic spatial resolution for deep imaging by utilizing ultrasonic detection of the PA wave generated by absorbed diffuse light. 2,3 Deep PA imaging has been used to image both biological structure (e.g., internal organs, 4,5 and sentinel lymph nodes 6 ) and function (e.g., tumor hypoxia, 7 and brain oxygenation 8,9 ). The ability of PA imaging (PA microscopy and PA computed tomography 1 ) systems to render three-dimensional (3-D) volumetric images relies on recording the PA time-of-flight signals on a two-dimensional (2-D) surface facing the photoacoustic source, light-absorbing chromophores.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the system cost and complexity when employing a large number of data acquisition (DAQ) channels, currently most PA imaging systems utilize a single-element ultrasound (US) transducer [7][8][9][10] or a one-dimensional (1-D) array US probe. [4][5][6]11 Mechanical scanning of the US probe required by these systems to form 3-D PA images limits the volumetric imaging frame rate. Thus, 3-D PA imaging systems using 2-D array US probes have been recently studied.…”

Section: Introductionmentioning

confidence: 99%

In vivo three-dimensional photoacoustic imaging based on a clinical matrix array ultrasound probe

et al. 2012

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Abstract. We present an integrated photoacoustic and ultrasonic three-dimensional (3-D) volumetric imaging system based on a two-dimensional (2-D) matrix array ultrasound probe. A wavelength-tunable dye laser pumped by a Q-switched Nd:YAG laser serves as the light source and a modified commercial ultrasound imaging system (iU22, Philips Healthcare) with a 2-D array transducer (X7-2, Philips Healthcare) detects both the pulse-echo ultrasound and photoacoustic signals. A multichannel data acquisition system acquires the RF channel data. The imaging system enables rendering of co-registered 3-D ultrasound and photoacoustic images without mechanical scanning. The resolution along the azimuth, elevation, and axial direction are measured to be 0.69, 0.90 and 0.84 mm for photoacoustic imaging. In vivo 3-D photoacoustic mapping of the sentinel lymph node was demonstrated in a rat model using methylene blue dye. These results highlight the clinical potential of 3-D PA imaging for identification of sentinel lymph nodes for cancer staging in humans.

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“…18 Several groups have recently explored the potential of AUNR as exogenous contrast agents for photoacoustic imaging. [19][20][21] The current study aims to extend these uses of AUNR to include the selective labeling of atherosclerotic plaque, enabling subsequent detection using combined IVUS/ IVPA imaging.…”

Section: Introductionmentioning

confidence: 99%

Intravascular photoacoustic imaging of exogenously labeled atherosclerotic plaque through luminal blood

et al. 2012

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Abstract. Combined intravascular ultrasound and intravascular photoacoustic (IVUS/IVPA) imaging has been previously established as a viable means for assessing atherosclerotic plaque morphological and compositional characteristics using both endogenous and exogenous contrast. In this study, IVUS/IVPA imaging of atherosclerotic rabbit aortas following systemic injection of gold nanorods (AUNRs) with peak absorbance within the tissue optical window is performed. Ex vivo imaging results reveal a high photoacoustic signal from localized AUNRs in regions with atherosclerotic plaques. Corresponding histological staining further confirms the preferential extravasation of AUNRs in atherosclerotic regions with compromised luminal endothelium and acute inflammation. The ability to detect AUNRs using combined IVUS and photoacoustic imaging in the presence of luminal saline and luminal blood is evaluated using both spectroscopic and single wavelength IVPA imaging techniques. Results demonstrate that AUNR detection within the arterial wall can be achieved using both methods, even in the case of imaging through luminal blood.

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Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography

Cited by 147 publications

References 32 publications

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

In vivo three-dimensional photoacoustic imaging based on a clinical matrix array ultrasound probe

Intravascular photoacoustic imaging of exogenously labeled atherosclerotic plaque through luminal blood

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