Real-time imaging of ion uptake from root to above-ground part of the plant using conventional beta-ray emitters

Nakanishi, Teruyuki; Yamawaki, Masato; Kannno, S.; Nihei, Naoto; Masuda, Shiro; Tanoi, Keitaro

doi:10.1007/s10967-009-0343-9

Cited by 18 publications

(13 citation statements)

References 8 publications

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“…[6][7][8][9][10][11] Real-time radioisotope imaging system (RRIS), previously developed by us, can detect many types of radioisotopes, allowing us to observe the movement of various elements. 6 14,15) and the whole body of plants with larger dimensions have not been tested. Plants are supposed to have absorption and transportation dynamics unique to their growth stages; therefore, it is necessary to build a new system to observe the whole plant body.…”

Section: Introductionmentioning

confidence: 99%

Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

et al. 2019

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We updated our imaging system, namely real-time radioisotope imaging system (RRIS) to observe 32 P movement in large plants using a large size scintillator. In the previous version of RRIS, the view was limited by the size of a CsI (Tl) scintillator deposited on a fiber optic plate (FOS). However, owing to the high price and difficulty in handling of FOS, the view area was limited to 100×200 mm. In this study, we evaluated 5 large and low-cost plastic scintillators and showed that plastic scintillators can be used in RRIS for the quantitative analysis of 32 P. We also evaluated the resolution of the imaging system with plastic scintillator.

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Section: Introductionmentioning

confidence: 99%

Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

et al. 2019

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“…Development of radioisotope imaging (RI) systems [61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79][80] Two types of the real-time RI imaging system, namely macroscopic and microscopic, were developed. Both systems are designed to use conventional RI so that it is possible to perform imaging in our laboratory and not at special laboratory with special facilities.…”

Section: Roots Imbedded In Soilmentioning

confidence: 99%

Research with radiation and radioisotopes to better understand plant physiology and agricultural consequences of radioactive contamination from the Fukushima Daiichi nuclear accident

Nakanishi

2017

J Radioanal Nucl Chem

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Research carried out by me and my group over the last almost four decades are summarized here. The main emphasis of my work was and continues to be on plant physiology using radiation and radioisotopes. Plants live on water and inorganic elements. In the case of water, we developed neutron imaging methods and produced 15 O-labeled water (half-life 2 min) and applied them to understand water circulation pattern in the plant. In the case of elements, we developed neutron activation analysis methods to analyze a large number of plant tissues to follow element specific distribution. Then, we developed realtime imaging system using conventional radioisotopes for the macroscopic and microscopic observation of element movement. After the accident in Fukushima Daiichi nuclear power plant, we, the academic staff of Graduate School, have been studying agricultural effects of radioactive fallout; the main results are summarized in two books published by Springer.

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“…We have developed a live imaging system to analyze plants over a long period with conventional radionuclides, called real-time radioisotope imaging system (RRIS) (Nakanishi et al 2009). The RRIS can be applied to many kinds of nuclides available on the market because beta-rays and soft X-rays can be detected and the dynamic range of detection is about 1000 times (Sugita et al 2014); 137 Cs, a betaray emitter, may be analyzed using this system.…”

Section: Real-time Radioisotope Imaging Systemmentioning

confidence: 99%

Imaging Techniques for Radiocesium in Soil and Plants

Sugita

Hirose

Kobayashi

et al. 2016

Agricultural Implications of the Fukushima Nuclear Accident

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Various radioisotope imaging techniques have been used at the Graduate School of Agricultural and Life Sciences, University of Tokyo, to analyze samples containing radiocesium ( 137 Cs and 134 Cs). There are two types of samples: (1) environmental samples contaminated by the fallout from the Fukushima Daiichi nuclear power plant accident, which contain relatively low concentrations of radiocesium and (2) laboratory samples from tracer experiments conducted at the radioisotope institution containing relatively high concentrations of 137 Cs. The first technique used to visualize radiocesium in soil and plants was radioluminography (RLG). RLG, which makes use of an imaging plate, has a dynamic range that is large enough to detect both environmental and tracer-added samples. To quantify radiocesium distributions, the samples were frozen and sliced before contact with the imaging plate. This freezing procedure after sampling is for preventing radiocesium movement during slicing and measurement of 137 Cs distribution. After slicing, two detection methods were employed: RLG and microautoradiography (MAR). MAR is the conventional and older method for imaging radioisotopes based on the daguerreotype process. We applied this method to frozen sections and obtained 137 Cs distributions at a higher resolution than with RLG. Following this, we employed a non-destructive method for imaging 137 Cs movement in a living plant. We developed the visualization technique called real-time radioisotope imaging system and then demonstrated 137 Cs movement from soil to rice plants using a chamber containing paddy soil, water, and rice plants. Lastly, 42 K obtained by 42 Ar-42 K generation enabled a comparison between the movement of 137 Cs and 42 K. The mechanism of Cs transport has been reported to have some relationship with the K transport system, so experiments using both 137 Cs and 42 K would be useful for clarifying the mechanism in more detail.

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Real-time imaging of ion uptake from root to above-ground part of the plant using conventional beta-ray emitters

Cited by 18 publications

References 8 publications

Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

Research with radiation and radioisotopes to better understand plant physiology and agricultural consequences of radioactive contamination from the Fukushima Daiichi nuclear accident

Imaging Techniques for Radiocesium in Soil and Plants

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