Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor

Mayorga‐Martinez, Carmen C.; Treo, Ernesto Federico; Madrid, Rossana E.; Felice, C.J.

doi:10.1016/j.bios.2011.08.018

Cited by 16 publications

(6 citation statements)

References 16 publications

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“…In this principle, the mediator is reduced by the electron generated from the enzymatic reaction, and then is finally oxidized at the electrode, resulting in electron transfer to the electrode. Although the second-generation biosensor is oxygen-independent, it suffers from mediator leaching and interference, due to redox mediator selectivity [18,19,21]. Hence, the third-generation biosensor was developed to solve that issue.…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Immobilized Enzymes in Biosensor Applications

et al. 2019

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Enzyme-based biosensing devices have been extensively developed over the last few decades, and have proven to be innovative techniques in the qualitative and quantitative analysis of a variety of target substrates over a wide range of applications. Distinct advantages that enzyme-based biosensors provide, such as high sensitivity and specificity, portability, cost-effectiveness, and the possibilities for miniaturization and point-of-care diagnostic testing make them more and more attractive for research focused on clinical analysis, food safety control, or disease monitoring purposes. Therefore, this review article investigates the operating principle of enzymatic biosensors utilizing electrochemical, optical, thermistor, and piezoelectric measurement techniques and their applications in the literature, as well as approaches in improving the use of enzymes for biosensors.

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Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Immobilized Enzymes in Biosensor Applications

et al. 2019

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“…Then electrode was investigated by EIS by using human serum standard solution, which was commercially obtained with different glucose concentrations. Chronoimpedance process was carried out by dropping 50 μL serum samples on electrode and performed under 100 Hz fixed frequency by applying 0.9 V DC, 0.05 V AC potential for 200 seconds by altering previous method . Real human serum samples were used for glucose sensor performances as; reproducibility, regeneration potential, repeatability, recovery and reusability.…”

Section: Methodsmentioning

confidence: 99%

A Novel Chronoimpedimetric Glucose Sensor in Real Blood Samples Modified by Glucose‐imprinted Pyrrole‐Aminophenylboronic Acid Modified Screen Printed Electrode

Uygun

2019

Electroanalysis

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In this study, a molecularly imprinted sensor technology is engineered to detect glucose in real blood samples by chronoimpedimetrically. The imprinting process of glucose (Glc) was carried out by electrochemical polymerization of aminophenylboronic acid (APBA) and pyrrole (Py) by performing cyclic voltammetry (CV). Afterwards, glucose molecule was removed from imprinted surface by 5 % acetic acid to reveal glucose imprinted cavities. Electrochemical Impedance Spectroscopy (EIS) was used to characterize sensor modification steps and glucose removal. Glucose monitoring process was carried out chronoimpedimetrically(CI) for the first time in real blood samples. Calibration curve was prepared between 20–800 mg/dL. The standard deviations of the 18 calibration curves R2 were calculated as 0.9866±0.0066 to assess reproducibility. Recovery was calculated by using 105 mg/dL Glc Serum Sample, which was monitored by auto analyzer and into this sample 50 mg/dL Glc added and our sensor response was 147.92±2.43 mg/dL, 98.6±1.62 % (n=5). Non‐imprinted (NIP) sensor gave no signal for the glucose concentration.

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“…In addition, the inclusion of an electroactive dielectric enables the second-generation biosensor to operate at low voltages and also avoids interference from coexisting electroactive substances. Although the second-generation biosensor is oxygenindependent, it is still subject to leaching and interference from the medium due to redox medium selectivity [30]. and Prussian blue [29], which first react with the enzyme active site and then react with the electrode surface, thereby transferring electrons to generate a current signal proportional to the detected analyte concentration.…”

Section: Principle Of Enzyme Electrochemical Biosensor Constructionmentioning

confidence: 99%

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

Sun,

Wei,

Zhang

et al. 2023

Biosensors

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Electrochemical biosensors based on immobilized enzymes are among the most popular and commercially successful biosensors. The literature in this field suggests that modification of electrodes with nanomaterials is an excellent method for enzyme immobilization, which can greatly improve the stability and sensitivity of the sensor. However, the poor stability, weak reproducibility, and limited lifetime of the enzyme itself still limit the requirements for the development of enzyme electrochemical biosensors for food production process monitoring. Therefore, constructing sensing technologies based on enzyme electrochemical biosensors remains a great challenge. This article outlines the construction principles of four generations of enzyme electrochemical biosensors and discusses the applications of single-enzyme systems, multi-enzyme systems, and nano-enzyme systems developed based on these principles. The article further describes methods to improve enzyme immobilization by combining different types of nanomaterials such as metals and their oxides, graphene-related materials, metal–organic frameworks, carbon nanotubes, and conducting polymers. In addition, the article highlights the challenges and future trends of enzyme electrochemical biosensors, providing theoretical support and future perspectives for further research and development of high-performance enzyme chemical biosensors.

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Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor

Cited by 16 publications

References 16 publications

Immobilized Enzymes in Biosensor Applications

Immobilized Enzymes in Biosensor Applications

A Novel Chronoimpedimetric Glucose Sensor in Real Blood Samples Modified by Glucose‐imprinted Pyrrole‐Aminophenylboronic Acid Modified Screen Printed Electrode

Immobilization of Enzyme Electrochemical Biosensors and Their Application to Food Bioprocess Monitoring

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