Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing

Hu, Le; Wang, Yuxin; Liu, Qing; Liu, Man; Fu, Yang; Wang, Chunxiao; Pan, Panpan; Wang, Lin; Chen, Li; Chen, Jingdi

doi:10.1016/j.cclet.2023.108262

Cited by 28 publications

(9 citation statements)

References 26 publications

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“…Even so, how to topically apply these exosomes on wounds still deserves further exploration. Commonly, exosomes were embedded with various hydrogels or dressings as scaffolds to accumulate exosomes on diabetic wounds to synergistically promote wound healing [32,[64][65][66][67]. Therefore, to employ PA-ELNs in DM wound clinically, much more researches are required to determine the production routes of PA-ELNs for a large-scale production and design the appropriate scaffolds for local application.…”

Section: Discussionmentioning

confidence: 99%

Natural exosome-like nanoparticles derived from ancient medicinal insect Periplaneta americana L. as a novel diabetic wound healing accelerator

Liao

Pang

et al. 2023

J Nanobiotechnol

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Along with the recognized therapeutic outcomes of regenerative medicine, extracellular vesicles and their exosome subsets have become an alternative option for wound healing. Periplaneta americana L. (PA), an ancient and traditional medicinal insect, has been around for 300 million years, and displays magic formidable vitality and environmental adaptive ability. The linkage between intrinsic amputation regeneration feature and the acknowledged wound healing medicinal benefit of PA has never been revealed. Herein, inspired by the ability of exosomes to participate in the interkingdom communication, we explored whether this effect was ascribed to PA derived exosome-like nanoparticles (PA-ELNs). PA-ELNs were extracted by differential velocity centrifugation approach and characterized by DLS, NTA and TEM. Their cargoes were analyzed by LC-MS/MS proteomics and small RNA-seq analysis. The wound healing activity was verified in vivo and in vitro. PA-ELNs with a concentration of 2.33x109±6.35x107 particles/mL exhibited a lipid bilayer-bound membrane structure with an average size of 104.7 nm. Furthermore, the miRNA cargoes in PA-ELNs participate in some wound healing related signal pathways such as TGF-beta, mTOR, and autophagy. As expected, the in vitro tests indicated that PA-ELNs were apt to be internalized in HUVECs, L929 and RAW 264.7 cells and contributed to cell proliferation and migration. Most importantly, we demonstrated that the topical administration of PA-ELNs could remarkably accelerate wound healing in a diabetic mouse model, and was involved in anti-inflammatory, re-epithelialization and autophagy regulation. This study provides clear evidence for the first time that PA-ELNs, as diabetic wound healing accelerators, are the “bioactive code” of this ancient medicinal insect. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Natural exosome-like nanoparticles derived from ancient medicinal insect Periplaneta americana L. as a novel diabetic wound healing accelerator

Liao

Pang

et al. 2023

J Nanobiotechnol

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“…55 For instance, Hu et al have devised a hydrogel constructed through a two-step process of physical cross-linking, as illustrated in Figure 2A. 58 Initially, xanthan gum (XG) and poly(vinyl alcohol) (PVA) undergo physical cross-linking via freeze−thaw cycles, utilizing hydrogen bonds and crystalline regions to establish the initial flexible network. Subsequently, XG undergoes additional physical cross-linking by immersion in a solution containing iron ions.…”

Section: ■ Fabrication Strategiesmentioning

confidence: 99%

“…Furthermore, the incorporation of other components, such as metallic ions, during synthetic processes may lead to mechanically stronger hydrogels . For instance, Hu et al have devised a hydrogel constructed through a two-step process of physical cross-linking, as illustrated in Figure A . Initially, xanthan gum (XG) and poly(vinyl alcohol) (PVA) undergo physical cross-linking via freeze–thaw cycles, utilizing hydrogen bonds and crystalline regions to establish the initial flexible network.…”

Section: Fabrication Strategiesmentioning

confidence: 99%

Challenges and Advances of Hydrogel-Based Wearable Electrochemical Biosensors for Real-Time Monitoring of Biofluids: From Lab to Market. A Review

Chenani,

Saeidi,

Rastkhiz

et al. 2024

Anal. Chem.

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“…By the freeze-thaw physical crosslinking method, Hu, et al (2023) developed a double-network hydrogel (DNCGel) consisting of PVA and xanthan gum (XG) chelated with ferric ion (Fe 3+ ). The DNGel3 hydrogels received the most attention in the study.…”

Section: Crosslinking By Freezing-thawingmentioning

confidence: 99%

Recent advances in the development of the physically crosslinked hydrogels and their biomedical applications

Siqueira,

França,

Souza

et al. 2023

RSD

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Hydrogels are three-dimensional networks formulated from natural or synthetic polymers with a high capacity to absorb and transport water in their structure. Hydrogels are prepared from the crosslinking of their polymeric chains, which involves two basic mechanisms: chemical crosslinking and physical crosslinking. In chemical crosslinking, hydrogels are held together by covalent bonds; while physically cross-linked hydrogels are produced by non-covalent interactions, such as hydrogen bonds, electrostatic interactions, and hydrophobic forces, among others. Physically cross-linked hydrogels are more similar to biological systems due to their assembly dynamics, so they have wide biomedical applications. The most used approaches in the preparation of hydrogels by physical crosslinking include freeze-thaw, formation of stereocomplexes, ionic interaction, hydrogen bonding, crystallization, and crosslinking by hydrophobic interactions. These approaches are briefly discussed in this review. Some biomedical applications of these hydrogels will also be discussed.

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Real-time monitoring flexible hydrogels based on dual physically cross-linked network for promoting wound healing

Cited by 28 publications

References 26 publications

Natural exosome-like nanoparticles derived from ancient medicinal insect Periplaneta americana L. as a novel diabetic wound healing accelerator

Natural exosome-like nanoparticles derived from ancient medicinal insect Periplaneta americana L. as a novel diabetic wound healing accelerator

Challenges and Advances of Hydrogel-Based Wearable Electrochemical Biosensors for Real-Time Monitoring of Biofluids: From Lab to Market. A Review

Recent advances in the development of the physically crosslinked hydrogels and their biomedical applications

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