Real-time monitoring of bead-based DNA hybridization in a microfluidic system: study of amplicon hybridization behavior on solid supports

Abstract

DNA hybridization phenomena occurring on solid supports are not understood as clearly as aqueous phase hybridizations and mathematical models cannot predict some empirically obtained results. Ongoing research has identified important...

Centrifugal microfluidic system for colorimetric sample-to-answer detection of viral pathogens

“…59 Furthermore, kinetic effects along with re-association of complementary strands can destabilize the probe/target duplex when amplicons with relatively long overhanging ends are used. 60,61…”