Real-time monitoring of copper ions-induced cytotoxicity by EIS cell chips

Primiceri, Elisabetta; Chiriaco, Maria; D’Amone, Eliana; Urso, Emanuela; Ionescu, Rodica Elena; Rizzello, Antonia; Maffia, Michele; Cingolani, R.; Rinaldi, R.; Maruccio, Giuseppe

doi:10.1016/j.bios.2010.04.032

Cited by 29 publications

(22 citation statements)

References 29 publications

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“…It is one of the most widely and early used method. It is applied in the biological and environmental analysis, such as protein (Zang et al 2015), cell (Primiceri et al 2010;, biological molecule (Beitollahi et al 2008;Mazloum-Ardakani et al 2011;Mazloum-Ardakani et al 2012), metal ion (Pelossof et al 2012;Sener et al 2014). PEC assay is a new analytical method for the faster, cheaper and simpler nucleic acid assay.…”

Section: Introductionmentioning

confidence: 99%

A visible light induced photoelectrochemical aptsensor constructed by aligned ZnO@CdTe core shell nanocable arrays/carboxylated g-C3N4 for the detection of Proprotein convertase subtilisin/kexin type 6 gene

Pang

Pan²,

Gao

et al. 2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

A visible light induced photoelectrochemical aptsensor constructed by aligned ZnO@CdTe core shell nanocable arrays/carboxylated g-C3N4 for the detection of Proprotein convertase subtilisin/kexin type 6 gene

Pang

Pan²,

Gao

et al. 2015

Biosensors and Bioelectronics

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“…A number of devices for the cell cultivation, single-cell, migration analysis, and cell based toxicity tests are reported. [1][2][3] In the microsystems, cells can be easily manipulated, and cellular environment can be precisely controlled. 4 Besides, there are also commercial microfluidic systems dedicated for cell a) Author to whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

et al. 2016

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The application of nanotechnology is important to improve research and development of alternative anticancer therapies. In order to accelerate research related to cancer diagnosis and to improve the effectiveness of cancer treatment, various nanomaterials are being tested. The main objective of this work was basic research focused on examination of the mechanism and effectiveness of the introduction of nanoencapsulated photosensitizers to human carcinoma (A549) and normal cells . Newly encapsulated hydrophobic indocyanine-type photosensitizer (i.e., IR-780) was subjected to in vitro studies to determine its release characteristics on a molecular level. The photosensitizers were delivered to carcinoma and normal cells cultured under model conditions using multiwell plates and with the use of the specially designed hybrid (poly(dimethylsiloxane) (PDMS)/ glass) microfluidic system. The specific geometry of our microsystem allows for the examination of intercellular interactions between cells cultured in the microchambers connected with microchannels of precisely defined length. Our microsystem allows investigating various therapeutic procedures (e.g., photodynamic therapy) on monoculture, coculture, and mixed culture, simultaneously, which is very difficult to perform using standard multiwell plates. In addition, we tested the cellular internalization of nanoparticles (differing in size, surface properties) in carcinoma and normal lung cells. We proved that cellular uptake of nanocapsules loaded with cyanine IR-780 in carcinoma cells was more significant than in normal cells. We demonstrated non cytotoxic effect of newly synthesized nanocapsules built with polyelectrolytes (PEs) of opposite surface charges: polyanion-polysodium-4-styrenesulphonate and polycation-poly(diallyldimethyl-ammonium) chloride loaded with cyanine IR-780 on human lung carcinoma and normal cell lines. However, the differences observed in the photocytotoxic effect between two types of tested nanocapsules can result from the type of last PE layer and their different surface charge. V C 2016 AIP Publishing LLC. [http://dx

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“…The whole device was assembled by a step of oxygen plasma exposure of PDMS (20 min at 0.5 mbar) and a rapid cleaning of the glass with the electrodes in piranha solution. These kinds of microfluidic chips have been already demonstrated to be able to detect cellular behavior in response to a chemical stimulus, 12,14 cell migration 13 and biorecognition events between immobilized antibodies and related antigens in aqueous solution. 11 Here a biochip to detect directly antibodies from serum samples for early diagnosis of PDAC is demonstrated.…”

Section: Biochip Structure and Fabricationmentioning

confidence: 99%

Towards pancreatic cancer diagnosis using EIS biochips

et al. 2013

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Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal cancers in Europe and the United States. It has a very low 5 years-survival rate and its diagnosis is often late and imprecise due to the lack of specificity of currently used markers for PDAC. As previously demonstrated PDAC patients' sera may contain autoantibodies towards phosphorylated α-enolase (ENOA), which in combination with other standard markers can increase specificity in diagnosis of PDAC. In this context we realized a microfluidic platform with integrated EIS biosensors. We achieved a specific antibodies detection by immobilizing onto electrodes peptides corresponding to a portion of ENOA. Phosphorylation of peptides was found to influence the recognition of antibodies in PDAC patients' sera detected by the developed biochip thus validating the EIS technique as a strong tool for quick, cost-saving and label-free analysis of serum samples. Biochip results are in agreement with those from traditional techniques, such as ELISA and western blot, but measurements are much more sensitive and specific, increasing the possibility of PDAC diagnosis. In addition this approach is faster and more reproducible compared to traditional techniques making the developed biochips ideal for a quick, cost-saving and label-free analysis of serum samples.

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Real-time monitoring of copper ions-induced cytotoxicity by EIS cell chips

Cited by 29 publications

References 29 publications

A visible light induced photoelectrochemical aptsensor constructed by aligned ZnO@CdTe core shell nanocable arrays/carboxylated g-C3N4 for the detection of Proprotein convertase subtilisin/kexin type 6 gene

A visible light induced photoelectrochemical aptsensor constructed by aligned ZnO@CdTe core shell nanocable arrays/carboxylated g-C3N4 for the detection of Proprotein convertase subtilisin/kexin type 6 gene

Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

Towards pancreatic cancer diagnosis using EIS biochips

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