Real Time Monitoring of the Interactions Between Pseudomonas Syringae Pv. Actinidiae and Actinidia Species

Spinelli, Francesco; Donati, Irene; Vanneste, J. L.; Costa, M.; Costa, Guglielmo

doi:10.17660/actahortic.2011.913.61

Cited by 50 publications

(62 citation statements)

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“…P. syringae pv. actinidiae, like all Pseudomonas syringae pathovars, has an epiphytic life on leaves (46,47) and is also systemic through the xylem vessels (48). The main pathway of dissemination of P. syringae pv.…”

Section: Discussionmentioning

confidence: 99%

Origin of the Outbreak in France of Pseudomonas syringae pv. actinidiae Biovar 3, the Causal Agent of Bacterial Canker of Kiwifruit, Revealed by a Multilocus Variable-Number Tandem-Repeat Analysis

Cunty

Cesbron

Poliakoff

et al. 2015

Appl Environ Microbiol

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The first outbreaks of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae biovar 3 were detected in France in 2010. P. syringae pv. actinidiae causes leaf spots, dieback, and canker that sometimes lead to the death of the vine. P. syringae pv. actinidifoliorum, which is pathogenic on kiwi as well, causes only leaf spots. In order to conduct an epidemiological study to track the spread of the epidemics of these two pathogens in France, we developed a multilocus variable-number tandemrepeat (VNTR) analysis (MLVA). MLVA was conducted on 340 strains of P. syringae pv. actinidiae biovar 3 isolated in Chile, China, France, Italy, and New Zealand and on 39 strains of P. syringae pv. actinidifoliorum isolated in Australia, France, and New Zealand. Eleven polymorphic VNTR loci were identified in the genomes of P. syringae pv. actinidiae biovar 3 ICMP 18744 and of P. syringae pv. actinidifoliorum ICMP 18807. MLVA enabled the structuring of P. syringae pv. actinidiae biovar 3 and P. syringae pv. actinidifoliorum strains in 55 and 16 haplotypes, respectively. MLVA and discriminant analysis of principal components revealed that strains isolated in Chile, China, and New Zealand are genetically distinct from P. syringae pv. actinidiae strains isolated in France and in Italy, which appear to be closely related at the genetic level. In contrast, no structuring was observed for P. syringae pv. actinidifoliorum. We developed an MLVA scheme to explore the diversity within P. syringae pv. actinidiae biovar 3 and to trace the dispersal routes of epidemic P. syringae pv. actinidiae biovar 3 in Europe. We suggest using this MLVA scheme to trace the dispersal routes of P. syringae pv. actinidiae at a global level.A gricultural systems are continuously afflicted by emerging infectious diseases (1), which can have significant agronomic and economic consequences. A thorough knowledge of the causal agent (propagation and contamination pathways, suitable environmental conditions, host range, and pathogenicity) is essential for determining and implementing efficient disease-management measures. Pathogen genotyping yields precious information for understanding the diversity and population structure of the bacterial organisms responsible for outbreaks. It enables hypotheses about the dispersion routes of bacterial populations or clonal lineages involved in epidemics. Multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) (2) is a powerful and portable genotyping method. It has been demonstrated that MLVA has a higher sensitivity and resolution than any other genotyping methods, such as pulsed-field gel electrophoresis (PFGE) and multilocus sequence type (MLST), applied for an in-depth study of bacteria populations or epidemic outbreaks (3, 4). The aim of MLVA is to use PCR to target the tandem repeats with a motif of more than five nucleotides and to analyze the variability of their pattern in order to discriminate isolates. Generally, VNTR loci evolve according to the stepwise mutation model (SMM) by gain or l...

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Section: Discussionmentioning

confidence: 99%

Origin of the Outbreak in France of Pseudomonas syringae pv. actinidiae Biovar 3, the Causal Agent of Bacterial Canker of Kiwifruit, Revealed by a Multilocus Variable-Number Tandem-Repeat Analysis

Cunty

Cesbron

Poliakoff

et al. 2015

Appl Environ Microbiol

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“…This approach demonstrated that Psa can infect the plant from stomata, flowers, leaf and fruit abscission scars, broken trichomes and natural wounds [44,45].…”

Section: The Infection Process and Disease Transmissionmentioning

confidence: 99%

“…The systemic displacement of the bacterium within the young twig most probably occurs via the xylem vessels [44], and this represents an uncommon route of plant colonization for a P. syringae pathovar.…”

Section: Disease Cyclementioning

confidence: 99%

“…In the summer, with temperatures increasing above 25 • C, the degree of infection is drastically reduced, even though the bacterium can colonize the host through the stomata and hydathodes [36,44].…”

Section: Disease Cyclementioning

confidence: 99%

“…The pathogen infects the host through stomata (Fig. 2), hydathodes, lenticels, trychomes, leaf scars or wounds, and can progress to the roots where it overwinters [7,44].…”

Section: Disease Cyclementioning

confidence: 99%

See 2 more Smart Citations

New insights on the bacterial canker of kiwifruit (Pseudomonas syringae pv. actinidiae)

Donati

Buriani

Cellini

et al. 2014

JBR

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Abstract. Since 2008, Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit has become the main pathogen of yellow and green fleshed kiwifruit. All major kiwifruit producing countries in the world have been affected by this bacterial pathogen, leading to substantial economic losses. This review presents the current knowledge on various aspects about the origin, epidemiology, detection and control strategies of Pseudomonas syringae pv. actinidiae.

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N-Acyl Homoserine Lactones and Lux Solos Regulate Social Behaviour and Virulence of Pseudomonas syringae pv. actinidiae

et al. 2019

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Real Time Monitoring of the Interactions Between Pseudomonas Syringae Pv. Actinidiae and Actinidia Species

Cited by 50 publications

References 0 publications

Origin of the Outbreak in France of Pseudomonas syringae pv. actinidiae Biovar 3, the Causal Agent of Bacterial Canker of Kiwifruit, Revealed by a Multilocus Variable-Number Tandem-Repeat Analysis

Origin of the Outbreak in France of Pseudomonas syringae pv. actinidiae Biovar 3, the Causal Agent of Bacterial Canker of Kiwifruit, Revealed by a Multilocus Variable-Number Tandem-Repeat Analysis

New insights on the bacterial canker of kiwifruit (Pseudomonas syringae pv. actinidiae)

N-Acyl Homoserine Lactones and Lux Solos Regulate Social Behaviour and Virulence of Pseudomonas syringae pv. actinidiae

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