Real-time particle tracking at 10,000 fps using optical fiber illumination

Otto, Oliver; Czerwinski, Fabian; Gornall, J. L.; Stober, Gunter; Oddershede, Lene B.; Seidel, Ralf; Keyser, Ulrich F.

doi:10.1364/oe.18.022722

Cited by 74 publications

(59 citation statements)

References 28 publications

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“…Despite this, we still find an error of less than 10 nm in each dimension down to an exposure time of 663 µs, corresponding to a frame rate of 1508 Hz. This can be further improved by increasing the illumination intensity [21]. Figures 3(e) and 3(f) show a small difference in the minimum error for each dimension.…”

Section: Resultsmentioning

confidence: 90%

Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Hay¹,

Gibson²,

Lee³

et al. 2014

Opt. Express

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Abstract:High-speed video stereo-microscopy relies on illumination from two distinct angles to create two views of a sample from different directions. The 3D trajectory of a microscopic object can then be reconstructed using parallax to combine 2D measurements of its position in each image. In this work, we evaluate the accuracy of 3D particle tracking using this technique, by extending the number of views from two to four directions. This allows us to record two independent sets of measurements of the 3D coordinates of tracked objects, and comparison of these enables measurement and minimisation of the tracking error in all dimensions. We demonstrate the method by tracking the motion of an optically trapped micro-sphere of 5 µm in diameter, and find an accuracy of 2-5 nm laterally, and 5-10 nm axially, representing a relative error of less than 2.5% of its range of motion in each dimension. Simpson, "An optically actuated surface scanning probe," Opt. Express 20, 29679-29693 (2012). 12. C. Alpmann, R. Bowman, M. Woerdemann, M. Padgett, and C. Denz, "Mathieu beams as versatile light moulds for 3d micro particle assemblies," Opt. Express 18, 26084-26091 (2010

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Section: Resultsmentioning

confidence: 90%

Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Hay¹,

Gibson²,

Lee³

et al. 2014

Opt. Express

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“…This is important when manipulating particles over the comparatively large distances, and hence high speeds, accessible using a low-magnification objective. Our implementation uses a high-speed camera [26] and a fast SLM with optimised hologram generation to increase the bandwidth of our system by an order of magnitude compared to the previous work [18]. Also, the ability to axially reposition the foci increases the maximum force available to us compared to a fixed-focal-plane system.…”

Section: Discussionmentioning

confidence: 99%

Position clamping in a holographic counterpropagating optical trap

Bowman¹,

Jesacher²,

Thalhammer³

et al. 2011

Opt. Express

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Optical traps consisting of two counterpropagating, divergent beams of light allow relatively high forces to be exerted along the optical axis by turning off one beam, however the axial stiffness of the trap is generally low due to the lower numerical apertures typically used. Using a high speed spatial light modulator and CMOS camera, we demonstrate 3D servocontrol of a trapped particle, increasing the stiffness from 0.004 to 1.5 µNm −1 . This is achieved in the "macro-tweezers" geometry [Thalhammer, J. Opt. 13, 044024 (2011); Pitzek, Opt. Express 17, 19414 (2009)], which has a much larger field of view and working distance than single-beam tweezers due to its lower numerical aperture requirements. Using a 10×, 0.2 NA objective, active feedback produces a trap with similar effective stiffness to a conventional single-beam gradient trap, of order 1 µNm −1 in 3D. Our control loop has a round-trip latency of 10ms, leading to a resonance at 20Hz. This is sufficient bandwidth to reduce the position fluctuations of a 10 µm bead due to Brownian motion by two orders of magnitude. This approach can be trivially extended to multiple particles, and we show three simultaneously position-clamped beads.

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“…We verify the stability of the instrument by measuring the Allan variance, [22][23][24] demonstrating position measurement to an accuracy better than 1 nm for a measurement time between 0.5 and 50 s. The system is compatible with a wide range of microscopy techniques including brightfield, darkfield, 25 stereo, 26 and fluorescence as well as particle tracking using video microscopy. 23,27 II. SYSTEM CONFIGURATION A schematic of the optical system is shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

A compact holographic optical tweezers instrument

Gibson

Bowman

Linnenberger

et al. 2012

Review of Scientific Instruments

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Visual and dynamical measurement of Rayleigh-Benard convection by using fiber-based digital holographic interferometry J. Appl. Phys. 112, 113113 (2012) Guide-star-based computational adaptive optics for broadband interferometric tomography Appl. Phys. Lett. 101, 221117 (2012) Limits of elemental contrast by low energy electron point source holography J. Appl. Phys. 110, 094305 (2011) Cantilever biosensor reader using a common-path, holographic optical interferometer Appl. Phys. Lett. 97, 221110 (2010) Extended depth of focus in a particle field measurement using a single-shot digital hologram Appl. Phys. Lett. 95, 201103 (2009) Additional information on Rev. Sci. Instrum. Holographic optical tweezers have found many applications including the construction of complex micron-scale 3D structures and the control of tools and probes for position, force, and viscosity measurement. We have developed a compact, stable, holographic optical tweezers instrument which can be easily transported and is compatible with a wide range of microscopy techniques, making it a valuable tool for collaborative research. The instrument measures approximately 30×30×35 cm and is designed around a custom inverted microscope, incorporating a fibre laser operating at 1070 nm. We designed the control software to be easily accessible for the non-specialist, and have further improved its ease of use with a multi-touch iPad interface. A high-speed camera allows multiple trapped objects to be tracked simultaneously. We demonstrate that the compact instrument is stable to 0.5 nm for a 10 s measurement time by plotting the Allan variance of the measured position of a trapped 2 μm silica bead. We also present a range of objects that have been successfully manipulated.

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Real-time particle tracking at 10,000 fps using optical fiber illumination

Cited by 74 publications

References 28 publications

Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Four-directional stereo-microscopy for 3D particle tracking with real-time error evaluation

Position clamping in a holographic counterpropagating optical trap

A compact holographic optical tweezers instrument

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