Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of
            <i>Legionella</i>
            spp. and
            <i>Legionella pneumophila</i>

Herpers, Bjorn L.; Jongh, B. M. De; Zwaluw, Kim van der; Hannen, Erik J. van

doi:10.1128/jcm.41.10.4815-4816.2003

Cited by 77 publications

(43 citation statements)

References 15 publications

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“…and provide rapid results. Most diagnostic PCR assays have specific target regions within the 16S rRNA genes (1,18,28,30,32,36,37), the 23S-5S spacer region (16), 5S rRNA gene (14), or the macrophage inhibitor potentiator gene (mip) (1,19,20,21,26,29,30,37,38). Thus far, results obtained from in vitro evaluations and small patient series have been encouraging.…”

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confidence: 95%

Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical Practice

et al. 2008

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Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical Practice

et al. 2008

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“…Blood samples obtained on the first day of fever were stored at -80˚C until the serum PCT and CRP levels were measured. The serum CRP level was determined using the (8). Urine was sampled for antigen testing on Streptococcus pneumoniae and Legionella pneumophila (Binax NOW; Binax, Scarborough, ME, USA) (9,10).…”

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confidence: 99%

Infectious background of febrile advanced lung cancer patients who received chemotherapy

et al. 2010

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Abstract. The study aimed to determine the diagnostic utility of procalcitonin (PCT) in order to discriminate between infective fever and fever due to inflammation in febrile advanced lung cancer patients treated with cytotoxic chemotherapy. A total of 121 patients with advanced lung cancer, treated with a cytotoxic chemotherapy regimen between September 2007 and September 2008 at Kyoto University Hospital, were recruited. Blood samples were obtained on the first day of the fever. Serum c-reactive protein (CRP) and PCT levels were measured. At least two blood cultures were performed, and sputum was taken for Gram staining and culture. There were 71 episodes in 61 patients in the 12 months of the study, representing 50.4% of our study population. A total of 41 patients (57.7%) were diagnosed with pneumonia using imaging modalities, 6 (8.5%) with bacteremia using blood culture and 4 (5.6%) with urinary tract infections using urine culture. Among the 41 pneumonia cases, culture from sputum revealed pathologic bacteria in 21 (51.2%) and fungal disease in 14 (34.1%) cases. Among the 71 febrile episodes, serum procalcitonin and CRP were measured in 50 episodes. Serum procalcitonin-positive patients showed poor outcomes on antibiotics therapy (Fisher's exact test, p=0.042). Furthermore, serum procalcitonin positivity was able to discriminate infective fever from fever due to inflammation (Chi-square test, p=0.001). We showed the causative organisms of febrile advanced lung cancer patients who received cytotoxic chemotherapy, as well as the possibility of PCT to discriminate infective fever from fever due to inflammation.

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“…The mip PCRs were designed to specifically detect L. pneumophila, while the 16S rRNA gene PCRs were designed to detect other Legionella species as well (3,(8)(9)(10)(11)14). This is clearly reflected in the scores for sample 10, which was spiked with L. longbeachae.…”

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confidence: 99%

“…A range of molecular methods is described in the literature for the detection of Legionella spp. (1,(3)(4)(5)(6)(7)(8)(10)(11)(12)(13)(14). In this report we compared the methods that are performed by nine laboratories in The Netherlands.…”

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Multicenter Comparison of Molecular Methods for Detection of Legionella spp. in Sputum Samples

et al. 2007

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Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis.

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Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila

Cited by 77 publications

References 15 publications

Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical Practice

Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical Practice

Infectious background of febrile advanced lung cancer patients who received chemotherapy

Multicenter Comparison of Molecular Methods for Detection of Legionella spp. in Sputum Samples

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