Real-time polymerase chain reaction and intraocular antibody production for the diagnosis of viral versus toxoplasmic infectious posterior uveitis

Errera, Marie‐Hélène; Goldschmidt, Pablo; Batellier, L.; Degorge, Sandrine; Héron, Emmanuel; Laroche, L.; Sahel, José‐Alain; Westcott, Mark; Chaumeil, C.

doi:10.1007/s00417-011-1724-7

Cited by 48 publications

(31 citation statements)

References 36 publications

(53 reference statements)

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“…Errera et al recently reported the sensitivity and specificity of real-time PCR and GWC in intraocular samples of posterior uveitis patients' collected at different time points during the clinical course. GWC showed higher diagnostic values for T. gondii detection, especially when the test was carried out late in the disease course [37]. However, in our study, GWC for T. gondii was found positive in one patient only.…”

Section: Discussioncontrasting

confidence: 77%

“…In our study the aqueous humor and the vitreous humor samples had similar diagnostic values. It is reported that the diagnostic efficacy of aqueous humor analysis can be improved with the concurrent use of GWC and PCR [7,37]. Errera et al recently reported the sensitivity and specificity of real-time PCR and GWC in intraocular samples of posterior uveitis patients' collected at different time points during the clinical course.…”

Section: Discussionmentioning

confidence: 99%

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Infectious causes of posterior uveitis and panuveitis in Thailand

et al. 2012

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Section: Discussioncontrasting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Infectious causes of posterior uveitis and panuveitis in Thailand

et al. 2012

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“…The diagnosis of ocular toxoplasmosis was always confirmed by intraocular fluid assessment. [8][9][10][11] Diagnosis of presumed ocular toxoplasmosis was based on typical clinical features of unilateral focal necrotizing retinitis sometimes associated with typical old pigmented scars. All other specific diagnoses were performed according to current diagnostic criteria.…”

Section: Assessment Of Determinants and Outcomesmentioning

confidence: 99%

Visual outcomes and ocular morbidity of patients with uveitis referred to a tertiary center during first year of follow-up

Groen

Ramdas

Hoog

et al. 2016

Eye

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Purpose To describe the visual outcomes and morbidity of newly referred uveitis patients. Methods Retrospective cohort study of 133 newly referred uveitis patients with active uveitis who required care in a tertiary center for at least 1 year. Main outcomes were best-corrected visual acuity (BCVA) at referral and 1 year after referral, duration of visual impairment, systemic medications used, as well as all complications and surgeries during the first year of follow-up. Generalized estimating equation models was used to assess prognosticators for poor BCVA. Results The mean age at onset of uveitis was 43 years. The proportion of patients with at least one eye with BCVA ≤ 0.3 decreased from 35% at referral to 26% (P = 0.45) at 1-year follow-up. The mean duration of visual impairment in the first year after referral was 4 months per affected eye. At 1-year follow-up, bilateral visual impairment was observed in 4% but at least one ocular complication developed in 66% and 30% of patients required at least one intraocular surgery. Systemic immunosuppressive treatment was required in 35% of patients and the mean number of visits to ophthalmologist was 11 per year, while 8% of patients required hospital admission. Prognosticators for poor visual outcome included surgery undergone before referral (odds ratio (OR), 3; 95% CI, 1-11; P = 0.047), visual impairment at referral (OR, 21; 95% CI, Po0.001), and glaucoma before referral (OR, 7; 95% CI, 2-28; P = 0.007). Conclusions Patients with severe uveitis had a favorable BCVA 1 year after referral with only 4% of patients having bilateral visual impairment. This, in contrast to the prolonged duration of visual impairment during the first year of follow-up and the demanding care.

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“…It's very important to remember that PCR is strongly positive in the first days after ocular lesion clinical manifestations, whereas immunoblotting sensitivity that is low at the beginning increases along time, so time interval between ocular manifestations beginning and humour aqueous analysis influence strongly intraocular antibody synthesis detection (4).…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii and Epstein Barr retinochoroiditis in an immunocompromised patient

et al. 2016

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We report the case of a patient aged 52 years old, who has been hospitalised at Ophthalmic Hospital in Turin (Italy) with entrance diagnosis of right eye anterior and posterior uveitis and retinopathy of suspect micotic origin. Patient underwent vitreal biopsy. Laboratory evaluation with polymerase chain reaction (PCR) method performed on vitreous sample showed the presence of Epstein Barr virus (EBV) and Toxoplasma gondii (TG). PCR should be performed either in eyes samples or in blood samples, furthermore, it is essential to include EBV and TG in the differential diagnosis of ocular lesions.

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Real-time polymerase chain reaction and intraocular antibody production for the diagnosis of viral versus toxoplasmic infectious posterior uveitis

Cited by 48 publications

References 36 publications

Infectious causes of posterior uveitis and panuveitis in Thailand

Infectious causes of posterior uveitis and panuveitis in Thailand

Visual outcomes and ocular morbidity of patients with uveitis referred to a tertiary center during first year of follow-up

Toxoplasma gondii and Epstein Barr retinochoroiditis in an immunocompromised patient

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