Real-Time Polymerase Chain Reaction Versus Conventional PCR: A Comparison between two Methods for the Detection of<i>Fusobacterium Nucleatum</i>in Saliva, Nasopharyngeal Secretion and Middle Ear Effusion Samples

Topçuoğlu, Nursen; Paltura, Ceki; Külekçi, Mehmet; Ustek, Duran; Külekçi, Güven

doi:10.5504/bbeq.2013.0022

Cited by 10 publications

(5 citation statements)

References 20 publications

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“…Thus, quantitative real-time PCR, which allows monitoring of the exponential phase, has been added to the potential methods for examining the composition of oral biofilm samples [17,18]. It is an excellent method providing quantification of the level of selected species in biofilm samples, has the advantage of providing rapid, accurate, sensitive and quantitative detection [19].…”

Section: Discussionmentioning

confidence: 99%

16S rRNA based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis

Topçuoğlu

Külekçi

2015

Anaerobe

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Section: Discussionmentioning

confidence: 99%

16S rRNA based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis

Topçuoğlu

Külekçi

2015

Anaerobe

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“…In clinical research, there are already some comparisons between PCR and quantitative PCR 2 , 4 , 6 , 11 , 25 , 36 (qPCR); these studies indicated a higher sensitivity of qPCR compared to PCR 3 . The mechanisms by which the qPCR can be more sensitive than PCR have been described 14 , 35 , among them, qPCR perform the quantitation of the target gene during exponential amplification avoiding problems that are associated with the so-called 'end-point' of PCR in which amplicons are only analyzed after completion of the final PCR cycle 35 ; it is only during this exponential phase of the PCR that it will be possible to determine the starting amount of template 14 .…”

Section: Introductionmentioning

confidence: 99%

Quantitative vs. Conventional PCR for Detection of Human Adenoviruses in Water and Sediment Samples

Staggemeier

Bortoluzzi

Heck

et al. 2015

Rev. Inst. Med. trop. S. Paulo

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SUMMARYHuman Adenoviruses (HAdV) are notably resistant in the environment. These agents may serve as effective indicators of fecal contamination, and may act as causative agents of a number of different diseases in human beings. Conventional polymerase chain reaction (PCR) and, more recently, quantitative PCR (qPCR) are widely used for detection of viral agents in environmental matrices. In the present study PCR and SYBR(r)Green qPCR assays were compared for detection of HAdV in water (55) and sediments (20) samples of spring and artesian wells, ponds and streams, collected from dairy farms. By the quantitative methodology HAdV were detected in 87.3% of the water samples and 80% of the sediments, while by the conventional PCR 47.3% and 35% were detected in water samples and sediments, respectively.

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“…[ 38 48 54 55 56 ] Previous studies suggest that real-time PCR is more efficient than microbiological culture and conventional PCR. [ 57 58 ] Despite its simplicity and accuracy, PCR-based diagnostic procedures are still not extensively used in private clinics. Owing to the need for a thermal cycler device, the use of PCR-based diagnostic techniques as a routine diagnostic tool is a challenging task.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis

Sangolli,

Kugaji,

Ray

et al. 2024

Journal of Indian Society of Periodontology

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Background: Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by oral pathogens. Porphyromonas gingivalis is a Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and is regarded as a primary etiological factor in the progression of periodontitis. Rapid, highly senstitive and specific detection methods are emerging. The present study aimed to evaluate the loop-mediated isothermal amplification (LAMP) technique for efficiently detecting P. gingivalis from subgingival plaque samples of chronic periodontitis patients. Materials and Methods: This study included 50 subgingival plaque samples from patients suffering from chronic periodontitis. The DNA (Deoxyribonucleic acid) was extracted by the “modified proteinase K” method. A set of six primers, targeting the pepO gene of P. gingivalis, was used for conducting LAMP. The amplification was visualized by naked-eye detection and agarose electrophoresis. Conventional polymerase chain reaction (PCR) and real-time qantitative PCR (qPCR) were carried out by targeting the 16SrRNA (16S ribosomal ribonucleic acid) gene of P. gingivalis. Results: The results showed that LAMP detected P. gingivalis in 40 out of 50 samples (80%). Whereas, qPCR and conventional PCR technique detected P. gingivalis in 38 (76%) and 33 (66%) samples respectively. The sensitivity and specificity of the LAMP method were 94.87% and 90.90%, respectively. With qPCR, the sensitivity and specificity were found to be 92.30% and 81.81%, respectively, whereas, with conventional PCR, it was found to be 76.92% and 72.72%, respectively. Conclusion: LAMP is an efficient technique for quick, accurate, and reliable identification of P. gingivalis from subgingival plaque samples. The technique needs to be validated analytically, and further studies can be conducted by taking saliva and/or gingival crevicular fluid samples from periodontitis patients.

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Real-Time Polymerase Chain Reaction Versus Conventional PCR: A Comparison between two Methods for the Detection ofFusobacterium Nucleatumin Saliva, Nasopharyngeal Secretion and Middle Ear Effusion Samples

Cited by 10 publications

References 20 publications

16S rRNA based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis

16S rRNA based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis

Quantitative vs. Conventional PCR for Detection of Human Adenoviruses in Water and Sediment Samples

Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis

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