16S rRNA based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis

Topçuoğlu, Nursen; Külekçi, Güven

doi:10.1016/j.anaerobe.2015.01.011

Cited by 30 publications

(32 citation statements)

References 32 publications

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“…These results corroborate with several studies reporting high levels and incidence of these pathogenic species in generalized chronic periodontitis and peri‐implantitis (Holt & Ebersole ; Mysak et al. ; Topcuoglu & Kulekci ). Yet, there is no consensus on the literature regarding the effective quantity of determined species needed to originate an inflammatory process.…”

Section: Discussionsupporting

confidence: 91%

“…Bacterial species considered as the main agents of periodontitis, P. gingivalis, T. forsythia, and T. denticola, referred to as the red complex bacteria, and other species strongly associated with periodontal disease, P. intermedia and A. actinomycetemcomitans, were detected in moderate to elevated counts in both teeth (baseline) and implant-related sites (after 4 and 8 months of function). These results corroborate with several studies reporting high levels and incidence of these pathogenic species in generalized chronic periodontitis and peri-implantitis (Holt & Ebersole 2005;Mysak et al 2014;Topcuoglu & Kulekci 2015). Yet, there is no consensus on the literature regarding the effective quantity of determined species needed to originate an inflammatory process.…”

Section: Discussionsupporting

confidence: 87%

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Microbiological and clinical outcomes of fixed complete‐arch mandibular prostheses supported by immediate implants in individuals with history of chronic periodontitis

Gomes

Sartori

Able

et al. 2016

Clinical Oral Implants Res

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Total and individual counts of target species did not differ between teeth and implants for 8 months of investigation. The mean proportions of pathogenic and non-pathogenic species remained unaltered, and no clinical complications were reported over time. Data obtained suggest that immediate loading of complete mandibular prostheses retained by implants placed immediately after extraction may be a viable treatment option for edentulous individuals with previous history of periodontal disease.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Microbiological and clinical outcomes of fixed complete‐arch mandibular prostheses supported by immediate implants in individuals with history of chronic periodontitis

Gomes

Sartori

Able

et al. 2016

Clinical Oral Implants Res

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show abstract

“…The following microorganisms have been previously shown to be associated with periodontal disease: Abiotrophia elegans (64,65), Bacteroides-like (66), Dialister (67,68,69), Filifactor alocis (70,71,72,73,74), Fusobacterium nucleatum (15,42,75,76,77,78), Granulicatella elegans (79), Haemophilus (70,80,81), Haemophilus segnis (82,83) Megasphaera (4,84), Mogibacterium (85,86), Staphylococcus epidermidis (87), Tannerella forsythia (71,77,89,90). These findings support our findings that these microorganisms are signatures of periodontitis in the human oral microbiome.…”

Section: Discussionmentioning

confidence: 99%

“…Oligonucleotide microarray technology has been used to profile microbial communities for quite some time (13,14,15,16,17). A new version of the methodology, the Gene Meter, was recently introduced (although not explicitly named “Gene Meter” in refs.…”

Section: Introductionmentioning

confidence: 99%

Microbial Signatures of Oral Dysbiosis, Periodontitis and Edentulism Revealed by Gene Meter Methodology

Hunter

Pozhitkov

Noble

2016

Preprint

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Conceptual models suggest certain microorganisms (e.g., the red complex) are indicative of a specific disease state (e.g., periodontitis); however, recent studies have questioned the validity of these models. Here, the abundances of 500+ microbial species were determined in 16 patients with clinical signs of one of the following oral conditions: periodontitis, established caries, edentulism, and oral health. Our goal was to determine if the abundances of certain microorganisms reflect dysbiosis or a specific clinical condition that could be used as a signature for dental research. Microbial abundances were determined by the analysis of 138,718 calibrated probes using Gene Meter methodology. Each 16S rRNA gene was targeted by an average of 194 unique probes (n=25 nt). The calibration involved diluting pooled gene target samples, hybridizing each dilution to a DNA microarray, and fitting the probe intensities to adsorption models. The fit of the model to the experimental data was used to assess individual and aggregate probe behavior; good fits (R2>0.90) were retained for back-calculating microbial abundances from patient samples. The abundance of a gene was determined from the median of all calibrated individual probes or from the calibrated abundance of all aggregated probes. With the exception of genes with low abundances (< 2 arbitrary units), the abundances determined by the different calibrations were highly correlated (r ∼1.0). Seventeen genera were classified as signatures of dysbiosis because they had significantly higher abundances in patients with periodontitis and edentulism when contrasted with health. Similarly, 13 genera were classified as signatures of periodontitis, and 14 genera were classified as signatures of edentulism. The signatures could be used, individually or in combination, to assess the clinical status of a patient (e.g., evaluating treatments such as antibiotic therapies). Comparisons of the same patient samples revealed high false negatives (45%) for next-generation-sequencing results and low false positives (7%) for Gene Meter results.

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“…Porphyromonas Bacteroides In clinical practice, microarrays can be used to detect and quantify the specific pathogens responsible for periodontitis [37]. They can also be utilized to identify whether the pathogens are the ones more likely to be associated with refractory periodontitis, and to assess the efficacy of periodontal therapy [38].…”

Section: Conventional Methods Molecular Methods Referencesmentioning

confidence: 99%

Oral Health: The Need for Both Conventional Microbial and Molecular Characterization

Friedman¹,

Alizadeh²,

Loewy³

2017

High-Throughput

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This study aims to consider the microbial distribution in oral disease, as well as gene analysis and expression, in elucidating: 1, the fundamental underpinnings of oral disease, and 2, the potential relationship between oral diseases and systemic health. A key focus is identifying the microbiota associated with oral disease manifestations characterized by both conventional microbiological and molecular methods. Variations in the observed microbial populations characterized by conventional and molecular approaches have been identified for caries, periodontitis, peri-implantitis, and stomatitis. The discovery of therapeutic approaches for oral disease will require comprehensive microbial and genomic analysis. This study evaluated the current state of the relevant microbial and genomic information for several prevalent oral diseases.

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16S rRNA based microarray analysis of ten periodontal bacteria in patients with different forms of periodontitis

Cited by 30 publications

References 32 publications

Microbiological and clinical outcomes of fixed complete‐arch mandibular prostheses supported by immediate implants in individuals with history of chronic periodontitis

Microbiological and clinical outcomes of fixed complete‐arch mandibular prostheses supported by immediate implants in individuals with history of chronic periodontitis

Microbial Signatures of Oral Dysbiosis, Periodontitis and Edentulism Revealed by Gene Meter Methodology

Oral Health: The Need for Both Conventional Microbial and Molecular Characterization

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