The real-time and accuracy of motion classification plays an essential role for the elderly or frail people in daily activities. This study aims to determine the optimal feature extraction and classification method for the activities of daily living (ADL). In the experiment, we collected surface electromyography (sEMG) signals from thigh semitendinosus, lateral thigh muscle, and calf gastrocnemius of the lower limbs to classify horizontal walking, crossing obstacles, standing up, going down the stairs, and going up the stairs. Firstly, we analyzed 11 feature extraction methods, including time domain, frequency domain, time-frequency domain, and entropy. Additionally, a feature evaluation method was proposed, and the separability of 11 feature extraction algorithms was calculated. Then, combined with 11 feature algorithms, the classification accuracy and time of 55 classification methods were calculated. The results showed that the Gaussian Kernel Linear Discriminant Analysis (GK-LDA) with WAMP had the highest classification accuracy rate (96%), and the calculation time was below 80 ms. In this paper, the quantitative comparative analysis of feature extraction and classification methods was a benefit to the application for the wearable sEMG sensor system in ADL.