Realization of the Stereotaxic Surgery

Ferry, Barbara; Gervasoni, Damien; Vogt, Catherine

doi:10.1007/978-2-8178-0472-9_6

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…To facilitate visualization of the craniometric points bregma and lambda on the cranial surface and to enhance posterior cannula fixation, the periosteum was scraped using a microcurette (Model 10080-05; Fine Surgical Tools F.S.T., Chandler, AZ, USA) and dried with a cotton swab moistened with 70% ( v / v ) ethanol. The coordinates of the bregma and lambda landmarks were then measured to accurately position them in the dorso-ventral (DV), medio-lateral (ML) and antero-posterior (AP) planes [ 1 , 36 , 37 ] and readjusted whenever the difference between coordinates exceeded 0.05 mm. Once a “flat-skull” position was reached, a 0.5 mm diameter craniotomy was drilled in the skull at the specific coordinates AP= −0.70 mm and ML= −1.26 mm ( Figure 3 ).…”

Section: Methodsmentioning

confidence: 99%

Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents

Pérez-Martín¹,

Coto-Vilcapoma

Castilla-Silgado

et al. 2023

Animals

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Stereotaxic surgeries enable precise access to specific brain regions, being of particular interest for chronic intracerebroventricular drug delivery. However, the challenge of long-term studies at this level is to allow the implantation of drug storage devices and their correct intrathecal connection while guaranteeing animal welfare during the entire study period. In this study, we propose an optimized method for safe intrathecal device implantation, focusing on preoperative, intraoperative, and postoperative procedures, following the 3Rs principle and animal welfare regulations. Our optimized protocol introduces three main refinements. Firstly, we modify the dimensions of the implantable devices, notably diminishing the device-to-mouse weight ratio. Secondly, we use a combination of cyanoacrylate tissue adhesive and UV light-curing resin, which decreases surgery time, improves healing, and notably minimizes cannula detachment or adverse effects. Thirdly, we develop a customized welfare assessment scoresheet to accurately monitor animal well-being during long-term implantations. Taken together, these refinements positively impacted animal welfare by minimizing the negative effects on body weight, surgery-related complications, and anxiety-like behaviors. Overall, the proposed refinements have the potential to reduce animal use, enhance experimental data quality, and improve reproducibility. Additionally, these improvements can be extended to other neurosurgical techniques, thereby advancing neuroscience research, and benefiting the scientific community.

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Section: Methodsmentioning

confidence: 99%

Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents

Pérez-Martín¹,

Coto-Vilcapoma

Castilla-Silgado

et al. 2023

Animals

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“…Body temperature was maintained at 37°C with a thermostatically controlled blanket. The surgical procedure to introduce an indwelling cannula (0.6 mm diameter) into the lateral cerebral ventricle was followed under the recommendations of Ferry, Gervasoni, and Vogt (2014). Briefly, a sagittal incision of the scalp was made at the midline from the occipital protuberance to the eyes to expose the frontal and parietal bones.…”

Section: Experimental Designmentioning

confidence: 99%

Participation of TRPV1 in the activity of the GnRH system in male rats

Surkin

Dmytrenko

Giorgio

et al. 2020

Eur J of Neuroscience

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GnRH neuron activity is under the influence of multiple stimuli, including those coming from the endocannabinoid and the immune systems. Since it has been previously suggested that some of the main elements controlling the GnRH pulse generator possess the TRPV1 receptor, the aim of the present study was to evaluate the participation of the hypothalamic TRPV1, through its pharmacological blockade, in the activity of the hypothalamic‐pituitary‐testicular axis in male rats under basal or acute inflammatory conditions. Our hypothesis was based on the idea that the hypothalamic TRPV1 participates in the synthesis of the main neuromodulatory signals controlling GnRH, and therefore the reproductive axis. Our results showed that the hypothalamic TRPV1 blockade induced pro‐inflammatory effects by increasing Tnfα and Il‐1β mRNA hypothalamic levels and inhibited the reproductive axis by affecting Gnrh, Kiss1 and Rfrp3 mRNA levels and decreasing plasma levels of luteinizing hormone and testosterone under basal conditions, without significant additive effects in rats exposed to systemic LPS. Altogether, these results suggest that the hypothalamic TRPV1 receptor participates in the regulation of the GnRH system, probably by modulating immune‐dependent mechanisms.

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Realization of the Stereotaxic Surgery

Cited by 2 publications

References 34 publications

Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents

Refining Stereotaxic Neurosurgery Techniques and Welfare Assessment for Long-Term Intracerebroventricular Device Implantation in Rodents

Participation of TRPV1 in the activity of the GnRH system in male rats

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