Rearrangement of Actin Microfilaments in the Development of Olfactory Receptor Cells in Fish

Abstract

At present, it remains poorly understood how the olfactory neuron migrates through the thick neuroepithelium during its maturation from a stem cell and how it develops a specific sensitivity to environmental odorants after maturation. We investigated the cytochemical features associated with the development of olfactory cells before and after the incorporation of dendrites into the surface of the olfactory epithelium. Using cytochemical staining for the actin cytoskeleton and other cell components, we found that immature neurons acquire a streamlined shape that resembles a «hot-dog» during their migration: a dense layer of actin microfilaments forms beneath the surface membrane of the growing dendrite, and the bulk of the nuclear material moves inside this layer. We have found that when the cell makes contact with its environment, the dendritic terminal develops a wide actin layer, inside which a pore is formed. It is assumed that the functional receptors of odorants generate across this pore the first intracellular signal from environmental water-soluble odorants. These data illustrate the important role of the cytoskeleton in the differentiation of olfactory cells.

Long-range migration of centrioles to the apical surface of the olfactory epithelium

“…En face imaging of the apical surface allowed us to identify dendritic knobs ensheathed in sustentacular cells with borders rich in filamentous actin ( Figure 1B and C ; Liang, 2018 ). In side views of the olfactory epithelium, migrating centrioles were identified as elongated clusters of puncta, in accordance with previous reports ( Figure 1D ; Heist and Mulvaney, 1968 ; Klimenkov et al, 2018 ; Mulvaney and Heist, 1971 ; Ying et al, 2014 ).…”

Long-range migration of centrioles to the apical surface of the olfactory epithelium

“…En face imaging of the apical surface allowed us to identify dendritic knobs ensheathed in sustentacular cells with borders rich in filamentous actin (Fig 1B , 1C) (Liang, 2018). In side views of the olfactory epithelium, migrating centrioles were identified as elongated clusters of puncta, in accordance with previous reports (Fig 1D) (Heist & Mulvaney, 1968;Klimenkov et al, 2018;Mulvaney & Heist, 1971, Ying et al, 2014.…”

The effects of sound pollution as a stress factor for the Baikal coregonid fish

“…Histological processing of the samples with sensory epithelium was performed according to the standard technique (Klimenkov et al, 2018). The sensory epithelium was fixed for 30 min in a 2% paraformaldehyde (Sigma-Aldrich Co. LLC, USA, Cat.…”