Reasons for possible failure of inoculation to enhance biodegradation

Goldstein, Robert M.; Mallory, Larry M.; Alexander, Martin

doi:10.1128/aem.50.4.977-983.1985

Cited by 320 publications

(138 citation statements)

References 21 publications

Supporting

Mentioning

129

Contrasting

Order By: Relevance

“…As an alternative, bioaugmentation with carefully selected strains may improve the opportunity to create more reproducible systems that enhance degradative ability, and by pre-emptive colonization, reduce invasion by opportunistic populations with little degradative ability. Evidence suggests mixed inoculation would be better suited for MWF treatment, as bioaugmentation of multisubstrate habitats (such as wastewater, groundwater, soil or slurry) with pure cultures, has been typically proven ineffective (Goldstein et al ., 1985;Bouchez et al ., 2000). Introduction of acclimatised microbial populations, isolated from a contaminated habitat or target waste, may be a better option (Beaulieu et al ., 2000;Vogel and Walter, 2001), as these populations have been shown to be more resistant to extreme ambient conditions and even predation (Fewson, 1988;Otte et al ., 1994;Hamer, 1997).…”

Section: Introductionmentioning

confidence: 99%

Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal‐working fluid

Gast

Whiteley

Thompson

2004

Environmental Microbiology

View full text Add to dashboard Cite

In order for established bioreactors to be effective for treating chemically mixed wastes such as metal working fluids (MWF) it is essential that they harbour microbial populations that can maintain sufficient active biomass and degrade each of the chemical constituents present. In this study we investigated the effectiveness of a bacterial consortium composed of four species (Clavibacter michiganensis, Methylobacterium mesophilicum, Rhodococcus erythropolis and Pseudomonas putida), assembled on the basis of their apparent ubiquity in waste MWF, degradation ability and tolerance to fluctuating chemistry of the waste. The temporal dynamics of the inoculum and its effects on the fate of individual chemical components of the waste were studied, by regular sampling, over 400 h. Using a complementary approach of culture with chemotaxonomic (FAME) analysis and applying group specific probes (FISH), the inoculum was found to represent a significant component of the community in bioreactors with and without presence of indigenous MWF populations. In addition, the reduction in the COD by the consortium was approximately 85% of the total pollution load, and 30-40% more effectively than any other treatment (indigenous MWF community alone or activated sludge). Furthermore, all the chemical constituents, including the biocide (a formaldehyde release agent) demonstrated > 60% reduction. Many chemical components of the MWF proved to be recalcitrant in the other treatments. The results of this study confirm that assemblage of an inoculum, based on a comprehensive knowledge of the indigenous microbial community, in the target habitat, is a highly effective way of selecting microbial populations for bioaugmentation of bioreactors.

show abstract

Section: Introductionmentioning

confidence: 99%

Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal‐working fluid

Gast

Whiteley

Thompson

2004

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Although some full-scale experiments with bioaugmentation have been reported as being successful (Stevens, 1989;Stephenson and Stephenson, 1992;Rittmann and Whiteman, 1994), other studies conducted by independent laboratories have often observed that bioaugmentation had little effect (for a review, see Stephenson and Stephenson, 1992). Problems concerning the adaptation of the inoculated microorganisms, the insuf®ciency of substrate, competition between the introduced species and the indigenous biomass, and grazing by protozoa have been given as possible reasons for the failure of the experiments (Goldstein et al, 1985). However, the interpretation of the results of these studies often suffered from a lack of ecological data about the fate and activity of the inoculated organisms and about the reaction of the indigenous microbial communities (Rittmann and Whiteman, 1994).…”

Section: Introductionmentioning

confidence: 99%

Ecological study of a bioaugmentation failure

Bouchez

Patureau

Dabert

et al. 2000

Environmental Microbiology

281

143

View full text Add to dashboard Cite

A nitrifying sequencing batch reactor was inoculated twice with the aerobic denitrifying bacterium Microvirgula aerodenitrificans and fed with acetate. No improvement was obtained on nitrogen removal. The second more massive inoculation was even followed by a nitrification breakdown, while at the same time, nitrification remained stable in a second reactor operated under the same conditions without bioaugmentation. Fluorescent in situ hybridization with rRNA-targeted probes revealed that the added bacteria almost disappeared from the reactor within 2 days, and that digestive vacuoles of protozoa gave strong hybridization signals with the M. aerodenitrificans-specific probe. An overgrowth of protozoa, coincident with the disappearance of free-living bacteria, was monitored by radioactive dot-blot hybridization only in the bioaugmented reactor. Population dynamics were analysed with a newly developed in situ quantification procedure of the probe-targeted bacteria. The nitrifying groups of bacteria decreased in a similar way in the bioaugmented and non-bioaugmented reactors. Other bacterial groups evolved differently. The involvement of different ecological parameters are discussed separately for each reactor. These results underline the importance of predator-prey interaction and illustrate the undesirable effects of massive bioaugmentation.

show abstract

“…F I G U R E 2 An unsolved problem related to exploiting bioremediation potential of Pseudomonas in phenol contaminated field environment highlighting the potential of filamentous phage to provide solutions and path of research to arrive at them (based on Goldstein, Mallory, & Alexander, 1985;Mrozik, Miga, & Piotrowska-Seget, 2011). FP, filamentous phage.…”

Section: Provide Nitrogenmentioning

confidence: 99%

“…Some researchers believe that stressful habitats favor toxin producers, which invade such habitats and outcompete the toxin-susceptible strains (Hibbing, Fuqua, Parsek, & Peterson, 2010;Majeed, Gillor, Kerr, & Riley, 2011;Riley & Gordon, 1999). In fact, the markedly lower biodegradation efficacy of bacterial inocula in vivo than that in in vitro has been attributed to their sensitivity to toxins produced by native bacteria (Goldstein et al, 1985;Mrozik et al, 2011).…”

Section: Phages To Make Beneficial Bacteria More Competitivementioning

confidence: 99%

Application of filamentous phages in environment: A tectonic shift in the science and practice of ecorestoration

Sharma

Karmakar

Kumar

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

Theories in soil biology, such as plant–microbe interactions and microbial cooperation and antagonism, have guided the practice of ecological restoration (ecorestoration). Below‐ground biodiversity (bacteria, fungi, invertebrates, etc.) influences the development of above‐ground biodiversity (vegetation structure). The role of rhizosphere bacteria in plant growth has been largely investigated but the role of phages (bacterial viruses) has received a little attention. Below the ground, phages govern the ecology and evolution of microbial communities by affecting genetic diversity, host fitness, population dynamics, community composition, and nutrient cycling. However, few restoration efforts take into account the interactions between bacteria and phages. Unlike other phages, filamentous phages are highly specific, nonlethal, and influence host fitness in several ways, which make them useful as target bacterial inocula. Also, the ease with which filamentous phages can be genetically manipulated to express a desired peptide to track and control pathogens and contaminants makes them useful in biosensing. Based on ecology and biology of filamentous phages, we developed a hypothesis on the application of phages in environment to derive benefits at different levels of biological organization ranging from individual bacteria to ecosystem for ecorestoration. We examined the potential applications of filamentous phages in improving bacterial inocula to restore vegetation and to monitor changes in habitat during ecorestoration and, based on our results, recommend a reorientation of the existing framework of using microbial inocula for such restoration and monitoring. Because bacterial inocula and biomonitoring tools based on filamentous phages are likely to prove useful in developing cost‐effective methods of restoring vegetation, we propose that filamentous phages be incorporated into nature‐based restoration efforts and that the tripartite relationship between phages, bacteria, and plants be explored further. Possible impacts of filamentous phages on native microflora are discussed and future areas of research are suggested to preclude any potential risks associated with such an approach.

show abstract

Reasons for possible failure of inoculation to enhance biodegradation

Cited by 320 publications

References 21 publications

Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal‐working fluid

Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal‐working fluid

Ecological study of a bioaugmentation failure

Application of filamentous phages in environment: A tectonic shift in the science and practice of ecorestoration

Contact Info

Product

Resources

About