Reassessment of Antigenic Determinant of <i>Saccharomyces cerevisiae</i> Serotype Ia

Nishikawa, Akemi; Sekine, Takeshi; Ikeda, Reiko; Saitô, Takako; Yoshimura, Fuminobu

doi:10.1111/j.1348-0421.1990.tb01061.x

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…The 1 H NMR spectrum of NF‐2‐1 was identical with the spectrum of the α‐Man‐(1→3)‐Man standard. The 1 H NMR spectra of both NF‐2–1 and NF‐1 were identical to previous data for α‐(1→3)‐linked mannobiose and mannotriose [10]. FACE analyses of the neutral oligosaccharide fractions are shown in Fig.…”

Section: Resultssupporting

confidence: 79%

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Structural studies of a cell wall polysaccharide of Trichosporon asahii containing antigen II

Ichikawa¹,

Nishikawa²,

Ikeda³

et al. 2001

European Journal of Biochemistry

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The structure of a cell-wall polysaccharide containing antigen II from Trichosporon asahii was investigated. A purified glucuronoxylomannan (GXM) antigen was found to contain O-acetyl groups that contribute to the serological reactivity. The structure of GXM was analyzed by partial acid hydrolysis, methylation analysis, controlled Smith degradation, NMR studies, and fluorophore-assisted carbohydrate electrophoresis. GXM has an a- (

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Section: Resultssupporting

confidence: 79%

“…The procedure described by Nishikawa et al . [10] was used. Factor II serum was prepared from anti‐( T. asahii ) serum by adsorption with T. cutaneum IFO 1198 (serotype I) and T. domesticum (serotype III).…”

Section: Methodsmentioning

confidence: 99%

Structural studies of a cell wall polysaccharide of Trichosporon asahii containing antigen II

Ichikawa¹,

Nishikawa²,

Ikeda³

et al. 2001

European Journal of Biochemistry

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“…Thus, it has long been thought that type 1 fimbriae of enterobacteria were functionally quite similar and that the primary essential characteristic of any potential receptor was the presence of terminal ot-3-linked mannosyl residues. (38). The majority of the carbohydrate of human plasma Fn is composed of N-glycosidic complex-type biantennary glycans, and no high-mannose-type or hybrid-type N-glycans have been described (30,45,54).…”

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confidence: 99%

FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes

et al. 1994

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We recently reported that the type 1-fimbriated Escherichia coli strains CSH-50 and HB101(pPKIA), both K-12 derivatives, have different patterns of adhesion to yeast mannan, human plasma fibronectin, and fibronectin derivatives, suggesting functional heterogeneity of type 1 fimbriae. In this report, we provide evidence that this functional heterogeneity is due to variations in the fimH genes. We also investigated functional heterogeneity among clinical isolates and whether variation infimH genes accounts for differences in receptor specificity. Twelve isolates obtained from human urine were tested for their ability to adhere to mannan, fibronectin, periodate-treated fibronectin, and a synthetic peptide copying the 30 amino-terminal residues of fibronectin. CSH-50 and HB101(pPKLA) were tested for comparison. Selected isolates were also tested for adhesion to purified fragments spanning the entire fibronectin molecule. Three distinct functional classes, designated M, MF, and MFP, were observed. The fimH genes were amplified by PCR from chromosomal DNA obtained from representative Strains and expressed in a Afim strain (AAEC191A) transformed with a recombinant plasmid containing the entire fim gene cluster but with a translational stop-linker inserted into the fimH gene (pPKL114). Cloned fimH genes conferred on AAEC191A(pPKL114) receptor specificities mimicking those of the parent strains from which the fimH genes were obtained, demonstrating that the FimH subunits are responsible for the functional heterogeneity. RepresentativefimH genes were sequenced, and the deduced amino acid sequences were compared with the previously published FimH sequence. Allelic variants exhibiting >98% homology and encoding proteins differing by as little as a single amino acid substitution confer distinct adhesive phenotypes. This unexpected adhesive diversity within the FimH family broadens the scope of potential receptors for enterobacterial adhesion and may lead to a fundamental change in our understanding of the role(s) that type 1 fimbriae may play in enterobacterial ecology or pathogenesis.

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“…Furthermore, the oligosaccharides synthesized by using enzymes of C. albicans and C. guilliermondii cells were labeled by the addition of small letters, a and g, respectively. Substrate oligosaccharides, Man␣132Man␣132Man␣132Man (AMan 4 ), and Man␣13 3Man␣132Man␣132Man␣132Man (AMan 5 ) were prepared from C. albicans mannan (24), Man␣133Man␣132Man␣132Man (SMan 4 ) and Man␣133Man␣133Man␣132Man␣132Man (SMan 5 ) were from S. cerevisiae serotype Ia mannan (27) 5 , and SMan 5 , respectively, using the ␣-1,6-mannosyltransferase of C. albicans NIH B-792 cells followed by the method described in a previous paper (23).…”

Section: Methodsmentioning

confidence: 99%

Characterization of β-1,2-Mannosyltransferase in Candida guilliermondii and Its Utilization in the Synthesis of Novel Oligosaccharides

Suzuki

Shibata

Suzuki

et al. 1997

Journal of Biological Chemistry

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A particulate insoluble enzyme fraction containing mannosyltransferases from Candida guilliermondii IFO 10279 strain cells was obtained as the residue after extracting a 105,000 ؋ g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Man␣133(Man␣136)Man␣132Man␣132Man, in the presence of GDP-mannose and Mn 2؉ ion at pH 6.0 gave a third type of ␤-1,2 linkage-containing mannohexaose, Man␤132Man␣133(Man␣136)Man␣132Man-␣132Man, the structure of which was identified by means of a sequential NMR assignment. The results of a substrate specificity study indicated that the ␤-1,2-mannosyltransferase requires a mannobiosyl unit, Man␣13 3Man␣13, at the nonreducing terminal site. We synthesized novel oligosaccharides using substrates possessing a nonreducing terminal ␣-1,3-linked mannose unit prepared from various yeast mannans. Further incubation of the enzymatically synthesized oligosaccharide with the enzyme fraction gave the following structure, Man␤132Man␤132Man␣133(Man␣136)Man␣13 2Man␣132Man, which has been found to correspond to antigenic factor 9. Incubation of Candida albicans serotype B mannan with the enzyme fraction gave significantly transformed mannan, which contains the third type of ␤-1,2-linked mannose units.

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Reassessment of Antigenic Determinant of Saccharomyces cerevisiae Serotype Ia

Cited by 15 publications

References 26 publications

Structural studies of a cell wall polysaccharide of Trichosporon asahii containing antigen II

Structural studies of a cell wall polysaccharide of Trichosporon asahii containing antigen II

FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes

Characterization of β-1,2-Mannosyltransferase in Candida guilliermondii and Its Utilization in the Synthesis of Novel Oligosaccharides

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