2014
DOI: 10.1089/ten.tea.2013.0269
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Recellularization of Well-Preserved Acellular Kidney Scaffold Using Embryonic Stem Cells

Abstract: For chronic kidney diseases, there is little chance that the vast majority of world's population will have access to renal replacement therapy with dialysis or transplantation. Tissue engineering would help to address this shortcoming by regeneration of damaged kidney using naturally occurring scaffolds seeded with precursor renal cells. The aims of the present study were to optimize the production of three-dimensional (3D) rat whole-kidney scaffolds by shortening the duration of organ decellularization proces… Show more

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Cited by 184 publications
(163 citation statements)
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“…Both kidney and liver bioreactors described here represent organ-specific refinements to improve recellularization and monitoring of cell growth and function based on the original cardiac perfusion model described by Langendorff 18,19 and later modified for the kidney 7,10,15,16 and liver 2,9,11,12,30 bioengineering. The modified kidney bioreactor design described here improves upon these models by incorporating both ureteral and arterial routes for recellularization allowing for a direct comparison, and we show improved recellularization using high-pressure arterial infusion compared to ureteral infusion of renal epithelial cells.…”
Section: Discussionmentioning
confidence: 99%
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“…Both kidney and liver bioreactors described here represent organ-specific refinements to improve recellularization and monitoring of cell growth and function based on the original cardiac perfusion model described by Langendorff 18,19 and later modified for the kidney 7,10,15,16 and liver 2,9,11,12,30 bioengineering. The modified kidney bioreactor design described here improves upon these models by incorporating both ureteral and arterial routes for recellularization allowing for a direct comparison, and we show improved recellularization using high-pressure arterial infusion compared to ureteral infusion of renal epithelial cells.…”
Section: Discussionmentioning
confidence: 99%
“…Specifically, given the inherent complexity of the renal architecture, kidney scaffold recellularization has been particularly challenging. 7,10,15,16 To overcome this limitation, we provide new detail on bioreactor features that allow for comparison of alternative seeding strategies and demonstrate improved renal epithelial cell dispersion throughout the tubular compartment of decellularized scaffolds.…”
Section: Introductionmentioning
confidence: 99%
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“…The goal of the described decellularization protocol is to produce an acellular renal ECM that can be used as a 3D scaffolding system for regeneration of kidney structures, including nephron tubules that are repopulated in the present example with human renal cortical tubular epithelial (RCTE) cells. We previously described our rigorous evaluation of an optimal, detergent-based rat kidney decellularization protocol 7 , which is more rapid (approximately one day) than other methods previously reported (Ross et ), and exposes the organ to a considerably lower concentration (0.1%) of the denaturant sodium dodecyl sulfate (SDS) during decellularization than prior reports [12][13][14][15] .…”
Section: Introductionmentioning
confidence: 99%
“…Recent advances in stem and progenitor cell technology have induced cells to differentiate into and produce tissue-appropriate cell compositions and ECM components and form biomimetic tissues with nascent functions, including the cerebral organoids (9). These technologies show self-organization capability of cells in tissue-mimetic environments, such as native tissue-derived decellularized scaffolds (10)(11)(12)(13). However, densely packed brain tissue with an architecture defined by neuronal connectivity (14,15) presents a unique challenge to define modular structures with specific functions.…”
mentioning
confidence: 99%