Recent achievements of bioluminescence imaging based on firefly luciferin-luciferase system

Li, Shufeng; Ruan, Zhiyang; Zhang, Hang; Xu, Haiwei

doi:10.1016/j.ejmech.2020.113111

Cited by 44 publications

(27 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, numerous luciferin analogues [63,64] and mutant luciferases [65][66][67][68][69][70] have been developed for high-resolution detection. A particularly important development in in vivo BLI technology is the development of luciferin analogues and mutant luciferases that provide near-infrared (NIR) wavelength luminescence.…”

Section: Renilla Reniformasmentioning

confidence: 99%

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

View full text Add to dashboard Cite

Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

show abstract

Section: Renilla Reniformasmentioning

confidence: 99%

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…In recent years, a number of bioluminescence probes, specifically firefly luciferin probes, have been reported. 11 It has been outlined that the 6′-site active hydrogen group and 4-carboxyl group of luciferins are essential for bioluminescence reactions. In addition, the caged firefly luciferin strategy is the main approach for designing probes.…”

Section: Introductionmentioning

confidence: 99%

Constructing firefly luciferin bioluminescence probes for in vivo imaging

Yang

Qin

et al. 2022

Org. Biomol. Chem.

View full text Add to dashboard Cite

show abstract

“…Firefly bioluminescence, light produced by the reaction of firefly luciferin (here, luciferin) and firefly luciferase (here, luciferase) (1), has for several decades been applied to optical imaging technology used in the field of life science (2,3). To detect numerous biological events with high sensitivity, various luciferin analogs and luciferase mutants have been developed recently (4)(5)(6). Especially, luciferin analogs that produce near-infrared (NIR) light (650-900 nm) are reported (7) because bio-tissues are more permeable to NIR light than visible light (8).…”

Section: Introductionmentioning

confidence: 99%

Absorption Spectra for Firefly Bioluminescence Substrate Analog: TokeOni in Various pH Solutions

Ogawa

Ono

Noguchi

et al. 2021

Photochem & Photobiology

View full text Add to dashboard Cite

AkaLumine hydrochloride, named TokeOni, is one of the firefly luciferin analogs, and its reaction with firefly luciferase produces near-infrared (NIR) bioluminescence. Prior to studying the bioluminescence mechanism, basic knowledge about the chemical structures, electronic states, and absorption properties of TokeOni at various pH values of solution has to be acquired. In this paper, the absorption spectra for TokeOni and AkaLumine at pH 2-10 were measured. Density functional theory (DFT) calculations, time-dependent DFT calculations, and the vibrational analyses were carried out. The absorption spectra indicate that the chemical forms of TokeOni in solutions are same as those of AkaLumine. The peaks at pH 7-10 in the absorption spectra correspond to the excitation from the ground state of a carboxylate anion of AkaLumine, the peak at pH 2 corresponds to the excitation from the ground state of a carboxylate anion with an Nprotonated thiazoline ring and N-protonated dimethylamino group of AkaLumine, and the peak at pH 4 corresponds to the excitation from the ground state of a carboxylate anion with an N-protonated thiazoline ring of AkaLumine.

show abstract

Recent achievements of bioluminescence imaging based on firefly luciferin-luciferase system

Cited by 44 publications

References 124 publications

How to Select Firefly Luciferin Analogues for In Vivo Imaging

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Constructing firefly luciferin bioluminescence probes for in vivo imaging

Absorption Spectra for Firefly Bioluminescence Substrate Analog: TokeOni in Various pH Solutions

Contact Info

Product

Resources

About