Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review

Abstract

Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is an urgent task for preventing disease outbreaks. Microfluidic devices are simple, automatic, and portable miniaturized systems. Compared with traditional techniques, microfluidic devices have attracted much attention because of their high efficiency and convenience in the concentration and detection of foodborne pathogens. This article firstly reviews the bio-recognition elements integrated on microfluidic chips in recent years and the progress of microfluidic chip development for pathogen pretreatment. Furthermore, the research progress of microfluidic technology based on optical and electrochemical sensors for the detection of foodborne pathogenic bacteria is summarized and discussed. Finally, the future prospects for the application and challenges of microfluidic chips based on biosensors are presented. Graphical abstract

Recent Progress in Spectroscopic Methods for the Detection of Foodborne Pathogenic Bacteria

“…The controlled continuous flow of microbes from the microfluidic channel helps in detecting the scattered light. Microfluidic chips are playing an essential role in the advancement of POCT devices [ 96 , 97 ]. An embedded microfluidic chip platform has been linked with optical fibers for connecting photosensors and laser light.…”

Advances in Organ-on-a-Chip Materials and Devices

“…Organ-on-a-chip (OoC) materials represent an emerging research field that has recently attracted substantial attention, because of their conceivable role in the development of microfluidic systems for physiological monitoring, precise diagnosis, drug discovery, etc. The recent research demonstrates a portable fluorescence resonance energy transfer (FRET)-based Pb-biosensor for environmental applications and microfluidic technology-based optical and electrochemical sensors for disease detection. − The detection of pathogenic bacteria, with diversified biosensors (such as electrochemical, optical, microfluidic, etc.) and signal amplification technologies (such as enzyme catalysis, nucleic acid chain reaction, biotin–streptavidin, click chemistry, cascade reaction, nanomaterials, etc.)…”

A novel observation platform for determining the micro-dispersion performance in practical reaction systems

“…Microreaction technology has evolved rapidly over the past decades with extensive applications in more and more fields, mainly due to the characteristics of fast mixing, advanced mass and heat transfer performances, and inherent safety. [1][2][3][4] This technology also provides a powerful tool especially for the development of high-temperature and high-pressure reactions, highly exothermic reactions, and reactions involving hazardous materials. [5][6][7][8] Due to the transformation from macro to micro scale, the working mechanisms in microdevices differ significantly from traditional large reactors.…”