Recent advancements in optofluidics-based single-cell analysis: optical on-chip cellular manipulation, treatment, and property detection

Huang, Nien-Tsu; Zhang, Hua Li; Chung, Meng Ting; Seo, Ji Min; Kurabayashi, Katsuo

doi:10.1039/c3lc51211h

Cited by 118 publications

(78 citation statements)

References 101 publications

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“…Microfluidic chips have been used for dispensing, 19 cell analyzing, 20 and cell sorting 21 as well as drug delivery. 22,23 In 2010, Marchington et al 24 reported automated optoinjection into cells by using a microfluidic chip fabricated in polydimethylsiloxane. Propidium iodide was used for the evaluation of molecular uptake.…”

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confidence: 99%

Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser

et al. 2016

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Abstract. The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and ex vivo gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.

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confidence: 99%

Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser

et al. 2016

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“…31 Lastly, the ability to manipulate individual trapped nanoparticles may permit sorting of individual particles based on measurement characteristics, an important but underexplored area. In spite of these unique features of optical tweezers, their low throughput characteristic 32,33 presents a major challenge in its development as a multiparametric characterization tool for nanoparticles. A high-throughput capability is practically necessary to improve the efficiency of data collection when working with samples containing a low concentration of nanoparticles such as HIV virions in human blood 34 or trying to detect rare particles such as titanium oxide (TiO 2 ) in the tertiary effluent of waste water plant.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic-based high-throughput optical trapping of nanoparticles

Kotnala

Zheng

et al. 2017

Lab Chip

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Optical tweezers have emerged as a powerful tool for multiparametric analysis of individual nanoparticles with single-molecule sensitivity. However, its inherent low-throughput characterstic remains a major obstacle for its applications within and beyond the laboratory. This limitation is further exacerbated when working with low concentration nanoparticle samples. Here, we present a microfluidic-based optical tweezers system that can ‘actively’ deliver nanoparticles to a designated microfluidic region for optical trapping and analysis. The active microfluidic delivery of nanoparticles results in significantly improved throughput and efficiency for optical trapping of nanoparticles. We observed a more than tenfold increase of optical trapping throughput for nanoparticles as compared to conventional systems at the same nanoparticle concentration. To demonstrate the utility of this microfluidic-based optical tweezers system, we further used the back-focal plane interferometry coupled with the trapping laser for precise quantitation of nanoparticle size without prior knowledge of the refractive index of nanoparticles. The development of this microfluidic-based active optical tweezers system thus opens the door to high-throughput multiparametric analysis of nanoparticles using precision optical trap in the future.

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“…There are four main approaches to cell manipulation: optical, magnetic, electric, and mechanical. [9][10][11][12][13] Of these, the mechanical approach of hydrodynamics is usually used for applications with large numbers of cells, or cells requiring fast manipulation because it is capable of driving the whole flow in a microfluidic device. In the mechanical approach of hydrodynamics, the cell motion is usually assumed to be moving with the flow of the suspension fluid, because of its low Reynolds number.…”

Section: Introductionmentioning

confidence: 99%

On-chip actuation transmitter for enhancing the dynamic response of cell manipulation using a macro-scale pump

et al. 2015

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An on-chip actuation transmitter for achieving fast and accurate cell manipulation is proposed. Instead of manipulating cell position by a directly connected macro-scale pump, polydimethylsiloxane deformation is used as a medium to transmit the actuation generated from the pump to control the cell position. This actuation transmitter has three main advantages. First, the dynamic response of cell manipulation is faster than the conventional method with direct flow control based on both the theoretical modeling and experimental results. The cell can be manipulated in a simple harmonic motion up to 130 Hz by the proposed actuation transmitter as opposed to 90 Hz by direct flow control. Second, there is no need to fill the syringe pump with the sample solution because the actuation transmitter physically separates the fluids between the pump and the cell flow, and consequently, only a very small quantity of the sample is required (<1 ll). In addition, such fluid separation makes it easy to keep the experiment platform sterilized because there is no direct fluid exchange between the sample and fluid inside the pump. Third, the fabrication process is simple because of the single-layer design, making it convenient to implement the actuation transmitter in different microfluidic applications. The proposed actuation transmitter is implemented in a lab-on-a-chip system for red blood cell (RBC) evaluation, where the extensibility of red blood cells is evaluated by manipulating the cells through a constriction channel at a constant velocity. The application shows a successful example of implementing the proposed transmitter. V C 2015 AIP Publishing LLC.

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Recent advancements in optofluidics-based single-cell analysis: optical on-chip cellular manipulation, treatment, and property detection

Cited by 118 publications

References 101 publications

Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser

Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser

Microfluidic-based high-throughput optical trapping of nanoparticles

On-chip actuation transmitter for enhancing the dynamic response of cell manipulation using a macro-scale pump

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