Recent advancements in various steps of ethanol, butanol, and isobutanol productions from woody materials

Fatehi, Pedram

doi:10.1002/btpr.1688

Cited by 35 publications

(25 citation statements)

References 110 publications

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“…Additionally, there are concerns related to the large‐scale utilization of petroleum fuels such as global warming and environmental pollution. In 2050, the world population is projected to increase up to 10 billion; therefore, this increase will significantly increase world‐wide energy needs …”

Section: Introductionmentioning

confidence: 99%

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Ethanol production from rice hull using Pichia stipitis and optimization of acid pretreatment and detoxification processes

Germeç

Kartal

Bilgic

et al. 2016

Biotechnology Progress

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The goal of this study was to produce ethanol from rice hull hydrolysates (RHHs) using Pichia stipitis strains and to optimize dilute acid hydrolysis and detoxification processes by response surface methodology (RSM). The optimized conditions were found as 127.14°C, solid:liquid ratio of 1:10.44 (w/v), acid ratio of 2.52% (w/v), and hydrolysis time of 22.01 min. At these conditions, the fermentable sugar concentration was 21.87 g/L. Additionally, the nondetoxified RHH at optimized conditions contained 865.2 mg/L phenolics, 24.06 g/L fermentable sugar, no hydroxymethylfurfural (HMF), 1.62 g/L acetate, 0.36 g/L lactate, 1.89 g/L glucose, and 13.49 g/L fructose + xylose. Furthermore, RHH was detoxified with various methods and the best procedures were found to be neutralization with CaO or charcoal treatment in terms of the reduction of inhibitory compounds as compared to nondetoxified RHH. After detoxification procedures, the content of hydrolysates consisted of 557.2 and 203.1 mg/L phenolics, 19.7 and 21.60 g/L fermentable sugar, no HMF, 0.98 and 1.39 g/L acetate, 0 and 0.04 g/L lactate, 1.13 and 1.03 g/L glucose, and 8.46 and 12.09 g/L fructose + xylose, respectively. Moreover, the base-line mediums (control), and nondetoxified and detoxified hydrolysates were used to produce ethanol by using P. stipitis strains. The highest yields except that of base-line mediums were achieved using neutralization (35.69 and 38.33% by P. stipitis ATCC 58784 and ATCC 58785, respectively) and charcoal (37.55% by P. stipitis ATCC 58785) detoxification methods. Results showed that the rice hull can be utilized as a good feedstock for ethanol production using P. stipitis. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:872-882, 2016.

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Section: Introductionmentioning

confidence: 99%

“…These sources consist of agricultural wastes, domestic wastes, agro‐industrial wastes, forest residuals, etc. and are referred to as renewable sources …”

Section: Introductionmentioning

confidence: 99%

Ethanol production from rice hull using Pichia stipitis and optimization of acid pretreatment and detoxification processes

Germeç

Kartal

Bilgic

et al. 2016

Biotechnology Progress

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“…Many options for removal of n ‐butanol have been reported in the literature, but the number of options studied for isobutanol is much lower. Adsorption, gas stripping, and pervaporation have all been evaluated for n ‐butanol. Below we summarize the main results from a separate paper that focused on the removal of isobutanol (6% (v/v)) from aqueous solution using pervaporation .…”

Section: Resultsmentioning

confidence: 99%

Towards cell-free isobutanol production: Development of a novel immobilized enzyme system

Grimaldi

Collins

Belfort

2015

Biotechnol Progress

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Producing fuels and chemical intermediates with cell cultures is severely limited by low product concentrations (≤0.2%(v/v)) due to feedback inhibition, cell instability, and lack of economical product recovery processes. We have developed an alternate simplified production scheme based on a cell-free immobilized enzyme system. Two immobilized enzymes (keto-acid decarboxylase (KdcA) and alcohol dehydrogenase (ADH)) and one enzyme in solution (formate dehydrogenase (FDH) for NADH recycle) produced isobutanol titers 8 to 20 times higher than the highest reported titers with S. cerevisiae on a mol/mol basis. These high conversion rates and low protein leaching were achieved by covalent immobilization of enzymes (ADH) and enzyme fusions (fKdcA) on methacrylate resin. The new enzyme system without in situ removal of isobutanol achieved a 55% conversion of ketoisovaleric acid to isobutanol at a concentration of 0.135 (mole isobutanol produced for each mole ketoisovaleric acid consumed). Further increasing titer will require continuous removal of the isobutanol using an in situ recovery system.

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“…Many options for removal of n‐butanol have been reported, but the number of recovery methods studied for isobutanol is much lower. Adsorption, gas stripping, and pervaporation have all been evaluated for n‐butanol.…”

Section: Resultsmentioning

confidence: 99%

Toward cell‐free biofuel production: Stable immobilization of oligomeric enzymes

Grimaldi¹,

Collins²,

Belfort³

2014

Biotechnology Progress

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To overcome the main challenges facing alcohol-based biofuel production, we propose an alternate simplified biofuel production scheme based on a cell-free immobilized enzyme system. In this paper, we measured the activity of two tetrameric enzymes, a control enzyme with a colorimetric assay, β-galactosidase, and an alcohol-producing enzyme, alcohol dehydrogenase, immobilized on multiple surface curvatures and chemistries. Several solid supports including silica nanoparticles (convex), mesopourous silica (concave), diatomaceous earth (concave), and methacrylate (concave) were examined. High conversion rates and low protein leaching was achieved by covalent immobilization of both enzymes on methacrylate resin. Alcohol dehydrogenase (ADH) exhibited long-term stability and over 80% conversion of aldehyde to alcohol over 16 days of batch cycles. The complete reaction scheme for the conversion of acid to aldehyde to alcohol was demonstrated in vitro by immobilizing ADH with keto-acid decarboxylase free in solution.

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Recent advancements in various steps of ethanol, butanol, and isobutanol productions from woody materials

Cited by 35 publications

References 110 publications

Ethanol production from rice hull using Pichia stipitis and optimization of acid pretreatment and detoxification processes

Ethanol production from rice hull using Pichia stipitis and optimization of acid pretreatment and detoxification processes

Towards cell-free isobutanol production: Development of a novel immobilized enzyme system

Toward cell‐free biofuel production: Stable immobilization of oligomeric enzymes

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