Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Abstract

Molecular diagnosis, which plays a major role in infectious disease screening with successful understanding of the human genome, has attracted more attention because of the outbreak of COVID-19 recently. Since point-of-care testing (POCT) can expand the application of molecular diagnosis with the benefit of rapid reply, low cost, and working in decentralized environments, many researchers and commercial institutions have dedicated tremendous effort and enthusiasm to POCT-based biosensing for molecular diagnosis. In this review, we firstly summarize the state-of-the-art techniques and the construction of biosensing systems for POC molecular diagnosis. Then, the application scenarios of POCT-based biosensing for molecular diagnosis were also reviewed. Finally, several challenges and perspectives of POC biosensing for molecular diagnosis are discussed. This review is expected to help researchers deepen comprehension and make progresses in POCT-based biosensing field for molecular diagnosis applications.

Diagnostic Accuracy of Liquid Biomarkers in Airway Diseases: Toward Point-of-Care Applications

“…The access of multiplex POC technology will be a gamechanger for chronic and complex disease diagnosis and monitoring. Unfortunately, a drawback of multiplex devices is that they are much more susceptible to cross-contamination, which could severely impair the accuracy of the result ( 171 ). To address this, device architecture can be modified with, for example, hydrophobic barriers that ensure reagents and analytes are restricted to flow along a defined path only that prevents mixing ( 172 , 173 ).…”

Mapping the Potential of Microfluidics in Early Diagnosis and Personalized Treatment of Head and Neck Cancers

“…Common biosensing strategies used in these devices can be categorized into three major groups based on their detection principle: electrochemical, optical, and mechanical sensing [68]. Enzyme-Linked Immunosorbent Assay (ELISA), Polymerization Chain Reaction (PCR), Surface-Enhanced Raman scattering (SERS), fluorescence-enabled and microbead-based assays are some of the major techniques routinely used for diagnostic and screening application of molecular analytes [69,70]. Detailed descriptions of these biosensing principles and their applications as POC devices can be found here [68,69].…”

Construction of a desirable hyperbolic microfluidic chip for ultrasensitive determination of PCT based on chemiluminescence

“…Generally, PCT can reflect the activity of systemic inflammation reactions, which was a parameter of diagnosis and monitoring bacterial inflammatory disease infection. [33][34][35] Therefore, the DHMC integration with chemiluminescence as signal readout enabled achieving the point-of-care testing (POCT) of PCT in the clinical diagnosis treatment (Scheme 1). Notably, the DHMC by simulation showed a high mixing efficiency for sample reagents, which can then improve the detection sensitivity.…”