Recent Advances in Affinity Capillary Electrophoresis (2007)

Liu, Xiaojun; Dahdouh, Froseen; Salgado, Marisol; Gomez, Frank A.

doi:10.1002/jps.21452

Cited by 48 publications

(33 citation statements)

References 61 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Affinity capillary electrophoresis (ACE) is a version of CE, and has been used to examine various biological interactions [18][19][20][21][22]. ACE using carbohydrate recognition proteins, such as lectins has certain advantages in the glycobiology era.…”

Section: Partial Filling Affinity Capillary Electrophoresis For Glycamentioning

confidence: 99%

Highly Sensitive Methods Using Liquid Chromatography and Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans

Suzuki

2014

Chromatography

View full text Add to dashboard Cite

This review summarizes our recent works concerned on the analysis of glycoprotein glycans. Glycoprotein glycans are highly complicated and have variations that number into the thousands. Therefore the method for their analyses should be carefully chosen according to the complexity and quantity of glycans in samples. To shorten the time for the preparation of labeled glycans from glycoprotein, we proposed two methods: PNGase F/NBD-F method enables the preparation of fluorescently labeled glycans from glycoproteins about 2 hr. A combination of a neutrally coated capillary and borate buffer eliminates the purification steps for the removal of excess reagents from the glycan samples for capillary electrophoresis. Partial filling affinity capillary electrophoresis using glycan-recognizing proteins such as lectins, glycosidases and immunoglobulins, was used for the structural analysis of glycans. The method was applied to the specific detection of NeuGc and α-Gal containing glycans known as the heterogenic antigens. Sensitivity of the detection of glycoprotein glycans separated by microchip-based electrophoresis was enhanced by in situ fabricated sufonate-type polyacrylamide gel. This method enhances the sensitivity by a factor of 10 5 . In situ fabrication of lectin impregnated gels enables specific entrapment and analysis of glycans. Conversion reaction from fluorescently labeled glycans (1-amino-1-alditols) to free oligosaccharides will be helpful for the functional analysis of glycans.

show abstract

Section: Partial Filling Affinity Capillary Electrophoresis For Glycamentioning

confidence: 99%

Highly Sensitive Methods Using Liquid Chromatography and Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans

Suzuki

2014

Chromatography

View full text Add to dashboard Cite

show abstract

“…Una variante de la ECA, es la EC de inmunoafinidad (ECIA) que contribuye notablemente al diagnóstico clínico y biomédico colocándose por encima de técnicas como el RIA (radio inmuno análisis) y ELISA (ensayo inmuno enzimático) 45,49 . Entre sus aplicaciones se encuentran la detección de enfermedades infecciosas (enfermedad de Chagas, tuberculosis, lepra, el virus del Nilo y el virus de inmunodeficiencia humana (VIH), entre otras) 61 , así como la determinación de metabolitos farmacéuticos (que pueden inducir daño orgánico, por ejemplo en riñón), de fármacos (metanfetaminas) y esteroides (testosterona) en orina a través de anticuerpos, elevando la sensibilidad, además de permitir un diagnóstico fino y un monitoreo eficaz de los agentes terapéuticos 4,59 .…”

Section: La Electroforesis Capilar De Afinidad (Eca)unclassified

“…En la presente era postgenómica, la velocidad para mejorar el conocimiento de las enfermedades humanas (especialmente las enfermedades multifactoriales), ha aumentado en forma considerable. La información acumulada gracias a la EC comienza a vislumbrar las causas genéticas de muchas enfermedades, fortaleciendo el diagnósti-co y reemplazando muchas de las metodologías clásicas para el estudio de la medicina genómica 4 .…”

unclassified

La electroforesis capilar como una nueva estrategia en la medicina y el diagnóstico clínico

Magaña

Arenas-Sordo

Gómez³

2009

Rev. méd. Chile

View full text Add to dashboard Cite

show abstract

“…CE appeared in the 1980s and was rapidly developed due to transition to the silica capillaries with very small and regular diameters (tens of micrometers) and application of the straight detection in the fluid flow [22]. The CE and related electroseparation techniques are widely used for the estimation of stability constants because of rapidity, low cost of reagents, and high performance [23][24][25][26][27][28][29][30][31]. To study the interactions between a metal and ligand, several electropherograms are recorded with varying ligand content in background electrolytes (BGE) and metal solution injected as a sample.…”

Section: Introductionmentioning

confidence: 99%

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

Sursyakova

Burmakina

Rubaylo

2016

Journal of Coordination Chemistry

View full text Add to dashboard Cite

Fax: 73912494108 2 Composition and stability constants of copper(II) complexes with succinic acid ions determined by capillary electrophoresisThe advantage of usage of capillary electrophoresis technique was demonstrated for studying a complicated system owing to the dependence of direction and velocity of the electrophoretic movement on the charge of complex species. The stability constants of copper(II) complexes with ions of succinic acid were determined by capillary electrophoresis, including the 1:2 metal to ligand complexes which are rarely mentioned and taken into account in studies during recent years. The measurements were carried out at 25 ºC and ionic strength of 0.1, obtained by mixing the solutions of succinic acid and lithium hydroxide up to рН 4.2 -6.2. It was shown that while pH was more than 4.5 the zone of copper(II) complexes with succinate ions moves as an anion. It is impossible to treat this fact using only the complexes with a metal-ligand ratio of 1:1 (CuL 0 , CuHL + ).The following values of stability constants were obtained: log β(CuL) = 2.89 ± 0.02, log β(CuHL + ) = 5.4 ± 0.5, log β(CuL 2 2-) = 3.88 ± 0.05, log β(CuHL 2 -) = 7.2 ± 0.3.

show abstract

Recent Advances in Affinity Capillary Electrophoresis (2007)

Cited by 48 publications

References 61 publications

Highly Sensitive Methods Using Liquid Chromatography and Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans

Highly Sensitive Methods Using Liquid Chromatography and Capillary Electrophoresis for Quantitative Analysis of Glycoprotein Glycans

La electroforesis capilar como una nueva estrategia en la medicina y el diagnóstico clínico

Composition and stability constants of copper(II) complexes with succinic acid determined by capillary electrophoresis

Contact Info

Product

Resources

About