Recent advances in amino acid analysis by CE

Poinsot, Véréna; Gavard, Pierre; Feurer, Bernard; Couderc, FranÃ§ois

doi:10.1002/elps.200900399

Cited by 65 publications

(32 citation statements)

References 107 publications

(106 reference statements)

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“…and derivatising agent (dansyl chloride, phenylisothiocyanate, ophthalaldehyde, fluorenylchloroformate analogues, etc.) [24]. However, most of these methods are not optimal for several reasons: for example, GC requires a complex derivatisation step, since the analytes are not sufficiently volatile to be analysed as such [11]; ion-exchange systems usually require dedicated systems; reversed-phase HPLC requires gradient elution.…”

Section: Introductionmentioning

confidence: 98%

Fast analysis of amino acids in wine by capillary electrophoresis with laser‐induced fluorescence detection

et al. 2011

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A fast analytical method has been developed for the determination of nine amino acids, together with serotonin, in wine samples of different origin and vintage. The method is based on capillary electrophoresis coupled to laser-induced fluorescence detection. Separation was obtained by using a fused-silica capillary (75 μm id, 74.0 cm total length, 60.0 cm length to detector) and a background electrolyte composed of carbonate buffer (20 mM, pH 9.2), applying a 20 kV voltage. Direct hydrodynamic injection of wine samples was made after an original microwave-assisted derivatisation step with 5-(4,6-dichlorotriazinyl)aminofluorescein. Fluorescence was induced by an Ar-Ion laser, exciting at 488 nm. Good linearity (r(2) >0.9990) was obtained for all considered analytes and sensitivity was also good, with limits of detection in the 7-50 ng/mL range. The method was successfully applied for the analysis of commercial Italian wines and thus seems to be suitable for the determination of the relevant amino acids and serotonin, providing good results in terms of accuracy and precision, together with the advantage of a very fast, microwave-assisted derivatisation procedure. Future applications of the method are planned to check for wine adulterations and commercial frauds.

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Section: Introductionmentioning

confidence: 98%

Fast analysis of amino acids in wine by capillary electrophoresis with laser‐induced fluorescence detection

et al. 2011

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“…The suitability of capillary zone electrophoresis (CZE) for the analysis of amino acids and peptides, in general, is demonstrated in current reviews [13,14]. Also larger thiolic peptides, such as different types of phytochelatines, can be separated by means of CZE [15].…”

Section: Introductionmentioning

confidence: 99%

CZE of Sulfur-Containing Amino Acids and Peptides and Its Application to the Quantitative Study of Heavy Metal-Caused Thiol Oxidations

2012

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The redox-active, sulfur-containing amino acids cysteine and methionine, the tripeptide glutathione and their oxidized counterparts cystine, methionine sulfoxide, and glutathione disulfide were separated as anions by capillary zone electrophoresis (CZE) in a 72 cm long fused silica capillary filled with 100 mM phosphate buffer, pH 8.0, at a voltage of ?30 kV in 20 min. The optimized CZE method was suited for the implementation of quantitative metal interaction studies of the biomolecules in a biologically relevant concentration range (lM-mM). Decreasing peak areas of the reduced forms of cysteine and glutathione and simultaneously increasing peak areas of the oxidized forms after incubation of the reduced biomolecules with divalent heavy metal cations indicated redox reactions which could be responsible for toxic metal actions in biological systems. CZE measurements revealed that a 50 % oxidation grade of cysteine was achieved at a molar metal:cysteine ratio of 0.85 in case of Zn(II) addition and of 0.11 in case of Cu(II) addition, respectively. Cu(II) oxidized 50 % of the initial glutathione at a molar Cu:peptide ratio of 0.036, whereas the 50 % oxidation grade was not reached after incubation with Co(II) up to a molar ratio of Co:peptide of 0.25.

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“…Over the past decade, microfluidic chip has emerged as an attractive method for chemical and biological analysis using miniaturized systems [1][2][3]. Recent applications of microfluidic chip-based analytical tools tend toward the integration of multiple unit processes such as sample pretreatment, separation, and detection into a single chip.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic device with compound structure

Zhang

Liu

et al. 2013

2013 International Conference on Optoelectronics and Microelectronics (ICOM)

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The development of microfluidic chip has become a growing research field. Accompanied by the new polymer materials, the preparation process and the function of the microfluidic chip is becoming diversification. In this paper we propose a novel method for preparing microfluidic amperometric detection chip using the printed circuit board (PCB). The chip is consists of three parts: the printed circuit board substrate with micro-strip electrodes, the polymethyl methacrylate (PMMA) with microchannels and the PDMS as assistant bonding layer. The electrode size can achieve micron level and the detection electrodes which were integrated on the PCB is not easy to fall off or broken. The microfluidic chip would be manufactured numerously while reduce costs by using the mature PCB production process.

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Recent advances in amino acid analysis by CE

Cited by 65 publications

References 107 publications

Fast analysis of amino acids in wine by capillary electrophoresis with laser‐induced fluorescence detection

Fast analysis of amino acids in wine by capillary electrophoresis with laser‐induced fluorescence detection

CZE of Sulfur-Containing Amino Acids and Peptides and Its Application to the Quantitative Study of Heavy Metal-Caused Thiol Oxidations

Microfluidic device with compound structure

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