Recent advances in colorimetric strategies for acetylcholinesterase assay and their applications

Liu, Dongmei; Xu, Bingjie; Dong, Chuan

doi:10.1016/j.trac.2021.116320

Cited by 87 publications

(44 citation statements)

References 120 publications

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“…The Coomassie brilliant blue method was used to measure the yield of immobilized LDH [29]. One hundred µL of the LDH solution before and after immobilization was added to 5 mL Coomassie brilliant blue solution, reacted at room temperature for 10 min, and the absorbance was then measured at 595 nm.…”

Section: Relative Activity (%) =mentioning

confidence: 99%

Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Extract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles

Zhang¹,

Li²,

Liu³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Lactate dehydrogenase (LDH) is one of the important enzyme systems for glycolysis and gluconeogenesis. It can catalyze the reduction and oxidation reaction between propionic acid and L-lactic acid, which is usually overexpressed in cancer cells. Therefore, inhibiting the activity of LDH is a promising way for the treatment of cancer. In this study, an effective method based on ligand fishing and ultra performance liquid chromatography-mass spectrum (UPLC-MS) was established to screen and identify active ingredients from Selaginella doederleinii with potential inhibitory activity for LDH. Methods: Firstly, LDH was immobilized on the magnetic nanoparticles (MNPs), three immobilization parameters including LDH concentration, immobilization time and pH were optimized by single factor and response surface methodology for maximum (max) immobilization yield. Then, a mixed model of galloflavin and chlorogenic acid (inhibitors and non-inhibitors of LDH) was used to verify the specificity of immobilized LDH ligand fishing, and the conditions of ligand fishing were further optimized. Finally, combined with UPLC-MS, immobilized LDH was used to simultaneously screen and identify potential LDH inhibitors from the ethyl acetate extract of Selaginella doederleinii. Results: The prepared fishing material was comprehensively characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD) and fourier transform infrared spectrometer (FT-IR). The optimal immobilization conditions were obtained as LDH concentration of 0.7 mg/mL, pH value of 4.5, and immobilization time of 3.5 h. Under these conditions, the max immobilization yield was (3.79 ± 0.08) × 10 3 U/g. The specificity analysis showed that immobilized LDH could recognize and capture ligands, and the optimal ligand fishing conditions included that the incubation time was 30 min, the elution time was 20 min, and the concentration of methanol as eluent was 80%. Finally, two LDH inhibitors, amentoflavone and robustaflavone, were screened by immobilized LDH from the ethyl acetate extract of Selaginella doederleinii. Conclusions: The study provided a meaningful evidence for discovering the bioactive constituents in ethyl acetate extract of Selaginella doederleinii related to cancer treatment, and this ligand fishing method was feasible for screening enzyme inhibitors from similar complex mixtures.

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Section: Relative Activity (%) =mentioning

confidence: 99%

Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Extract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles

Zhang¹,

Li²,

Liu³

et al. 2022

Front. Biosci. (Landmark Ed)

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“…Attributed to the attractive features of easy readout and real-time visual detection via naked eyes, the colorimetric sensing mode attracted wide attention in the field of detecting pesticides and related biomarkers [37,38]. In a typical ChE-CHO-nanozyme colorimetric platform, chromogenic substrates including TMB, ABTS, and OPD are usually used to be catalyzed by an HRP-like nanozyme with the participation of H 2 O 2 to produce color signals.…”

Section: Ternary Che-cho-nanozyme Assaysmentioning

confidence: 99%

“…Zhu's group prepared a peroxidase-like SAN with high-concentration Cu sites on carbon nanosheets and constructed an AChE-CHO-SAN cascade reaction system for the colorimetric detection of ACh and OPs [48]. Attributed to the attractive features of easy readout and real-time visual detection via naked eyes, the colorimetric sensing mode attracted wide attention in the field of detecting pesticides and related biomarkers [37,38]. In a typical ChE-CHO-nanozyme colorimetric platform, chromogenic substrates including TMB, ABTS, and OPD are usually used to be catalyzed by an HRP-like nanozyme with the participation of H2O2 to Although these ChE-CHO-nanozyme colorimetric assays exhibit a variety of merits and were widely applied for pesticide determination, most of them are performed in solution, which are inefficient and complex to operate for in-field detection.…”

Section: Ternary Che-cho-nanozyme Assaysmentioning

confidence: 99%

“…To highlight the progress in this field, here we intend to summarize the principles, methods, and applications of nanozyme-based sensors for pesticide and ChE detection. What should be stated is that although there are a number of thematic reviews on pesticide and related biomarker biosensing [15,[37][38][39][40][41][42][43][44], no reviews focus on the emerging applications of nanozymes in pesticide biosensors. Our contribution is expected to fill in the gap.…”

Section: Introductionmentioning

confidence: 99%

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Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Zhu

Liu

et al. 2021

Biosensors

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To improve the output and quality of agricultural products, pesticides are globally utilized as an efficient tool to protect crops from insects. However, given that most pesticides used are difficult to decompose, they inevitably remain in agricultural products and are further enriched into food chains and ecosystems, posing great threats to human health and the environment. Thus, developing efficient methods and tools to monitor pesticide residues and related biomarkers (acetylcholinesterase and butylcholinesterase) became quite significant. With the advantages of excellent stability, tailorable catalytic performance, low cost, and easy mass production, nanomaterials with enzyme-like properties (nanozymes) are extensively utilized in fields ranging from biomedicine to environmental remediation. Especially, with the catalytic nature to offer amplified signals for highly sensitive detection, nanozymes were finding potential applications in the sensing of various analytes, including pesticides and their biomarkers. To highlight the progress in this field, here the sensing principles of pesticides and cholinesterases based on nanozyme catalysis are definitively summarized, and emerging detection methods and technologies with the participation of nanozymes are critically discussed. Importantly, typical examples are introduced to reveal the promising use of nanozymes. Also, some challenges in the field and future trends are proposed, with the hope of inspiring more efforts to advance nanozyme-involved sensors for pesticides and cholinesterases.

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“…6 AChE has become a vital biomarker and drug target in clinical diagnosis and detoxification therapy. Thus, the accurate assaying of AChE activity is highly significant from the clinical point of view, and various analytical methods have been established for this purpose, including Ellman's colorimetric, 7,8 chemiluminescent 9,10 and electrochemical approaches. 11,12 The treatment of real biological samples, however, is always highly challenging due to the interference from biomolecules and the instability of reagents.…”

mentioning

confidence: 99%

MnO₂/DNAzyme-mediated ratiometric fluorescence assay of acetylcholinesterase

Zhang

Wang

et al. 2022

Analyst

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Recent advances in colorimetric strategies for acetylcholinesterase assay and their applications

Cited by 87 publications

References 120 publications

Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Extract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles

Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Extract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

MnO₂/DNAzyme-mediated ratiometric fluorescence assay of acetylcholinesterase

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Recent advances in colorimetric strategies for acetylcholinesterase assay and their applications

Cited by 87 publications

References 120 publications

Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Extract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles

Lactate Dehydrogenase-Inhibitors Isolated from Ethyl Acetate Extract of Selaginella doederleinii by Using a Rapid Screening Method with Enzyme-Immobilized Magnetic Nanoparticles

Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

MnO2/DNAzyme-mediated ratiometric fluorescence assay of acetylcholinesterase

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MnO₂/DNAzyme-mediated ratiometric fluorescence assay of acetylcholinesterase