Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals

Zhang, Yu; Zhang, Rui; Yang, Xiaolin; Qi, Honglan; Zhang, Chengxiao

doi:10.1016/j.jpha.2018.11.004

Cited by 77 publications

(32 citation statements)

References 99 publications

(191 reference statements)

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“…Chemiluminescence (CL) is a practical opto-chemical approach for designing real-life sensing applications, e.g., safety control within food and pharmaceutical industries, environmental hazardous contaminants monitoring and clinical examination, using the discharged radiation for high sensitivity analysis of the target molecule [13][14][15][16][17][18]. The addition of superparamagnetism characteristics onto CL bioassays in means of magnetic nanoparticles (MNPs), alleviate the high-throughput immunoassay protocols by convenient separation, widespread the linear sensing range, enhance the reaction kinetics and encompass high surface area for collected biomolecular recognition [19].…”

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confidence: 99%

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

et al. 2020

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Background: Haptoglobin is an acute-phase protein used as predicting diagnostic biomarker both in humans (i.e., diabetes, ovarian cancer, some neurological and cardiovascular disorders) and in animals (e.g., bovine mastitis). The latter is a frequent disease of dairy industry with staggering economical losses upon decreased milk production and increased health care costs. Early stage diagnosis of the associated diseases or inflammation onset is almost impossible by conventional analytical manners. Results:The present study demonstrates a simple, rapid, and cost-effective label-free chemiluminescence bioassay based on magnetite nanoparticles (MNPs) for sensitive detection of haptoglobin by employing the specific interaction of hemoglobin-modified MNPs. The resulting haptoglobin-hemoglobin complex inhibits the peroxidase-like activity of luminol/H 2 O 2 -hemoglobin-MNPs sensing scheme and reduces the chemiluminescence intensities correspondingly to the innate haptoglobin concentrations. Quantitative detection of bovine haptoglobin was obtained within the range of 1 pg mL −1 to 1 µg mL −1 , while presenting 0.89 pg mL −1 limit of detection. Moreover, the influence of causative pathogenic bacteria (i.e., Streptococcus dysgalactiae and Escherichia coli) and somatic cell counts (depicting healthy, sub-clinical and clinical mastitis) on the emitted chemiluminescence radiation were established. The presented bioassay quantitative performances correspond with a standardized assay kit in differentiating dissimilar milk qualities. Conclusions:Overall, the main advantage of the presented sensing concept is the ability to detect haptoglobin, at clinically relevant concentrations within real milk samples for early bio-diagnostic detection of mastitis and hence adjusting the precise treatment, potentially initiating a positive influence on animals' individual health and hence on dairy farms economy.

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confidence: 99%

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

et al. 2020

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“…After pretreatment and digestion, quantitative MS analysis is carried out [ 47 ]. Another conventional method for determining serum mAb concentrations is immunoassay including the enzyme-linked immunosorbent assay (ELISA) [ 48 ] and electrochemiluminescence immunoassay (ECLIA) [ 49 , 50 ]; however, they may suffer from lower precision (about 10% of relative standard deviation in repeatability) and robustness for quantification purposes [ 11 ].…”

Section: Assessment Of Biological Functionsmentioning

confidence: 99%

Recent progress in the molecular imaging of therapeutic monoclonal antibodies

Zeng

Qian

2020

Journal of Pharmaceutical Analysis

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Therapeutic monoclonal antibodies have become one of the central components of the healthcare system and continuous efforts are made to bring innovative antibody therapeutics to patients in need. It is equally critical to acquire sufficient knowledge of their molecular structure and biological functions to ensure the efficacy and safety by incorporating new detection approaches since new challenges like individual differences and resistance are presented. Conventional techniques for determining antibody disposition including plasma drug concentration measurements using LC-MS or ELISA, and tissue distribution using immunohistochemistry and immunofluorescence are now complemented with molecular imaging modalities like positron emission tomography and near-infrared fluorescence imaging to obtain more dynamic information, while methods for characterization of antibody’s interaction with the target antigen as well as visualization of its cellular and intercellular behavior are still under development. Recent progress in detecting therapeutic antibodies, in particular, the development of methods suitable for illustrating the molecular dynamics, is described here.

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“…Electrodes based on metals, semiconductors and carbon derivatives have gained great importance in recent years in the development of sensors based on MIPs. Although their use has always been relegated to the development of electrochemical sensors it is possible to use them as optical sensors due to electrochemiluminescence (ECL) [85], a kind of luminescence in which radiative emission is obtained from an electrochemical reaction in solution. This method requires no irradiation and problems such as scattering or light source instability are avoided.…”

Section: Bi-dimensional Compositesmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Hybrid Materials for the Development of Optical Sensors

Rico-Yuste¹,

Carrasco

2019

Polymers

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We report on the development of new optical sensors using molecularly imprinted polymers (MIPs) combined with different materials and explore the novel strategies followed in order to overcome some of the limitations found during the last decade in terms of performance. This review pretends to offer a general overview, mainly focused on the last 3 years, on how the new fabrication procedures enable the synthesis of hybrid materials enhancing not only the recognition ability of the polymer but the optical signal. Introduction describes MIPs as biomimetic recognition elements, their properties and applications, emphasizing on each step of the fabrication/recognition procedure. The state of the art is presented and the change in the publication trend between electrochemical and optical sensor devices is thoroughly discussed according to the new fabrication and micro/nano-structuring techniques paving the way for a new generation of MIP-based optical sensors. We want to offer the reader a different perspective based on the materials science in contrast to other overviews. Different substrates for anchoring MIPs are considered and distributed in different sections according to the dimensionality and the nature of the composite, highlighting the synergetic effect obtained as a result of merging both materials to achieve the final goal.

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Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals

Cited by 77 publications

References 99 publications

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

Recent progress in the molecular imaging of therapeutic monoclonal antibodies

Molecularly Imprinted Polymer-Based Hybrid Materials for the Development of Optical Sensors

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