2021
DOI: 10.1016/j.ccr.2020.213613
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Recent advances in fluorescent probes for cellular antioxidants: Detection of NADH, hNQO1, H2S, and other redox biomolecules

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Cited by 84 publications
(32 citation statements)
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“…The most common and typical methods, such as high-performance liquid chromatography (HPLC) and inductively coupled plasma-optical emission spectroscopy/mass spectrometry (ICP-OES/MS), have been developed for the determination of small biomolecules and ions in vitro [ 6 8 ]. Onsite determination of these analytes in situ and in vivo, using these techniques, is not possible because the sample preparation in solution is an essential step to ensure performing successful analysis [ 9 ]. Imaging technologies, such as computerized tomography (CT) [ 10 ], magnetic resonance imaging (MRI) [ 11 ] and positron emission tomography (PET), have been widely used in clinical diagnosis [ 12 ], while these technologies cannot be directly used for the determination of the concentration and/or activity of these analytes in biological samples [ 13 , 14 ].…”
Section: Introductionmentioning
confidence: 99%
“…The most common and typical methods, such as high-performance liquid chromatography (HPLC) and inductively coupled plasma-optical emission spectroscopy/mass spectrometry (ICP-OES/MS), have been developed for the determination of small biomolecules and ions in vitro [ 6 8 ]. Onsite determination of these analytes in situ and in vivo, using these techniques, is not possible because the sample preparation in solution is an essential step to ensure performing successful analysis [ 9 ]. Imaging technologies, such as computerized tomography (CT) [ 10 ], magnetic resonance imaging (MRI) [ 11 ] and positron emission tomography (PET), have been widely used in clinical diagnosis [ 12 ], while these technologies cannot be directly used for the determination of the concentration and/or activity of these analytes in biological samples [ 13 , 14 ].…”
Section: Introductionmentioning
confidence: 99%
“…Although some probes have been developed to detect reductive substances associated with epileptic seizures, it is difficult for the concentration of bioactive molecules detected in vitro to reflect their true levels in living organisms, due to their high reactive activities. , In vivo fluorescence imaging has been a direct approach to monitor biomarkers in living bodies. However, there is still a lack of a general design strategy for in situ fluorescence monitoring of the antioxidant response in epileptic brains. For example, although many fluorescent probes have been reported to detect hydrogen sulfide (H 2 S) in living cells and in vivo, few fluorescent probes have been used for the detection and imaging of H 2 S in the brain, especially for in situ monitoring of H 2 S fluctuation during epileptic seizures. This is mainly because of the challenges to cross the blood–brain barrier (BBB) and the low penetration of short-wavelength light. …”
Section: Introductionmentioning
confidence: 99%
“…To better understand the signaling or regulatory function of H 2 S n , it is highly urgent to develop a new analytical tool that allows researchers to track the formation and consumption of this RSS species in vivo . Fluorescent probes possessing the advantages of simple operation, fast detection speed, and noninvasiveness are available for real-time bioimaging. However, it has been a long-term difficulty to construct reversibly responsive fluorescent probes for monitoring H 2 S n , especially near-infrared (NIR)-excitable ones, which can obviously increase imaging depth and alleviate autofluorescence from biosamples. At present, fluorescent probes for specifically detecting H 2 S n are mainly based on two strategies. One is dependent on the reducibility of H 2 S n , which enables to result in the transformation of nitrobenzene into aniline to eliminate the fluorescence quenching caused by photoinduced electron transfer (PET). The other strategy takes advantage of its nucleophilic nature to release fluorophores through substitution-cyclization with 2-fluoro-5-nitrobenzoic ester or phenyl 2-(benzoylthio)­benzoate et al, boosting fluorescence through the enhanced intramolecular charge transfer (ICT) effect. To obtain a reversible probe for tracking H 2 S n level in real time, the recognition reaction should have a proper reaction activity to ensure the high reaction rate and selectivity, and an appropriate dissociation constant to release the captured H 2 S n under the condition of a low concentration of H 2 S n .…”
Section: Introductionmentioning
confidence: 99%