Recent advances in natural products exploitation in Streptomyces via synthetic biology

Abstract

Natural products of microbial origin have proven to be the wellspring of clinically useful compounds for human therapeutics. Streptomyces species are predominant sources of bioactive compounds, most of which serve as potential drug candidates. While the exploitation of natural products has been severely reduced over the past two decades, the growing crisis of evolution and dissemination of drug resistant pathogens have again attracted great interest in this field. The emerging synthetic biology has been heralded as a new bioengineering platform to discover novel bioactive compounds and expand bioactive natural products diversity and production. Herein, we review recent advances in the natural products exploitation of Streptomyces with the applications of synthetic biology from three major aspects, including recently developed synthetic biology tools, natural products biosynthetic pathway engineering strategies as well as chassis host modifications.

Recent Advances in Silent Gene Cluster Activation in Streptomyces

“…All in all, thorough reconstruction of BGCs often leads to the activation of silent BGCs and the discovery of new NPs ( Luo et al, 2016 ; Zhao et al, 2019 ). However, compared with E. coli and other model strains, the genetic manipulation tools in Streptomyces are still limited.…”

Synthetic biology approaches to actinomycete strain improvement

“… 2019 ; Palazzotto et al . 2019 ; Zhao, Wang and Luo 2019 ). In this mini review, we highlight selected areas of synthetic biology as applied to actinomycete strain improvement.…”

Jejucarbazoles A–C, carbazole glycosides with indoleamine 2,3-dioxygenase 1 inhibitory activity fromStreptomycessp. KCB15JA151

“…Natural products from actinomycetes have been an excellent source for drug discovery due to their diversity of carbon skeletons and biological activities. [1][2][3][4] Natural products can not only be used as drugs but also provide drug leads for the development of new drugs. [3][4][5][6] A substantial number of natural product derived drugs have been released on the market.…”