Recent Advances in Orchid Seed Germination and Micropropagation

Li, Yuanyuan; Chan, C. L.; Stahl, C. F.; Yeung, Edward C.

doi:10.1007/978-1-4939-7771-0_27

Cited by 8 publications

(5 citation statements)

References 111 publications

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“…There are other factors that should be considered in the optimization of a micropropagation protocol. For example, the composition of the culture, orchids, as epiphytes, require low-nitrate culture media, as is the case of the VW medium (Li et al 2018). However, in our study, G. skinneri showed a better development in MS medium combined with TIS.…”

Section: Discussioncontrasting

confidence: 50%

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

et al. 2020

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This study's aim was to establish a protocol for the micropropagation of G. skinneri using temporary immersion system (TIS). Different concentrations of 6-Benzylaminopurine (0, 1, 2, and 3 mg L −1 ), three different systems of cultivate semi-solid (SS) and liquid media under partial (PI) and temporary immersion systems (TIS), different compositions of the inorganic salts, and the number of subcultures were evaluated. The results showed a maximum of 16.56 shoots per explant obtained through TIS, adjusting all the parameters evaluated in our study. One higher number of shoots per explant was observed in the micropropagation of G. skinneri TIS compared to SS and PI. While the use of 3 mg L −1 of BAP + MS (Murashige and Skoog) media was better than 3 mg L −1 of BAP VW (Vacint and Went) for the generation of a greater number of shoots per explant, 6.33 and 2.72, respectively. The immersion frequency of 2 min every 4 h allowed the production to be scaled to 8.54 shoots per explant. While it was necessary to perform three subcultures every 30 days, to obtain 16.56 shoots per explant, a rooting phase was not required due to the generation of adventitious roots during the different subcultures. However, a phase of elongation of the regenerated plants with ½ MS + GA3 (gibberellic acid) was needed to guarantee 100% survival in the process of acclimatization. In conclusion, this plant production system can be applied for the commercial micropropagation of this species for ornamental purposes, as well as for its reintroduction in protected natural areas.

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Section: Discussioncontrasting

confidence: 50%

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

et al. 2020

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“…Asymbiotic seed germination has been applied to reproduce several orchid species and seeks to emulate the nutrition obtained by the mycorrhizal fungi under in vitro conditions (Dutra et al 2008;Dutra et al 2009). Several culture media have been tested and usually vary in the chemical composition and concentration of several micronutrients and natural compound extracts (Fay 1992;Li et al 2018). Furthermore, the use of phytohormones as growth stimulators has been reported (Romero et al 2017).…”

Section: Asymbiotic Seed Germinationmentioning

confidence: 99%

Orchid Mycorrhizal Interactions on the Pacific Side of the Andes from Chile. A Review

Herrera

García-Romera

Meneses

et al. 2019

J Soil Sci Plant Nutr

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In order to confront the constant decline in global biological diversity, amelioration strategies are needed for threatened species to design reintroduction policies, particularly in plants with critical reproduction steps, such as orchids. Orchids are part of a highly diverse plant family, with several species under imminent extinction risk. This is the case of Chilean Orchidaceae, which has shown a constant decay in their populations due to an increase in the alteration processes of their natural distribution habitats. Successful orchid reintroductions require a full understanding of orchid mycorrhizal fungi and their dynamic according to different developmental stages and environmental conditions because orchid seeds need mycorrhizal fungi to obtain nutritional compounds at early developmental stages. This article performed a critical literature review of the ecological studies conducted on Chilean orchids and their relationships with mycorrhizal fungi in order to focus on the best scientific approach to achieve successful restoration programs involving orchid seeds and compatible mycorrhizal fungi.

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“…It has been shown that plant cells have a higher ability to metabolize and transfer nitrogen from organic rather than inorganic sources [29]. Casein hydrolysate has been reported to be effective in various plant developmental processes including somatic embryogenesis [30,31], seed germination and seedling growth [32], and callus proliferation [33]. Additionally, the positive role of casein hydrolysate, as a reduced nitrogen form in vitro shoot regeneration of many plant species has been documented previously [34][35][36].…”

Section: Discussionmentioning

confidence: 97%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

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Recent Advances in Orchid Seed Germination and Micropropagation

Cited by 8 publications

References 111 publications

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

Orchid Mycorrhizal Interactions on the Pacific Side of the Andes from Chile. A Review

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

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