Recent advances in periodontal regeneration: A biomaterial perspective

Liang, Yongxi; Luan, Xianghong; Liu, Xiaohua

doi:10.1016/j.bioactmat.2020.02.012

Cited by 193 publications

(209 citation statements)

References 170 publications

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“…In recent years, different combinations of scaffolds and growth factors have been applied to the field of periodontal regeneration to repair the periodontium and recover its function (Gümüşderelioglu et al, 2019;Liang et al, 2020). Despite of the achievements in past decades, further studies are FIGURE 10 | ALP activity of hPDLCs (× 100).…”

Section: Discussionmentioning

confidence: 99%

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Liao

Shu

et al. 2020

Front. Cell. Infect. Microbiol.

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The recovery of impaired periodontium is still a challenge to the treatment of periodontitis. This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration. The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration. The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis. Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo. The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 µg/mL rhAm. Tryptic Soy Broth culture medium was used as a baseline control. Osteogenic effect of rhAm (20 µg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 µg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media. The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control. In the animal model, 4week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo. The root slices were treated with rhAm (20 µg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 µg/mL rhAm). The root slices treated with osteogenic medium alone were used as the baseline control. The analyses showed that the mHA/CS particles were 2 µm in diameter and had a uniform pore size. Liao et al. A Research on Periodontal Regeneration The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m 2 /g. The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis. The mHA/CS scaffold sustainably released rhAm. The mHA/CS loaded with 20 µg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro. Additionally, it promoted the formation of cementum-like tissue in vivo. Our findings suggest that mHA/CS loaded with 20 µg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.

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Section: Discussionmentioning

confidence: 99%

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Liao

Shu

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Although various attempts to augment oral soft tissues using xenogeneic materials have been reported, no ideal substitute material is currently considered to be available and predictable for periodontal and peri-implant plastic surgical reconstructions [ 1 ]. The use of xenogeneic materials is of particular advantage in situations where autologous transplants cannot be harvested in sufficient amount or quality, e.g., in thin biotype, shallow palate, or covering of multiple recessions [ 2 , 3 ]. In addition, autogenous soft tissue grafting procedures are usually associated with significant morbidity at the patient donor site [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

et al. 2020

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Xenogeneic acellular collagen matrices represent a safe alternative to autologous soft tissue transplants in periodontology and implant dentistry. Here, we aimed to investigate the adsorption and release of growth factors from four porcine-derived collagen matrices using enzyme-linked immunosorbent assay. Non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) adsorbed each of the following growth factors, TGF-β1, FGF-2, PDGF-BB, GDF-5 and BMP-2, with an efficiency close to 100%. Growth factor release for a 13-day period was in the range of 10–50% of the adsorbed protein, except for the BMP-2 release that was in the range of 5–7%. Generally, protein release occurred in two phases. Phase I was arbitrary defined by the highest release from the matrices, usually within 24 h. Phase II, spanning the period immediately after the peak release until day 13, corresponded to the delayed release of the growth factors from the deeper layers of the matrices. HADM showed significantly (P < 0.001) higher TGF-β1, FGF-2, and PDGF-BB release in phase II, compared to the rest of the matrices. NCM exhibited significantly (P < 0.001) higher FGF-2 release in phase II, compared to CCM and DADM as well as a characteristic second peak in PDGF-BB release towards the middle of the tested period. In contrast to NCM and HADM, CCM and DADM showed a gradual and significantly higher release of GDF-5 in the second phase. Several burst releases of BMP-2 were characteristic for all matrices. The efficient adsorption and sustained protein release in the first 13 days, and the kinetics seen for HADM, with a burst release within hours and high amount of released growth factor within a secondary phase, may be beneficial for the long-term tissue regeneration following reconstructive periodontal surgery.

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“…Each layer of the structure was printed continuously, following the geometry edge of the designed cylinder with a single extruded filament. Based on the recommendations and findings of recent studies [ 8 , 12 , 39 ], in order to increase pore interconnectivity (with pore dimensions between 150 and 500 μm) without compromising the mechanical properties, and allow a better in vitro performance, an offset distance equal to half the distance between strands was used (see Figure 1 ). Specifically, for the first 9 layers of the scaffold, intended to mimic the alveolar bone, the distance between two adjoining strands was set as 0.8 mm.…”

Section: Methodsmentioning

confidence: 99%

“…Bone tissue augmentation is a procedure of paramount importance to provide mechanical stability and aesthetic functionality, before dental implant placement into patients. Augmentation can be achieved through the use of different bone grafting materials (including autografts, allografts and xenografts) and with properties that vary from space maintenance, blood clot stabilization and scaffolding, by providing a temporary template to support tissue regeneration [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the mechanical performance, the scaffold should possess adequate mechanical strength until full tissue formation is achieved. Moreover, and in order to guarantee its function, the scaffold should degrade at a rate that has to match the bone remodeling process, which in the case of an alveolar bone within 5–6 months has been considered optimal [ 8 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Towards 3D Multi-Layer Scaffolds for Periodontal Tissue Engineering Applications: Addressing Manufacturing and Architectural Challenges

et al. 2020

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Reduced periodontal support, deriving from chronic inflammatory conditions, such as periodontitis, is one of the main causes of tooth loss. The use of dental implants for the replacement of missing teeth has attracted growing interest as a standard procedure in clinical practice. However, adequate bone volume and soft tissue augmentation at the site of the implant are important prerequisites for successful implant positioning as well as proper functional and aesthetic reconstruction of patients. Three-dimensional (3D) scaffolds have greatly contributed to solve most of the challenges that traditional solutions (i.e., autografts, allografts and xenografts) posed. Nevertheless, mimicking the complex architecture and functionality of the periodontal tissue represents still a great challenge. In this study, a porous poly(ε-caprolactone) (PCL) and Sr-doped nano hydroxyapatite (Sr-nHA) with a multi-layer structure was produced via a single-step additive manufacturing (AM) process, as a potential strategy for hard periodontal tissue regeneration. Physicochemical characterization was conducted in order to evaluate the overall scaffold architecture, topography, as well as porosity with respect to the original CAD model. Furthermore, compressive tests were performed to assess the mechanical properties of the resulting multi-layer structure. Finally, in vitro biological performance, in terms of biocompatibility and osteogenic potential, was evaluated by using human osteosarcoma cells. The manufacturing route used in this work revealed a highly versatile method to fabricate 3D multi-layer scaffolds with porosity levels as well as mechanical properties within the range of dentoalveolar bone tissue. Moreover, the single step process allowed the achievement of an excellent integrity among the different layers of the scaffold. In vitro tests suggested the promising role of the ceramic phase within the polymeric matrix towards bone mineralization processes. Overall, the results of this study demonstrate that the approach undertaken may serve as a platform for future advances in 3D multi-layer and patient-specific strategies that may better address complex periodontal tissue defects.

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Recent advances in periodontal regeneration: A biomaterial perspective

Cited by 193 publications

References 170 publications

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

Towards 3D Multi-Layer Scaffolds for Periodontal Tissue Engineering Applications: Addressing Manufacturing and Architectural Challenges

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