Recent advances in production and extraction of bacterial lipids for biofuel production

Hwangbo, Myung; Chu, Kung-Hui

doi:10.1016/j.scitotenv.2020.139420

Cited by 42 publications

(22 citation statements)

References 123 publications

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“…In general, to overcome drawbacks associated with solvent extraction, different strategies have been applied to oleaginous microorganisms integrating solvent extraction with mechanical processes. However, all of them usually demand high-cost technologies [ 97 ]. Recently, innovative methods based on the application of lytic enzymes or bacteriophages have been developed to enable cell disruption and release of intracellular components, including the accumulated oils [ 96 , 97 , 98 ].…”

Section: Conversion Of Industrial Wastes Into Microbial Oils By Rhodococcimentioning

confidence: 99%

Rhodococcus as Biofactories for Microbial Oil Production

et al. 2021

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Bacteria belonging to the Rhodococcus genus are frequent components of microbial communities in diverse natural environments. Some rhodococcal species exhibit the outstanding ability to produce significant amounts of triacylglycerols (TAG) (>20% of cellular dry weight) in the presence of an excess of the carbon source and limitation of the nitrogen source. For this reason, they can be considered as oleaginous microorganisms. As occurs as well in eukaryotic single-cell oil (SCO) producers, these bacteria possess specific physiological properties and molecular mechanisms that differentiate them from other microorganisms unable to synthesize TAG. In this review, we summarized several of the well-characterized molecular mechanisms that enable oleaginous rhodococci to produce significant amounts of SCO. Furthermore, we highlighted the ability of these microorganisms to degrade a wide range of carbon sources coupled to lipogenesis. The qualitative and quantitative oil production by rhodococci from diverse industrial wastes has also been included. Finally, we summarized the genetic and metabolic approaches applied to oleaginous rhodococci to improve SCO production. This review provides a comprehensive and integrating vision on the potential of oleaginous rhodococci to be considered as microbial biofactories for microbial oil production.

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Section: Conversion Of Industrial Wastes Into Microbial Oils By Rhodococcimentioning

confidence: 99%

Rhodococcus as Biofactories for Microbial Oil Production

et al. 2021

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“…Despite the extraction being initiated from dry or wet biomass, each has its own advantages and disadvantages. Most importantly is to develop scalable mechanical lipid extraction methods with low energy consumption [63][64][65]. The comparison of energy consumption of several mechanical pretreatment/cell disruption methods quantified for various oleaginous yeasts is shown in Table 2.…”

Section: Pretreatment/cell Disruption Of Oleaginous Yeast To Extract mentioning

confidence: 99%

Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

et al. 2021

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The production of lipids from oleaginous yeasts involves several stages starting from cultivation and lipid accumulation, biomass harvesting and finally lipids extraction. However, the complex and relatively resistant cell wall of yeasts limits the full recovery of intracellular lipids and usually solvent extraction is not sufficient to effectively extract the lipid bodies. A pretreatment or cell disruption method is hence a prerequisite prior to solvent extraction. In general, there are no recovery methods that are equally efficient for different species of oleaginous yeasts. Each method adopts different mechanisms to disrupt cells and extract the lipids, thus a systematic evaluation is essential before choosing a particular method. In this review, mechanical (bead mill, ultrasonication, homogenization and microwave) and nonmechanical (enzyme, acid, base digestions and osmotic shock) methods that are currently used for the disruption or permeabilization of oleaginous yeasts are discussed based on their principle, application and feasibility, including their effects on the lipid yield. The attempts of using conventional and “green” solvents to selectively extract lipids are compared. Other emerging methods such as automated pressurized liquid extraction, supercritical fluid extraction and simultaneous in situ lipid recovery using capturing agents are also reviewed to facilitate the choice of more effective lipid recovery methods.

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“…TAGs are energy-rich compounds formed by a molecule of glycerol esterified with three fatty acids chains. They can be used for biofuel generation after bacterial cell extraction through conventional chemical and physical extraction methods (e.g., sonication, solvent extraction) or through biological approaches (e.g., enzymatic cell lysis, phage-based extraction) (Hwangbo and Chu 2020).…”

Section: Introductionmentioning

confidence: 99%

Systems biology and metabolic engineering of Rhodococcus for bioconversion and biosynthesis processes

2021

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Rhodococcus spp. strains are widespread in diverse natural and anthropized environments thanks to their high metabolic versatility, biodegradation activities, and unique adaptation capacities to several stress conditions such as the presence of toxic compounds and environmental fluctuations. Additionally, the capability of Rhodococcus spp. strains to produce high value-added products has received considerable attention, mostly in relation to lipid accumulation. In relation with this, several works carried out omic studies and genome comparative analyses to investigate the genetic and genomic basis of these anabolic capacities, frequently in association with the bioconversion of renewable resources and low-cost substrates into triacylglycerols. This review is focused on these omic analyses and the genetic and metabolic approaches used to improve the biosynthetic and bioconversion performance of Rhodococcus. In particular, this review summarizes the works that applied heterologous expression of specific genes and adaptive laboratory evolution approaches to manipulate anabolic performance. Furthermore, recent molecular toolkits for targeted genome editing as well as genome-based metabolic models are described here as novel and promising strategies for genome-scaled rational design of Rhodococcus cells for efficient biosynthetic processes application.

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Recent advances in production and extraction of bacterial lipids for biofuel production

Cited by 42 publications

References 123 publications

Rhodococcus as Biofactories for Microbial Oil Production

Rhodococcus as Biofactories for Microbial Oil Production

Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

Systems biology and metabolic engineering of Rhodococcus for bioconversion and biosynthesis processes

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