Recent advances in single-molecule sequencing

Treffer, Regina; Deckert, Volker

doi:10.1016/j.copbio.2010.02.009

Cited by 52 publications

(32 citation statements)

References 52 publications

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“…For example, the high spatial resolution of TERS allowed researchers to resolve individual nucleobases on both RNA and DNA strands. [46,47] It was demonstrated that all four nucleobases could be identified on both RNA and DNA strands. [48,49] Recently, Lipiec et al reported that, using TERS, molecular structure of terminal groups at DNA fragments can be probed, which consequently allowed the authors to identify double strand breaks of DNA.…”

Section: Applications Of Ters In Materials and Life Sciencesmentioning

confidence: 99%

Tip-enhanced Raman spectroscopy: From concepts to practical applications

Jiang

Kurouski

Pozzi

et al. 2016

Chemical Physics Letters

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Tip-enhanced Raman spectroscopy (TERS) is a powerful technique that integrates the vibrational fingerprinting of Raman spectroscopy and the sub-nanometer resolution of scanning probe microscopy (SPM). As a result, TERS is capable of obtaining chemical maps of analyzed specimens with exceptional lateral resolution. This is extremely valuable for the study of interactions between molecules and substrates, in addition to structural characterization of biological objects, such as viruses and amyloid fibrils, 2D polymeric materials, and monitoring electrochemical and photo-catalytic processes. In this mini-review, we discuss the most significant advances of TERS, including: super high resolution chemical imaging, monitoring of catalytic processes, incorporation of pulsed-excitation techniques, single-site electrochemistry, biosensing, and art conservation. We begin with a short overview of TERS, comparing it with other surface analytical techniques, followed by an overview of recent developments and future applications in TERS.Graphical Abstract:

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Section: Applications Of Ters In Materials and Life Sciencesmentioning

confidence: 99%

Tip-enhanced Raman spectroscopy: From concepts to practical applications

Jiang

Kurouski

Pozzi

et al. 2016

Chemical Physics Letters

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“…17 On the other hand, in phyisiological conditions the DNA motion might even be too fast to detect the single bases at all. 18 In this work, we consider an alternative approach based on the thermoelectric properties of DNA codon units forming a molecular junction. Since the molecule is fixed at the ends, the aforementioned kinematic drawbacks are properly circumvented in these case.…”

Section: Discussionmentioning

confidence: 99%

Codon thermoelectric signature in molecular junctions

Maciá

2010

Phys. Rev. B

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The thermoelectric power of trimer oligonucleotides connected in between metallic contacts at different temperatures is theoretically studied. The obtained analytical expressions reveal the existence of important resonance effects leading to a significant thermopower enhancement for certain characteristic energies which depend on the specific electronic structure of considered codons. This result suggests the existence of a thermoelectric signature for different triplet associations of biological interest.

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“…Labeled single-stranded DNA sequences can be further used as detection probes in order to detect specific DNA sequences [94]. Specific hybridization of the probes (in accord with the rules of complementarity) to the DNA molecules under analysis puts up the platform for the detection of the latter based on the Raman signature of the dye [94,95].…”

Section: Serrs: the Ultimate Redressive Actionmentioning

confidence: 99%

Raman Spectroscopy: A Promising Technique for Analyzing Nucleic Acid Refractory to PCR Amplification

Matar¹,

Merheb²

2014

TOSPECJ

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Ancient DNA (aDNA) refers to the genetic material found in dead paleontological and archeological samples. Being subject to various types of stressors, it undergoes different hydrolytic and oxidative post mortem modifications that result in the formation of DNA lesions. These lesions are found to either block the DNA polymerase during replication or induce nucleotide misincorporations. Besides, aDNA samples occur in minimal quantities; which represents an additional obstacle that researchers have to overcome in order to study aDNA. The earliest major efforts included the use of molecular cloning and polymerase chain reaction (PCR) to amplify aDNA sequences. These techniques were later found to be associated with a number of false results and unauthentic findings. There have been numerous attempts to eliminate the shortcomings of PCR and improve the quality of aDNA through avoiding contamination, repairing lesions, using translesion polymerases, etc. However, the majority of these have failed to yield accurate and specific results. Surfaceenhanced resonance Raman scattering (SERRS), on the other hand, starts in the right foot by introducing a sensitive nonenzymatic approach for the specific detection of single-and double-stranded DNA. The ability of this method to evade DNA degradation is particularly important for not only studying aDNA, but also analyzing DNA refractory to PCR amplification in processed products.

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Recent advances in single-molecule sequencing

Cited by 52 publications

References 52 publications

Tip-enhanced Raman spectroscopy: From concepts to practical applications

Tip-enhanced Raman spectroscopy: From concepts to practical applications

Codon thermoelectric signature in molecular junctions

Raman Spectroscopy: A Promising Technique for Analyzing Nucleic Acid Refractory to PCR Amplification

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