Recent Advances in Small Molecule Microarrays: Applications and Technology

Walsh, Daniel P.; Chang, Y T

doi:10.2174/1386207043328427

Cited by 35 publications

(16 citation statements)

References 5 publications

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“…In the last few years, chemical microarrays were successfully used to obtain insights on protein-small molecule interactions, study enzyme inhibition, elucidate signal pathways and identify targets through libraries screening [62,[102][103][104][105].…”

Section: Small Moleculesmentioning

confidence: 99%

Advances in Parallel Screening of Drug Candidates

Bergese¹,

Cretich²,

Oldani³

et al. 2008

CMC

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In the hit to lead process, a drug candidate is selected from a set of potential leads by screening its binding with potential targets. This review focuses on the lead identification assays that employ a bio-chemical or bio-physical test to detect molecular recognition events between proteins and small molecules in a parallel format. These tests require either the lead or the target immobilization followed by incubation with the set of potential interaction partners and detection of a signal related to the target-ligand binding. In the first part of the review the different detection strategies amenable for drug screening are discussed. In the second part, a review of immobilization approaches for leads or targets, allowing the parallel screening of arrays of molecules, is presented.

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Section: Small Moleculesmentioning

confidence: 99%

Advances in Parallel Screening of Drug Candidates

Bergese¹,

Cretich²,

Oldani³

et al. 2008

CMC

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“…it is transparent to light, it has been frequently used for fluorescent bioassays for biological studies (protein, DNA microchips, etc. …) 206,207 and to prove energy transfer by assembly of donor and acceptor chromophores as mixed monolayers. 208,209 The first effective sensing systems using covalently bonded dyes to glass were used for pH sensing.…”

Section: Glass and Gold Surfacesmentioning

confidence: 99%

Design of Fluorescent Materials for Chemical Sensing

2007

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There is an enormous demand for chemical sensors for many areas and disciplines. High sensitivity and ease of operation are two main issues for sensor development. Fluorescence techniques can easily fulfill these requirements and therefore fluorescent-based sensors appear as one of the most promising candidates for chemical sensing. However, the development of sensors is not trivial; material science, molecular recognition and device implementation are some of the aspects that play a role in the design of sensors. The development of fluorescent sensing materials is increasingly captivating the attention of the scientists because its implementation as a truly sensory system is straightforward. This critical review shows the use of polymers, sol-gels, mesoporous materials, surfactant aggregates, quantum dots, and glass or gold surfaces, combined with different chemical approaches for the development of fluorescent sensing materials. Representative examples have been selected and they are commented here.

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“…Several probe discoveries enabled by SMMs have been recently reviewed (Hong et al, 2014). The process for manufacturing microarrays also has been reviewed previously (Shi et al, 2010; Uttamchandani et al, 2004; Uttamchandani and Yao, 2010; Vegas and Koehler, 2010; Walsh and Chang, 2004) and is not the focus of this protocol. The reader is referred to previous step-by-step protocols focused on the manufacture of SMMs (Bradner et al, 2006; Casalena et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Probing Small‐Molecule Microarrays with Tagged Proteins in Cell Lysates

Pop

Wassaf

Koehler

2014

CP Chemical Biology

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The technique of small‐molecule microarray (SMM) screening is based on the ability of small molecules to bind to various soluble proteins. This type of interaction is easily detected by the presence of a fluorescence signal produced by labeled antibodies that specifically recognize a unique sequence (tag) present on the target protein. The fluorescent signal intensity values are determined based on signal‐to‐noise ratios (SNRs). SMM screening is a high‐throughput, unbiased method that can rapidly identify novel direct ligands for various protein targets. This binding‐based assay format is generally applicable to most proteins, but it is especially useful for protein targets that do not possess an enzymatic activity. SMMs enable screening a protein in a purified form or in the context of a cellular lysate, likely providing a more physiologically relevant screening environment. © 2014 by John Wiley & Sons, Inc.

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Recent Advances in Small Molecule Microarrays: Applications and Technology

Cited by 35 publications

References 5 publications

Advances in Parallel Screening of Drug Candidates

Advances in Parallel Screening of Drug Candidates

Design of Fluorescent Materials for Chemical Sensing

Probing Small‐Molecule Microarrays with Tagged Proteins in Cell Lysates

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