Recent advances in the high performance liquid chromatography of lipids

Shukla, V. K. S.

doi:10.1016/0163-7827(88)90003-3

Cited by 58 publications

(9 citation statements)

References 176 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NPLC consists of polar stationary phases and non-polar mobile phases, while RPLC consists of non-polar stationary phases and polar mobile phases. RPLC is the dominating separation technique in lipidomics [71]. The separation mechanism in RPLC depends on the hydrophobic interaction between the solute molecule in the mobile phase and the hydrophobic stationary phase.…”

Section: Reversed Phase Lcmentioning

confidence: 99%

Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

Zeng

Araujo²,

Du³

et al. 2010

Talanta

View full text Add to dashboard Cite

Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS(2)) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS(2) for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.

show abstract

Section: Reversed Phase Lcmentioning

confidence: 99%

Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

Zeng

Araujo²,

Du³

et al. 2010

Talanta

View full text Add to dashboard Cite

show abstract

“…These results suggest that the effect of double bonds is minimal at >214 nm. Shukla (1988) also investigated this problem using triglycerides that had different fatty acid compositions with different degrees of unsaturation. They found that the double bonds disturbed the quantification of triglycerides at <218 nm, but quantification of triglycerides could be achieved at 220 nm, largely because of the ester C=O functional group.…”

Section: Hplc Of the Gl Fractionmentioning

confidence: 99%

“…The limitations of a UV or a refractive index detector have been overcome by the development of an evaporative light-scattering detector (ELSD). The advantages of an ELSD include gradient elution and analysis at relatively high temperatures, which decreases the unstable detection caused by the fluctuation of ambient temperature (Shukla 1988). Using an ELSD, Christie (1985) separated lipid classes from animal tissue and Christie and Morrison (1988) separated GL classes from wheat flour lipids.…”

mentioning

confidence: 99%

Estimation of Free Glycolipids in Wheat Flour by HPLC

Ohm

Chung

1999

Cereal Chem

View full text Add to dashboard Cite

The chloroform‐acetone mixture (4:1, v/v) was an effective solvent for eluting the nonpolar lipid fraction, including free fatty acids, from the polar lipid (glycolipid and phospholipid) fractions from free lipids of 21 hard winter wheat flours using a solid‐phase extraction system. Amounts of monogalactosyldiglycerides (MGDG) and digalactosyldiglycerides (DGDG) in the glycolipid fraction were determined by normal‐phase HPLC with a gradient system using an evaporative light‐scattering detector (ELSD) and a diode array detectors (DAD). Unsaturated fatty acids showed higher UV absorbances from 200 to 213 nm when compared with saturated palmitic acid. However, significant linear correlation coefficients were obtained between the peak areas measured by a DAD and GL contents determined by an ELSD, suggesting that fatty acid composition of flour GL could be fairly constant. Using an ELSD as a reference, equations for determination of MGDG or DGDG quantities were derived from the peak areas of a DAD by multivariate regression methods. Determination of MGDG and DGDG quantities was also possible using only a DAD.

show abstract

“…Compared with GC, HPLC can provide several advantages: (1) HPLC is the method that allows the nonvolatile thermally labile compounds to be separated with mild operating conditions; (2) detectors used in HPLC are nondestructive for the analytes, which makes it possible to collect the fraction for further investigation; (3) HPLC method allows FA to be converted to a large number of different derivatives. In addition, with the availability of various solvents, column packing materials and strong ultraviolet-absorbing or fluorescent tagging probes, a highly selective and sensitive HPLC analysis is possible (Perret et al, 2004;Puttmann, Krug, Von Ochsenstein, & Kattermann, 1993;Shukla, 1988). Commonly, chemical derivatization with fluorescence detection has been widely adopted in order to reduce detection limits.…”

Section: Introductionmentioning

confidence: 98%