Recent Advances in the Understanding of Molecular Mechanisms of Resistance in Noctuid Pests

Goff, Gaëlle Le; Nauen, Ralf

doi:10.3390/insects12080674

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…8 Among these two mechanisms, metabolic resistance is a more common mechanism with the increased detoxification enzymes such as cytochrome P450 monooxygenases (P450s), glutathione S -transferases (GSTs), or transporters like ATP binding cassette (ABC) transporters. 13 Recently, overexpression of GSTs and ABC genes was involved in insecticide resistance in C. pomonella . 8, 10 Besides, the increased production of P450s has been linked to resistance to insecticides including lambda -cyhalothrin in field-evolved resistant populations from Spain, France, Greece, Turkey, and China.…”

Section: Introductionmentioning

confidence: 99%

Metabolic functional redundancy of the CYP9A subfamily members leads to P450-mediated lambda-cyhalothrin resistance in Cydia pomonella

Li¹,

Shi

Liu³

et al. 2022

Preprint

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BACKGROUND: The evolution of insect resistance to pesticides poses a continuing threat to sustainable pest management. While much is known about the molecular mechanisms that confer resistance in model insects and few agricultural pests, far less is known about fruit pests. RESULTS: Here we found that functional redundancy and preference of metabolism by cytochrome P450 monooxygenases (P450s) genes in the CYP9A subfamily confer resistance to lambda-cyhalothrin in Cydia pomonella, a major invasive pest of pome fruit. A total of four CYP9A genes, including CYP9A61, CYP9A120, CYP9A121, and CYP9A122, were identified from C. pomonella. Among these, CYP9A120, CYP9A121, and CYP9A122 were predominantly expressed in the midgut of larvae. The expression levels of these P450 genes were significantly induced by LD10 of lambda-cyhalothrin and were overexpressed in a field-evolved lambda-cyhalothrin resistant population. Knockdown of CYP9A120 and CYP9A121 by RNA-mediated interference (RNAi) increased the susceptibility of larvae to lambda-cyhalothrin. In vitro assays demonstrated that recombinant P450s expressed in Sf9 cells can metabolize lambda-cyhalothrin, but with functional redundancy and divergence through regioselectivity of metabolism. CYP9A121 preferred to convert lambda-cyhalothrin to 2′-hydroxy-lambda-cyhalothrin, whereas CYP9A122 only generated 4′-hydroxy metabolite of lambda-cyhalothrin. Although possesses a relatively low metabolic capability, CYP9A120 balanced catalytic competence to generate both 2′- and 4′-metabolites. CONCLUSION: Collectively, these results reveal that metabolic functional redundancy of three members of the CYP9A subfamily leads to P450-mediated lambda-cyhalothrin resistance in C. pomonella, thus representing a potential adaptive evolutionary strategy during its worldwide expansion.

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Section: Introductionmentioning

confidence: 99%

Metabolic functional redundancy of the CYP9A subfamily members leads to P450-mediated lambda-cyhalothrin resistance in Cydia pomonella

Li¹,

Shi

Liu³

et al. 2022

Preprint

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“…Many of this family are phytophagous insects and highly harmful to crops or forests. Despite the large number of previous studies focusing on the morphology, physiology, and biological control of Noctuidae species [ 2 , 3 ], our understanding of the genomic diversity of the Noctuidae species, especially the transposable elements (TEs), is still in its infancy.…”

Section: Introductionmentioning

confidence: 99%

Transposable Elements Shape the Genome Diversity and the Evolution of Noctuidae Species

Zhang

Wang

et al. 2023

Genes

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Noctuidae is known to have high species diversity, although the genomic diversity of Noctuidae species has yet to be studied extensively. Investigation of transposable elements (TEs) in this family can improve our understanding of the genomic diversity of Noctuidae. In this study, we annotated and characterized genome-wide TEs in ten noctuid species belonging to seven genera. With multiple annotation pipelines, we constructed a consensus sequence library containing 1038–2826 TE consensus. The genome content of TEs showed high variation in the ten Noctuidae genomes, ranging from 11.3% to 45.0%. The relatedness analysis indicated that the TE content, especially the content of LINEs and DNA transposons, is positively correlated with the genome size (r = 0.86, p-value = 0.001). We identified SINE/B2 as a lineage-specific subfamily in Trichoplusia ni, a species-specific expansion of the LTR/Gypsy subfamily in Spodoptera exigua, and a recent expansion of SINE/5S subfamily in Busseola fusca. We further revealed that of the four TE classes, only LINEs showed phylogenetic signals with high confidence. We also examined how the expansion of TEs contributed to the evolution of noctuid genomes. Moreover, we identified 56 horizontal transfer TE (HTT) events among the ten noctuid species and at least three HTT events between the nine Noctuidae species and 11 non-noctuid arthropods. One of the HTT events of a Gypsy transposon might have caused the recent expansion of the Gypsy subfamily in the S. exigua genome. By determining the TE content, dynamics, and HTT events in the Noctuidae genomes, our study emphasized that TE activities and HTT events substantially impacted the Noctuidae genome evolution.

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“…While several studies have been devoted to assessing molecular mechanisms underlying pesticide resistance (see, e.g. the special issue in Insects, Le Goff & Nauen, 2021; Box 2), there is, however, an apparent knowledge gap on the significance of the ecological context of pesticide resistance evolution as well as on its ecological consequences (Chen & Schoville, 2018; Maino et al, 2018; Figure 2). A similar trend is observed when taking cross‐resistance into consideration, with less than 20 articles assessing either its ecological or evolutionary aspect.…”

Section: Introductionmentioning

confidence: 99%

Pesticide resistance in arthropods: Ecology matters too

et al. 2022

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Pesticide resistance development is an example of rapid contemporary evolution that poses immense challenges for agriculture. It typically evolves due to the strong directional selection that pesticide treatments exert on herbivorous arthropods. However, recent research suggests that some species are more prone to evolve pesticide resistance than others due to their evolutionary history and standing genetic variation. Generalist species might develop pesticide resistance especially rapidly due to pre‐adaptation to handle a wide array of plant allelochemicals. Moreover, research has shown that adaptation to novel host plants could lead to increased pesticide resistance. Exploring such cross‐resistance between host plant range evolution and pesticide resistance development from an ecological perspective is needed to understand its causes and consequences better. Much research has, however, been devoted to the molecular mechanisms underlying pesticide resistance while both the ecological contexts that could facilitate resistance evolution and the ecological consequences of cross‐resistance have been under‐studied. Here, we take an eco‐evolutionary approach and discuss circumstances that may facilitate cross‐resistance in arthropods and the consequences cross‐resistance may have for plant–arthropod interactions in both target and non‐target species and species interactions. Furthermore, we suggest future research avenues and practical implications of an increased ecological understanding of pesticide resistance evolution.

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Recent Advances in the Understanding of Molecular Mechanisms of Resistance in Noctuid Pests

Abstract: Noctuid moths are among the most devastating crop pests on the planet [...]

Cited by 8 publications

References 11 publications

Metabolic functional redundancy of the CYP9A subfamily members leads to P450-mediated lambda-cyhalothrin resistance in Cydia pomonella

Metabolic functional redundancy of the CYP9A subfamily members leads to P450-mediated lambda-cyhalothrin resistance in Cydia pomonella

Transposable Elements Shape the Genome Diversity and the Evolution of Noctuidae Species

Pesticide resistance in arthropods: Ecology matters too

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