Recent Advances in Virus Elimination and Tissue Culture for Quality Potato Seed Production

Naik, Prakash S.; Buckseth, Tanuja

doi:10.1007/978-3-319-78283-6_4

Cited by 31 publications

(19 citation statements)

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“…Sanitation, that is, removing viruses from valuable cultivars, is necessary when no healthy plants are available. Virus-free plants are usually produced by thermotherapy, chemotherapy, electrotherapy, and tissue culture alone or combined ( Naik and Buckseth, 2018 ). Thermotherapy could inactivate viruses by viral RNA breakage, viral particle disruption or coat protein rupture, viral replicase inactivation, virus movement inhibition and/or translation reduction ( Hull, 2002 ).…”

Section: Disease Managementmentioning

confidence: 99%

Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution

2020

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Plant viruses cause considerable economic losses and are a threat for sustainable agriculture. The frequent emergence of new viral diseases is mainly due to international trade, climate change, and the ability of viruses for rapid evolution. Disease control is based on two strategies: i) immunization (genetic resistance obtained by plant breeding, plant transformation, cross-protection, or others), and ii) prophylaxis to restrain virus dispersion (using quarantine, certification, removal of infected plants, control of natural vectors, or other procedures). Disease management relies strongly on a fast and accurate identification of the causal agent. For known viruses, diagnosis consists in assigning a virus infecting a plant sample to a group of viruses sharing common characteristics, which is usually referred to as species. However, the specificity of diagnosis can also reach higher taxonomic levels, as genus or family, or lower levels, as strain or variant. Diagnostic procedures must be optimized for accuracy by detecting the maximum number of members within the group (sensitivity as the true positive rate) and distinguishing them from outgroup viruses (specificity as the true negative rate). This requires information on the genetic relationships within-group and with members of other groups. The influence of the genetic diversity of virus populations in diagnosis and disease management is well documented, but information on how to integrate the genetic diversity in the detection methods is still scarce. Here we review the techniques used for plant virus diagnosis and disease control, including characteristics such as accuracy, detection level, multiplexing, quantification, portability, and designability. The effect of genetic diversity and evolution of plant viruses in the design and performance of some detection and disease control techniques are also discussed. High-throughput or next-generation sequencing provides broad-spectrum and accurate identification of viruses enabling multiplex detection, quantification, and the discovery of new viruses. Likely, this technique will be the future standard in diagnostics as its cost will be dropping and becoming more affordable.

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Section: Disease Managementmentioning

confidence: 99%

Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution

2020

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“…These initial plantlets, often called mother plants, are tested for all pathogens of concern, including the major potato viruses, potato spindle tuber viroid, and common bacterial and fungal pathogens (Frost et al 2013). Propagation in tissue culture is relatively inexpensive, requires little space, and the plantlets grow quickly, so hundreds of thousands of plantlets can be produced annually in a relatively small facility of tens to a few hundred square meters (Naik and Buckseth 2018). The micropropagated plantlets are then planted into greenhouses or screenhouses into either pots or into hydroponic or aeroponic systems.…”

Section: Potato Seed Systems In Europe and North Americamentioning

confidence: 99%

Potato Seed Systems

Forbes¹,

Charkowski

Andrade-Piedra

et al. 2020

The Potato Crop

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Good quality seed is almost universally considered a requirement for high productivity in all potato production systems. Much of the yield gap currently constraining productivity in low-income countries is attributed to the poor quality of seed. Potato seed sector development is thus a major concern of governments, researchers, development agencies, and civil society organizations. Potato seed systems are often characterized as formal or informal, although the informal seed system is complex and particularly in low income countries there are many linkages between the two systems. Informal seed potato systems in the Andes have existed for centuries, and for a number of reasons often produce seed of relatively high quality. In other low-income countries, informal systems produce seed of variable and frequently poor quality, contributing to very large yield gaps, characteristic of those areas. In regions of high potato productivity (e.g., the USA and Europe), formal systems, with seed of certified high quality, are dominant, although some productions subsectors (e.g., organic producers) often use seed that is not certified. Efforts to implement formal seed systems in low-income countries have been largely unsuccessful; consequently the vast majority of low-resource potato farmers source their seed via the informal system. Sectors of the development community

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“…The total sum of minitubers was calculated as the sum of all six harvests. For the production of seed potato minitubers, small sizes of 5 to 25 mm are usually accepted (Millam andSharma, 2007, cited by Naik andBuckseth, 2018;Struik, 2007). Minitubers of smaller sizes can be easily transported for commercialization as seed propagules to be further multiplied in greenhouses (nuclear seed).…”

Section: Data Collectionmentioning

confidence: 99%

Yield of Potato Minitubers under Aeroponics, Optimized for Nozzle Type and Spray Direction

Filho¹,

Fontes²,

Cecon³

et al. 2020

horts

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Potato seed production by conventional methods represents a sizeable investment that, when passed on to farmers, can decrease their profit margins. Potato minitubers produced by aeroponic systems are space- and cost-efficient, and they also provide healthy propagules to be used by farmers. We evaluated the effects of different misting nozzle types, with and without an antidrip feature, and spray direction on potato minituber yield using the Federal University of Viçosa (UFV) Aeroponic System. Potato plants (cv. Agata) propagated from sprouts were grown in a covered, high-density 100-L polyethylene bucket. The experiment was set up in a randomized complete block design with four replicates and eight treatments combining misting nozzle types (Fogger, MA-30, and CoolNet) with and without antidrip and comparing upward with downward spray directions. Plants were evaluated weekly from 33 to 68 days after transplant (DAT). The parameters used to evaluate treatments were number and mass of minitubers as a function of harvest times, dry mass of roots, stems, leaves, and total biomass. The number and fresh weight of minitubers, as well as root dry weight, stems, leaves, and total biomass were affected by misting nozzle types and spray direction. Treatments also affected biomass partitioning of roots, stem biomass, and the shoot:root ratio. There was also an effect of harvest time on the number and fresh weight of minitubers for various combinations of misting nozzle type and spray direction, except for minituber number with the CoolNet misting nozzle without antidrip and downward spray direction. On the basis of the assessed parameters, the best minituber production system was achieved with the Fogger spray combined with no antidrip, a rate of 12 L·h−1, and with the downward spray direction. The UFV Aeroponic System produced an average of 491 minitubers per plant. This system is simple to implement and may lead to a more affordable upscaling of potato seed minituber production.

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Recent Advances in Virus Elimination and Tissue Culture for Quality Potato Seed Production

Cited by 31 publications

References 50 publications

Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution

Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution

Potato Seed Systems

Yield of Potato Minitubers under Aeroponics, Optimized for Nozzle Type and Spray Direction

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